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L-精氨酸逆转一氧化氮抑制后的犬冠脉血流动力学改变和内皮素-1含量升高(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
1996年第4期
页码:
196-199
栏目:
论著
出版日期:
1996-08-01

文章信息/Info

Title:
Effects of intracoronary L-arginine on vasomotion and endothelium-dependent responses of coronary arteries and endothelin-1 levels after inhibition of nitric oxide in coronary circulation in anesthetized dogs
作者:
刘庆军1　王跃民2　臧益民2　吴明媚3
1解放军第91医院心内科 兖州 272000　第四军医大学 2生理学教研室　3唐都医院心内科
Author(s):
Liu Qingjun1; Wang Yaomin2;  Zang Yimin2; Wu Mingmei3
1Department of Cardiology, 91th Hospital of PLA , Yan Zhou 272000 2Department of Physiology, Fourth Military Medical University,Xi’an 710032 3Department of Cardiology, Tangdu Hospital, Fourth Military Medical University
关键词:
冠状动脉循环;  L-精氨酸;  一氧化氮;  内皮素;  血液动力学;  狗
Keywords:
coronary circulation;  L-arginine;  nitric oxide;  endothelin;  hemodynamics; dogs
分类号:
-
DOI:
-
文献标识码:
-
摘要:
观察了犬冠脉内灌注N-单甲基左旋精氨酸(L-NMMA )后再灌注L-精氨酸(L-Arg)和单独灌注L-NMMA前后冠脉血流动力学、冠脉血流储备以及冠脉对不同浓度的乙酰胆碱(Ach) 反应的变化, 同时用放免法测定冠脉前降支(LAD)伴行静脉血中内皮素-1(ET-1)含量。结果发现,L-Arg 完全逆转了灌注-NMMA 引起的血流动力学改变, 使心率回升, 下降的基础冠脉流量(CBF),从20±8 ml/min回升至28±7ml/min,P<0.05),降低的冠脉储备恢复, 从51±10 ml/min 升至94±15 ml/min, P0.01), ET-1 的含量不再升高, 从15.5±3.0 ng/L 下降至5.0±2.0 ng/L , P<0.01),Ach 介导的CBF增加不再受到抑制(P<0.01)。结果提示提供外源性L-Arg 可增加一氧化氮(NO) 的产生, 使由于NO 抑制而产生的血流动力学改变和ET-1升高发生逆转。
Abstract:
Peak reactive hyperemic coronary blood flow (CBF) following 15 s occlusion, resting CBF and CBF in response to acetylcholine (Ach) were observed both before and after a 5 mg/kg intracoronary infusion of N-monomethly-L-arginine (L-NMMA) and a 66 mg/kg of L-arginine (L-Arg) in open-chest anesthetized dogs,Meanwile, serial endothelin-1(ET-1) level was also obtained from the left interventricular vein and measured by radioimmuniassay. Results: L-Arg completely reversed heart rate, resting CBF and CBF, and peak reactive hyperemic CBF was decreased by L-NMMA. The increase in left interventricular vein blood ET-1 induced by L-NMMA were not observed any more for 60min after L-NMMA and L-Arg infusion. L-Arg also reversed the attenuated Ach-stimulated endothelium-dependent responses toward the levels before L-NMMA infusion. These results suggested that a supply of exogenous L-Arg could increase nitric oxide product, and reversed the change of hemodynamics and increased ET-1 levels after inhibition of nitric oxide.

参考文献/References

［1］刘庆军, 吴明媚, 臧益民, 等. N-单甲基左旋精氨酸对犬冠脉内皮依赖性舒张功能及内皮素-1含量的影响. 第四军医大学学报1994; 15 (4) : 261.

［2］Tomessen TT,Naess PA , Kirkeboen KA, et al.Release of endothelin from the porcine artery occlusion. Cardiovasc Res 1993; 27: 1482.

［3］曾 强, 李振甲, 余霞君, 等. 内皮素的放射免疫分析. 解放军医学杂志1991; 16 (6) : 403.

［4］Palmer RM T, Ashton D, Moncada S. Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature Lond 1988; 333: 664.

［5］Mitchell JA ,Hecker M ,Vane JR. The generation of L-arginine in endothelial cell is linked to t the release of endothelium-derived relaxing factor.Eur J Pharmacol 1990; 176: 253.

［6］Mark WI, James H, John C, et al. Circulating endothelin during acetycholine-induced coronary vasoconstriction in hypercholesterolemic pig. JACC 1993; 21 (2) : 125A.

［7］Luscher TF,Xang Z, Tschudi M,et al. Interaction between endothelin-1 and endothelium-derived relaxing factor in human arteries and veins. Cire Res 1990; 66: 1088.

［8］刘庆军, 吴明媚, 臧益民, 等. 犬冠脉血管顿抑时冠脉对乙酰胆硷的反应及内皮素-1含量的变化. 第四军医大学学报1993; 14 (6) : 404.

备注/Memo

备注/Memo:
Received 1996-02-05；revised 1996-09-21
更新日期/Last Update: 1996-08-01