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川芎嗪对再灌注大鼠心肌细胞c-fos基因表达及凋亡的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2000年第3期
页码:
213-215
栏目:
论著
出版日期:
2000-05-25

文章信息/Info

Title:
Effects of tetramethylpyrazine on c-fos expression and apoptosis of myocardial cells in myocardial ischemic reperfusion in rats
作者:
李源;  段红;  王天成;  张珊红;  龚卫琴
第四军医大学西京医院老年病科, 陕西西安710033
Author(s):
LI Yuan;  DUAN Hong;  WANG Tian-cheng;  ZHANG Shan-hong;  GONG Wei-qin
Department of Geriatrics, Xijing Hospital, Fourth Military Medical University, Xi′an 710033,Shaanxi,China
关键词:
心肌缺血再灌注; c-fos基因; 细胞调亡; 川芎嗪
Keywords:
myocardial ischemic reperfusion; c-fos expression; apoptosis; tetramethylpyrazine
分类号:
R331.31
DOI:
-
文献标识码:
A
摘要:
目的 观察川芎嗪（TMP）对心肌缺血再灌注（MIR）大鼠心肌c-fos基因表达及凋亡细胞的作用。方法 应用免疫组化法检测MIR不同时期心肌FOS蛋白的表达，并采用末端标记技术对心肌细胞凋亡数进行检测。结果 ①对照组心肌无FOS蛋白表达，亦未见凋亡细胞出现。②与MIR组相比，TMP干预组心肌FOS蛋白的表达明显减弱（60 min分别为57±44个/片和6.4±3.2个/片，90 min分别为467±450个/片和171±160个/片，120 min分别为246±160个/片和130±121个/片，均P＜0.05），TUNEL阳性细胞数均明显减少（60 min分别为36.3±8.7个/片和24.7±7.2个/片,P＜0.05; 120 min分别为48.0±23.8个/片和10.0±8.1个/片, P＜0.01）。结论 MIR可诱导终末分化的心肌细胞高表达FOS蛋白及发生细胞凋亡；应用TMP可使缺血心肌细胞c-fos基因表达减弱及凋亡减轻。
Abstract:
AIM To study the effects of tetramethylpyrazine (TMP) on c-fos expression and apoptosis of myocardial cells in myocardial ischemic reperfusion (MIR) in rats. METHODS Coloring TDT-mediated dutp nick end labeling (TUNEL) and immunohistochemical staining were used. RESULTS ①No blue apoptosis cells and FOS protein expression were found in non-ischemic myocardial tissues; ②Compared with those of MIR rats, the number of FOS immunoreactive positive cells in rats treated with TMP decreased significantly (from 57±44/section to 6.4±3.2/section, 467±450/section to 171±161/section and 246±160/section to 130±121/section respectively, P＜0.05 ) in 60, 90 and 120 min and the number of TUNEL positive cells in rats treated with TMP also decreased significantly ( from 36.3±8.7/section to 24.7±7.2/section and 48.0±23.8/section to 10.0±8.1/section respectively, P＜0.01) in 60 minutes and 120 minutes. CONCLUSION Myocardial cells may induce apoptosis in MIR and apoptosis may be related to the increased c-fos expression. Administration of TMP can decrease the expression of c-fos and the apoptosis of myocardial cells in MIR.

参考文献/References

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［6］ 王玉良, 巴彦坤. 川芎嗪对心血管的药理和电生理作用—一种新的钙拮抗剂［J］. 中西医结合杂志, 1995,15(6):291.

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备注/Memo

备注/Memo:
收稿日期：1999-04-15.
更新日期/Last Update: 2000-05-25