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|本期目录/Table of Contents|

大鼠钠尿肽B受体cDNA探针克隆载体的构建(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2000年第5期
页码:
387-388, 391
栏目:
博士之窗
出版日期:
2000-10-25

文章信息/Info

Title:
Construction of cDNA probe clone vector for natriuretic peptide B receptor
作者:
董明清1;  朱妙章1;  于军1;  高瞻1;  吕顺燕1;  郭海涛1;  冯华松2
第四军医大学: 1. 基础部生理学教研室, 陕西西安710032, 2. 唐都医院呼吸内科
Author(s):
DONG Ming-qing1;  ZHU Miao-zhang1;  YU Jun1;  GAO Zhan1;  LU Shun-yan1;  GUO Hai-tao1;  FENG Hua-song2
1Department of Physiology, 2Department of Respiratory, Tangdu Hospital, Fourth Military Medical University, Xian 710032, Shaanxi, China
关键词:
受体; 钠尿肽; 克隆; cDNA探针
Keywords:
receptor;  natriuretic peptide;  clone;  cDNA probe
分类号:
R33
DOI:
-
文献标识码:
A
摘要:
目的 构建大鼠钠尿肽B受体cDNA探针克隆载体，为研究该受体 基因表达奠定基础。方法 设计钠尿肽B受体特异的PCR引物，提取大鼠肺组织 总RNA，进行反转录PCR，扩增目的片段，回收目的片段，将其克隆入pGEM-T载体，酶切鉴 定并用双脱氧法测序。结果 PCR反应产物经琼脂糖凝胶电泳鉴定与所设计引物 大 小一致，克隆入pGEM-T载体后酶切可切出所需片段，测序证实为所设计序列。结 论 构建的pGEM-T重组质粒为进一步研究该受体基因表达、分布等创造了条件。
Abstract:
AIM To construction of cDNA probe clone vector for B receptor of natriuretic peptide. METHODS SD rat lungs were employed to clone the fragment of natriuretic peptide B receptor. Total RNA molecules were i solated from fresh lungs and mRNA were reversely transcriped into cDNAs. After a mplification by PCR,the fragment of DNA were cloned into vector pGEM-T. The met hod of dideoxy-termination was employed to sequence. RESULT The gene fragment of B receptor of natriuretic peptide was successfully cloned in pGEM-T vector and the sequence was correct. CONCLUSION The clone vector constr ucted can be a source of cDNA probe for natriuretic peptide B receptor and was u seful in study its gene express.

参考文献/References

［1］ Li H, Oparil S, Meng QC, et al. Selective downregulation of AN P -clearance receptor gene expression in lung of rats adapted to hypoxia［J］. Am J physiol,1995,268(2 pt 1):1328.

［2］ 萨姆布鲁克 J，弗里奇 EF，曼尼阿蒂 T, et al. 分子克隆实验指南［M］. 第2 版. 北京：科学技术出版社，1996.

［3］ Lisy O, Burnett JC Jr. Potential future therapies: natriuretic peptide s［J］. Coron Artery Dis, 1999，10(6):389.

［4］ Schrier RW, Abraham WT. Hormones and hemodynamics in heart failure［J ］. N Engl J Med, 1999,341(8):577.

［5］ Mills RM, Lejemtel TH, Horton DP, et al. Sustained hemodynamic eff ects of an infusion of nesiritide (human b-type natriuretic peptide) in heart f ailure: a randomized, double-blind, placebo-controlled clinical trial. Natreco r Study Group［J］. J Am Coll Cardiol, 1999, 34(1):155.

［6］ Sasaki T, Kubo T, Komamura K, et al. Effects of long-term treatment with pimobendan on neurohumoral factors in patients with non-ischemic chronic m oderate heart failure［J］. J Cardiol, 1999, 33(6):317.

［7］ Mair J, Friedl W, Thomas S， et al. Natriuretic peptides in assessment of left-ventricular dysfunction［J］. Scand J Clin Lab Invest Suppl， 1999 ，230:132

备注/Memo

备注/Memo:
收稿日期：1999-12-29.基金项目：国家自然科学基金资助项目No. 39770317, No. 39970327
更新日期/Last Update: 2000-10-25