褪黑素对心肌细胞抗过氧化氢损伤的实验研究(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2001年第1期
- 页码:
- 37-40
- 栏目:
- 论著
- 出版日期:
- 2001-01-25
文章信息/Info
- Title:
- Effects of melatonin on cultured rat cardiomyocytes anti-injury mediated by H202
- Author(s):
- ZHENG Yan-song1 ; LI Yuan1; ZANG Yi-min2 ; GONG Wei-qin 1; SUN Bin1
- 1．Department of Geriatrics，Xijing Hospital，2，Department of Physiology．Fourth Military Mediea1 University，Xi'an Shaanxi 710032，China
- 分类号:
- R330.36
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 观察H2O2对心肌细胞内活性氧(ROS)和游离钙([Ca2+] )的影响盈褪黑索(MT)的保护作用．方法 酶清化法原代分离培养乳鼠心肌细胞，分别用荧光探针DCFH-DA和F]uo-3-AM 标记，H2O2和MT处理后，流式细胞仪检测细胞荧光强度的变化。结果 低维度的H2O2(1OOumol／L)随着时间的延长可使心肌细胞内的ROS呈上升趋势，3Omin，60min与0 min或对照组相比均明显增高(P<0．01)；MT干预后上升趋势均明显减弱．在30，60min两组均差异显著(P
- Abstract:
- AIM T0 investigate the role of H2O2 in myocardial intracellular reactive oxygen species (ROS)and free calcium([Ca2+])and the effects of melatonin(MT)on them．METHODS The fluorescent probe DCFH-DA was used to detect intracellular levels of ROS and Fluo-3-AM to detect[Ca 2+]．Cultured rat ventrlcular myocytes were divided into 4 groups：Control group，H202 group，MT group and MT+ H2O2 group．Cells were incubated with DCFH-DA or Fluo-3-AM prior to the exposure to l00umol／L H2O2 and／or MT．The changes in eel1 fluorescence were measured by flow cytomerer(FCM )at different time points，RESULTS Compared with the control group，H2O2 at low c0ncentrati0n(100 vmol／I )caused significant time-dependent increase in intracellular ROS and[Ca2+(ROS:30 min，742士326 vs 200士130，P<0．01，60 min，l630士421 vs 198士l30，P<0.0l;[Ca2+]：3O min，126士l14 vs 121士99，P<0．05，60 min，844士356 vs 121士106，P< 0．01)．However，those changes were significantly attenuated in MT+ H2O2 group(ROS；30 min，742士326 vs 223士125，P< 0．O1，60 min，1630士421 vs 317士2ll，P< 0．01; [Ca2+]：60 min，844士356 vs 439士261．P<0．O1)．CONCLUSI0N lntracellular R0S and [Ca2+] increase when cardiomyocytes are challenged by 100umol／L H2O2．MT has very good antloxldant effect and can be used to protect cardiomyoeytes．
参考文献/References
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备注/Memo
- 备注/Memo:
- 收稿日期：2000-05-22.
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