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TGF-β对动脉钙化的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2001年第3期
页码:
170-172
栏目:
论著
出版日期:
2001-05-01

文章信息/Info

Title:
Transforming growth factor-β increased the calcium deposition of vascular calcification in vitro
作者:
王宇玫;  王士雯;  佟万仁;  丁秀云;  陈　宇
解放军总医院老年心血管病研究所, 北京100853
Author(s):
WANG Yu-mei;  WANG Shi-wen;  TONG Wan-ren;  DING Xiu-yun;  CHEN Yu
Institute of Geriatric Cardiology, 301 Hospital, PLA, Beijing 100853, China
关键词:
钙化;  动脉;  转化生长因子2B;  骨钙素;  动脉粥样硬化
Keywords:
vascular calcification;  transforming growth factor β;  osteocalcin;  vascular smooth muscle cell;  bovine
分类号:
R542.5
DOI:
-
文献标识码:
A
摘要:
目的 探讨转化生长因子 - β(TGF- β)在动脉钙化中的作用。方法 建立体外动脉钙化的模型 ,培养钙化血管平滑肌细胞 ,在培养液中加入 TGF- β,观察钙沉积及细胞碱性磷酸酶的变化 ,同时观察钙化过程中骨间质蛋白骨钙素分泌情况。结果 1μg/ L 的 TGF- β作用 10 d后 ,钙化细胞的钙沉积为 1.95± 0 .5 6 μm ol/ L,较钙化对照组 (1.71±0 .0 5 μm ol/ L)增加了 14.4% ,细胞碱性磷酸酶的活性为 5 46± 118IU/ L,比钙化对照组 (16 5± 6 3IU/ L)增加了2 95 % ,这种作用在第 8天达到高峰 ,以后略有下降。在钙化加重的同时 ,骨钙素分泌亦明显增加 ,达 0 .37± 0 .0 4ng/ L;而 TGF- β对非钙化的细胞无上述作用。结论 1μg/ L 的 TGF- β可以促进钙化血管平滑肌细胞的钙化过程。
Abstract:
AIM To investigate the role transforming growth factor β (TGF-β) in the vascular calcification. METHODS Using β glycerin phosphate sodium salt to induce cultured bovine vessel smooth muscle cell to calcify and this serves as a vascular calcifying model in vitro. TGF-β were add to the culture media. The deposition of calcium,alkaline phosphatase activity and osteocalcin concentration was quantified during the 10d culture processes. RESULTS After 10 d of culture,1 μg/L TGF β increased the calcium deposition of calcified VSMC from 1.71±0. 05 Lmol/L to 1.95 ±0. 56 Lmol/L. The cellular ALP activity was significantly increased from 165±63 IU/L of calcified control group to 546±118 IU/L with the maximum increase in the 8 d. The osteocalcin concentration of cultured media syntheses by BVSMCs was also elevated to 0.37±0. 04 ng/L after TGF2B treatment. CONCLUSION TGF-β increased calcified BVSMCs calcium deposition via stimulating the cellular synthesis of ALP and osteocalcin and promoting the change of calcified BVSMC to osteoblastic cell.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2000-07-24.
更新日期/Last Update: 2001-05-01