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|本期目录/Table of Contents|

心肌缺血/再灌注损伤时抗凝血酶和纤维蛋白原的变化(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第4期
页码:
301-302,304
栏目:
实验研究
出版日期:
2002-07-01

文章信息/Info

Title:
Changes in antithrombin and fibrinogen during myocardial ischemia/reperfus ion injury
作者:
戴　菁;  王明山
温州医学院附属第一医院检验科, 浙江 温州 325000
Author(s):
DAI Jing ; WANG Ming-shan
Affiliated First Hospital,Wenzhou Medical College,Wenzhou, Zhejiang 325000, China
关键词:
缺血/再灌注损伤;  抗凝血酶? ;  纤维蛋白原;  纤溶酶原
Keywords:
ischem ia/reperfusion injury;  antithrom bin2Ⅱ ;  fibrinogen;  plasminogen
分类号:
R543.31
DOI:
-
文献标识码:
A
摘要:
目的 探讨心肌缺血/再灌注损伤时抗凝血系统的变化及纳洛酮的影响。方法 利用兔心肌缺血?再灌注模型观察心肌缺血?再灌注损伤时, 抗凝血酶Ⅱ (AT2Ⅱ )、纤维蛋白原(FIB) 和纤溶酶原(PIG) 动态变化及用阿片受体拮抗剂—纳洛酮保护时它们的变化情况。新西兰兔27 只, 随机分成3 组(实验组、保护组和对照组) , 每组9 只, 对照组分别于拮扎前、后0. 5, 1, 2, 4, 6 h 采血, 实验组和纳洛酮保护组分别于结扎前、后30 min 松开结扎线恢复灌流时及灌注后0. 5, 1, 2, 4, 6 h 采血。采用发色底物法测定AT2Ⅱ , PLG 活性、凝血酶原时间(PT) , 衍生法测定PIB 含量。结果 心肌缺血后各时间段AT2Ⅱ , FIB, PLG 的变化不明显, 但心肌缺血/再灌注后0. 5, 1, 2, 4, 6 h 实验组与实验保护组AT2Ⅱ , PLG 活性、F B 含量均逐渐下降, 实验组与纳洛酮保护组各指标的比较无显著性差异。结论 心肌缺血/再灌注损伤早期能使F B 含量及AT2Ⅱ , PLG 活性出现消耗性降低, 纳洛酮对抗凝血系统的影响不明显。
Abstract:
AIM To study the changes in anticoagulation system and the effect of neloxone during myocardial ischemia/reperfusion injury.METHODS The rabbit models with myocardial ischemia/reperfusion were used to observe the changes in AT-Ⅲ, FIB and PLG during the reperfusion period and when they were protected by neloxone. Twenty-seven New Zealand rabbits were randomly divided into GroupsⅠ,Ⅱ,Ⅲ. Regional myocardial ischemia(RMI) was produced by the ligation of left anterior descending of coroary artery. The subjects in GroupsⅠ serving as controls were detected before and 0.5,1,2,4 and 6 h after the ligation. The subjects in the other two groups were detected before ligation, 30 min after the ligation, during the reperfusion and 0.5,1,2,4 and 6 h after the reperfusion. The activities of AT-Ⅲand PLG were measurd by luminescence analysis. The level of FIB was analyzed by PT derived fibrinogen. RESULTS Changes in AT-Ⅲ,FIB and PLG after myocardial ischemia at each time point were not very apparent. But the activities of AT-Ⅲand PLG and the levels of FIB all decreased slowly in GroupsⅡandⅢat 0.5,1,2,4 h and 6 h after the myocardial ischemia/reperfusion. The items in GroupsⅡandⅢshowed no marked diversity.CONCLUSION The levels of FIB and the activities of AT-Ⅲand PLG consumptively decreaed during early myocardial ischemia/reperfustion injury. The effect of neloxon on anticoagulation system was not apparent.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期: 2001-07-26.
更新日期/Last Update: 2002-07-01