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|本期目录/Table of Contents|

慢性高同型半胱氨酸血症兔氧化应激在血管内皮功能损伤中的作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2003年第4期
页码:
311-312,315
栏目:
实验研究
出版日期:
2003-07-01

文章信息/Info

Title:
Oxidant stress on the endothelial dysfunction of the chronic hyperhomocysteinemia rabbits
作者:
阮骊韬; 曹铁生; 段云友; 庄磊
第四军医大学唐都医院超声科,陕西 西安 710038
Author(s):
RUAN Li-tao;  CAO Tie-sheng;  DUAN Yun-you;  ZHUANG Lei
Department of Ultrasound diagnotics, Tangdu Hospital, FMMU, Xi'an, Shaanxi 710038, China
关键词:
高同型半胱氨酸血症; 一氧化氮; 谷胱甘肽过氧化物酶; 内皮依赖性舒张功能; 内皮非依赖性舒张功能; 超声
Keywords:
hyperhomocysteinemia;  nitric oxides;  glutathione peroxidase;  endothelium dependent dilation;  endothelium-dependent dilation;  ultrasound
分类号:
R541.1
DOI:
-
文献标识码:
A
摘要:
目的:探讨慢性高同型半胱氨酸血症(HHcy)导致内皮功能损伤时,兔血中一氧化氮(NO)以及谷胱甘肽过氧化物酶(GSH-PX)活性的改变,从而观察氧化应激在内皮功能损伤中的作用。方法:将兔随机分为两组,每组9只,分别喂食正常饲料(N组)和L-蛋氨酸(0.8g·kg-1·d-1)饲料(M 组),于第20,40,60,80d取血检测NO和GSHPX的活性,同时利用高分辨力超声检测其内皮依赖性舒张功能(EDD)、内皮非依赖性舒张功能(EID)的改变。结果:慢性HHcy时,M 组兔EDD、EID均较N组明显降低(分别为P<0.01,P<0.05),相应的血浆NO、GSH-PX活性亦较N组明显降低(P<0.05)。结论:同型半胱氨酸(Hcy)通过氧化应激灭活NO并破坏抗氧化酶GSH-PX导致内皮功能的损伤。
Abstract:
AIM: To observe the changes in nitric oxides (NO) and activity of glutathione peroxidase (GSH-PX) in plasma of hyperhomocysteinemia (HHcy) rabbits so as to study the effects of oxidant stress on endothelialdysfunction. METHODS: Eighteen rabbits were randomized into two groups (n=9pergroup). The normal control group (N group) received normal diet, and the methioine group (M group) received methioine-rich diet containing L methioine 0.8g·kg-1·d-1. Then NO and activity of GSH-PX in plasma were examined on the 20th, 40th, 60th and 80th days. At the sametime, endothelium-dependent dilation (EDD) and endothelium-independent dilation (EID) were examined by high resolution ultrasound. RESULTS: In chronic hyperhomocysteinemia, EDD and EID of external iliac arteries in the M group were reduced significantly compared with those of the N group. At the same time, NO and GSH-PX in the M group were also reduced significantly compared with those fo the N group. CONCLUSION: Through oxidant stress, Hcyin actived NO and destroyed GSH-PX, leading to endothelial dysfunction.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2002-05-20.
更新日期/Last Update: 2003-07-01