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伊贝沙坦对血管平滑肌细胞分泌细胞因子的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2003年第5期
页码:
394-396
栏目:
实验研究
出版日期:
2003-09-01

文章信息/Info

Title:
Effects of irbesartan on cytokins secretion by rabbit vascular smooth muscle cells
作者:
吴兴利;  王士雯;  施伟伟;  杨丁友;  高玉玲;  赵玉生
解放军总医院老年心血管病研究所, 北京 100853
Author(s):
WU Xing-li;  WANG Shi-wen;  SHI Wei-wei;  YANG Ding-you;  GAO Yu-ling;  ZHAO Yu-sheng
Institute of Geriatric cardiology, General Hospital of PLA,Beijing 100853, China
关键词:
脂蛋白类; LDL; 伊贝沙坦; 血管平滑肌细胞; 细胞活素类
Keywords:
irbesartan;  vascular smooth muscle cell;  cytokines
分类号:
R363　
DOI:
-
文献标识码:
A
摘要:
目的:探讨伊贝沙坦(Irb) 对血管平滑肌细胞(VSMC)分泌细胞因子的影响。方法:兔VSMC分6组处理,即对照组,低密度脂蛋白组,氧化低密度脂蛋白(oxLD) 组, Irb不同浓度组, Irb 不同时间组及Irb+ oxLDL组。以酶联免疫法检测培养上清液中细胞因子TN FA, IL 26, IL 28 水平。结果:oxLDL (50 mg?L) 对TN FA,IL 26,IL28分泌水平于刺激后8h增加至6.4～8.5倍; Irb 呈浓度(0. 01～ 100 Lmo l?L )、时间(1～ 24 h) 依赖地抑制基础及oxLDL 刺激的细胞因子分泌(与对照组比较均P<0. 05)。结论: Irb可能通过抑制基础及oxLDL刺激的细胞因子分泌对VSMC生物学功能发挥调节作用。
Abstract:
AIM: To investigate the effects of irbesartan (Irb) on cytokins secretion by vascular smooth muscle cells (VSMC). METHODS: Rabbit aortic VSMCs were cultured in 6 groups: control, the low density lipoprotein, the oxidized low density lipoprotein (oxLDL) , the Irb +oxLDL and the Irb of different concent rations and time groups. The level of tumor necrosis factor alpha (TNFA), interleukin-6 (IL-6) and in terleukin-8 (IL-8) were evaluated by ELISA method. RESULTS: oxLDL increased the secretion of TNFA, IL-6 and IL-8 by 6.4～ 8.52 fold in 8h. Irb decreased the cytockins secretion in a concent ration (0.01～100Lmol?L)-and time (1～24h)-dependent manner, and inhibited the above effects of oxLDL. CONCLUSION: Irb could regulate the biological effects of VSMC by inhibiting base and oxLDL-stimulated cytokins secretion.

参考文献/References

[1] Ross R. Atherosclerosis—an inflammation disease [J] . N Engl J Med , 1999, 340(2):115- 126.

[2] Rosendorff C. The renin-angiotensin system and vascular hypertrophy [J] . J Am Coll Cardiol, 1996, 28:803-812.

[3] Kodama H, Fukuda K, Pan J , et al . Biphasic activation of the JA K?STA T pathway by angiotensin ? in rat cardiomyocytes [J] . Circ Res, 1998, 82:244-250.

[4] Oparil S, Guthrie R, Lowin AJ , et al. An selective-titration study of the comparative effectiveness of two angio tensin? receptor blockers, Irbesartan and Losarten [J] . Clin Ther, 1998, 20(3):398-409.

[5] 吴兴利, 王士雯, 杨中苏, 等. 氧化低密度脂蛋白对血管平滑肌细胞生物学功能改变的影响及阿托伐他汀干预作用 [J]. 军医进修学院学报, 2002, 23(4):320- 321.

[6] 赵三妹, 夏人仪, 王宗立, 等. 动脉平滑肌细胞的培养方法及其应用 [J]. 中华病理学杂志, 1987, 16(4):260-261.

[7] Chung BH, Wilkinson T, Geer JC, et al. Preparative and quantitative isolation of plasma lipoproteins: rapid single discontinuous density gradient ultracentrifugation in a vertical rotor [J] . J Lipid Res, 1980, 21:284-291.

[8] Ito T, Ikeda U , Sh impo M. Serotonin increases interleukin-6 synthesis in human vascular smooth muscle cells [J] . Circulation, 2000, 102(20):2522- 2527.

[9] Simonini A, Mo scucci M, M uller DW , et al. IL-8 is an angiogenic factor in human coronary atherectomy tissue [J] . Circulation, 2000, 101(3):1519- 1526.

[10] Iouzalen L, Stepien O, Marche P. Effects of BAY 10～6734 (Embusartan), a new angiotensin ? type ? receptor antagonist, on vascular smooth muscle cell growth [J] . J Pharmacol Exp Ther, 1999, 289(1):181.

[11] Oparil S. New ly emerging pharmacologic differences in angiotensin ? receptor blockers [J] . Am J Hypertens, 2000, 13(1Pt2) : S18- S24.

备注/Memo

备注/Memo:
收稿日期：2002-07-22.
更新日期/Last Update: 2003-09-01