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胰岛素对自发性高血压大鼠离体主动脉的舒张作用及机制(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2003年第6期
页码:
501-503,507
栏目:
实验研究
出版日期:
2003-11-01

文章信息/Info

Title:
Vasodilatory effect and mechanism of insulin on isolated aorta in normal and spontaneously hypertensive rats
作者:
李榕1; 姬秋和1; 马恒2; 朱妙章2; 高峰2
第四军医大学: 1.西京医院内分泌科, 2.基础部生理学教研室, 陕西　西安 710032
Author(s):
LI Rong1;  JI Qiu-he1;  MA Heng2;  ZHU Miao-zhang2;  GAO Feng2
1.Department of Endocrinology, Xijing Hospital, 2.Department of Physiology, Fourth Military Medical University, Xi’an, Shaanxi 710032, China
关键词:
胰岛素; 高血压; 一氧化氮; 主动脉; 血管内皮细胞
Keywords:
insulin;  hypertension;  nitric oxide;  vascular endothelium cell
分类号:
Q463　　
DOI:
-
文献标识码:
A
摘要:
目的:探讨自发性高血压大鼠(SHR)的动脉血管对胰岛素(Ins)反应性的改变及其主要机制。方法: 采用离体血管灌流方法, 对比观察成年(15 周龄) 和幼年(5周龄)自发性高血压大鼠以及成年和幼年正常大鼠(WKY) 的胸主动脉环, 在未加干预或施加干预(机械法去除血管内皮或用一氧化氮合酶抑制剂处理) 后对Ins 反应的变化。结果: Ins(1～120U ?L) 对成年和幼年WKY胸主动脉血管具有舒张作用, 而对相应年龄SHR 的舒张作用均降低。在120 U ?L Ins 时, 成年SHR 和WKY大鼠的血管收缩率为: 73%±4% vs 29% ±4% , P<0.01; 幼年SHR 和W KY大鼠的血管收缩率为: 61%±5% vs 32% ±6% , P<0.01。去除内皮或用10 Lmo l?L 一氧化氮合酶抑制剂处理后,Ins 对成年和幼年W KY 胸主动脉血管的舒张作用均降低, 且和相应周龄的SHR 无显著差异。此外, 成年SHR 动脉环在去除内皮前后对Ins 的反应无显著差异(73% ±4% vs 82% ±6% , P>0.05) , 而幼年SHR 在去除内皮后对Ins 的反应显著降低(61% ±5% vs 78% ±5% , P<0.05)。结论: Ins 对血管的舒张作用是部分通过内皮和一氧化氮(NO) 介导的。成年SHR 血管内皮细胞功能受损, 而幼年SHR内皮功能无明显损害。此外, 两者可能都存在胰岛素抵抗的状态, 从而降低了血管对Ins 的敏感性。
Abstract:
AIM: To observe the effect of insulin on vascular tone in hypertension conditions. METHODS: SHR before (5 weeks old) and after (15 weeks old) the establishment of hypertension and two groups of age-and sex-matched WKY were studied. The vasodilation induced by increasing doses of insulin (1 to 120 U?L ) was tested on aortic rings. RESULTS: The vasodilation evoked by insulin was impaired in both the 5-week-old and 15-week-old SHR compared with the age-and sex-matched WKY [% of contraction (in 120 U?L Ins): 5-week-old, SHR61±5 vs WKY 32±6, P<0.01; 15-week-old, SHR 73±4 vs WKY 29±4, P<0.01 ]. After the removal of endothelium or the inhibition of endothelial NO synthase, the vasodilation evoked by the hormone was blunted and became similar between hypertensive and normotensive rats in both the 5-week-old and 15-week-old groups. CONCLUSION: ① Insulin has inhibitory effect on vascular tone. The effect is, partly at least, endothelium or NO-dependent. ② The vasorelaxan tresponses to insulin were significantly blunted in both the young and adult SHR, suggesting that endothelium may be impaired in hypertension and the resistant states towards in sulin may exist even before the occurrence of hypertension in SHR.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2002-12-30.
更新日期/Last Update: 2003-11-01