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心房和心室M受体密度的种属差异性比较(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第3期
页码:
210-212
栏目:
基础研究
出版日期:
2005-05-05

文章信息/Info

Title:
The comparison of Macetycholine receptors in atrial and ventricular myocardium of rats, guineapig and rabbits
作者:
曾菊绒; 臧伟进; 林元喜; 于晓江; 陈莉娜; 康新勤; 吕军
西安交通大学医学院药理学教研室，陕西 西安 710061
Author(s):
ZENG Jurong;  ZANG Weijin; LIN Yuanxi; YU XiaoJiang; CHEN Lina; KANG Xinqin; L Jun
Department of Pharmacology, Xi’ an Jiao Tong Medical University, Xi’ an, Shaanxi 710061, China
关键词:
M受体; 心房肌; 心室肌; 放射性配基受体结合法; 种属
Keywords:
Macetylcholine receptors; atrial and ventricular myocardium; radioligand binding assay; species
分类号:
R544;R96;R332
DOI:
-
文献标识码:
A
摘要:
目的 比较大鼠、豚鼠和家兔心房肌和心室肌M受体分布密度及与其强阻断剂氚标记二苯羟乙酸奎宁酯（3HQNB）亲和活性的种属差异性。方法 运用放射性配基受体结合法测定不同动物心房肌和心室肌M受体密度及解离常数（Kd）值。结果 ①大鼠心房M受体的分布密度约为心室的3.0倍，家兔心房是心室的4.0倍，而豚鼠心房是心室的1.7倍。②M受体在不同种动物心房肌密度排序：家兔＞大鼠＞豚鼠。心室肌密度排序：豚鼠＞大鼠＞家兔。Kd值在心房、心室及种间均无统计学差异。结论 ①M受体在大鼠、豚鼠和家兔心脏的分布密度不仅具有房室间差异性，而且还有种间差异性。②M受体与其阻断剂3HQNB亲和活性的种间差异性不明显。
Abstract:
AIM To investigate the density of Macetylcholine receptors in atrial and ventricular myocardium of rats, guineapig and rabbits. METHODS Using radioligand binding assay, the Mreceptor density and dissociation constant were observed in atrial and ventricular myocardium of above animals. RESULTS ① Atrial myocardial Mreceptors density is 3.0, 4.0 and 1.7 times bigger than ventricular in rats, rabbits and guineapig respectively. ② The density of Mreceptors in atrial myocardium from high to low is: rabbits, rats and guineapig. whereas ventricular myocardium was found to be: guineapig, rats and rabbits. ③ No significant difference were observed in Kd of atrial and ventricular myocardium of rats, rabbits and guineapig. CONCLUSION The density of Mreceptors is not only different in atrial and ventricular myocardium in animals mentioned above, but also difference among species. In addition, the affinity of M receptors was found to be no speciesrelated.

参考文献/References

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［2］曾菊绒，臧伟进，臧益民，等. 心脏毒蕈碱样乙酰胆碱受体研究进展［J］，心脏杂志，2004，（16）4：371-374

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［9］方萍，臧伟进，于晓江，等. 乙酰胆碱对豚鼠心房肌和心室肌的动作电位和收缩力的不同作用［J］. 生理学报,2002, 54(4): 311-316.

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备注/Memo

备注/Memo:
收稿日期：2004-12-23.基金项目：国家自然科学基金（编号30270554,30470633）；教育部科学技术重点项目(编号01161)；教育部高校博士学科点基金资助项目（编号20010698034）
更新日期/Last Update: 2010-01-05