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成熟心肌细胞异体移植对心肌梗死后心功能的保护作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第3期
页码:
225-228
栏目:
基础研究
出版日期:
2005-05-05

文章信息/Info

Title:
Adult cardiomyocytes allograft to protect the cardiac function after myocardial infarction
作者:
王晨;  王海昌;  张荣庆
第四军医大学西京医院心脏内科，陕西 西安 710032
Author(s):
WANG Chen;  WANG Haichang;  ZHANG Rongqing
Department of Cardiology, Xijing Hospital， Fourth Military Medical University,XI′an， Shaanxi 710032,China
关键词:
心肌细胞移植; 心肌梗塞; 左室重构
Keywords:
cardiomyocytes transplantation;  myocardial infarction;  left ventricular remolding
分类号:
R542.22
DOI:
-
文献标识码:
A
摘要:
目的 研究异体成熟心肌细胞移植对心肌梗死后心功能的保护作用。方法 成年新西兰兔结扎冠状动脉左前降支建立心肌梗死模型，体外培养异体心肌细胞并用5溴脱氧尿核苷（BrdU）标记，将4×107 /ml细胞悬液移植到急性心肌梗死区域。对照组将同量细胞培养液注射到急性心肌梗死区。细胞移植5周后超声心动图测量活体心功能，并进行组织学观察及免疫组化检测。结果 接受细胞移植组心脏功能显著优于对照组，左室重构受到抑制。细胞移植区观察到被标记心肌细胞，同时电镜下观察到心肌细胞之间存在细胞间联系。结论 移植细胞能够在宿主体内存活并与周围组织建立联系抑制左室重构，保护心脏功能。
Abstract:
AIM In order to research the effect of transplantation of homologous adult cardiomyocytes on protecting heart function after myocardial infarction(MI).METHODS After labeled with BrdU,cultured adult rabbit cardiomyocytes were implanted into the infarction site of rabbits myocardiums via direct injection. The myocardial infarction model was established by ligating the left anterior descending artery. All groups heart function and ventricular remodeling were measured by echocardiography, and the heart slices were processed for histological(HE)and immunohistological techniques and photographed. RESULTS In celltransplanted group heart functions were improved and left ventricular(LV) remoldings were inhibited.Transplanted cells labeled with BrdU were observed by microscope. In addition that implanted cells connected with each other by intercellular junctions. CONCLUSION Transplanted cells can live in the MI sections and improve heart functions.

参考文献/References

［1］ AlRadi OO, Rao V, Li RK, et al. Cardiac cell transplantation: closer to bedside［J］. Ann Thorac Surg,2003 ,75(2):S674-S677.

［2］ 杨胜利,何作云. 干细胞移植治疗心肌梗死［J］. 心脏杂志,2003,15(4):372-373.

［3］ 张航向,李源,臧益民. 干细胞移植与心血管疾病［J］. 心脏杂志，2002,14(6):524-529.

［4］ Stamm C, Westphal B, Kleine KD, et al. Bone marrow stem cell transplantation for myocardial regeneration in postinfarction CABG patients［J］. Circulation,2002,106 (suppl II)（Abstract）： II-375.

［5］ Min JY, Yang YK, Converso KL,et al. Transplantation of embryonic stem cells improves cardiac function in postinfarcted rats［J］. J Appl Physiol, 2002,92: 288-296.

［6］ Perin EC, Dohmann HFR, Borojevic R, et al. Transendocardial, autologous bone marrow cell transplantation for severe, chronic ischemic heart failure［J］. Circulation,2003,107: 2294-2302.

［7］ Zhang M, Methot D, Poppa V. Cardiomyocyte grafting for cardiac repair: graft cell death and anti-death strategies［J］. J Mol Cell Cardiol,2001,33: 907-921.

［8］ El Oakley RM, Ooi OC, Bongso A, et al. Myocyte transplantation for myocardial repair: a few good cells can mend a broken heart［J］. Ann Thorac Surg,2001, 71: 1724-1733.

［9］ Min JY, Sullivan MF, Yang YK, et al. Significant improvement of heart function by cotransplantation of human mesenchymal stem cells and fetal cardiomyocytes in postinfarcted pigs［J］. Ann Thorac Surg, 2002,74:1568-1575.

［10］ Thompson RB, Emani SM, Davis BH, et al.Comparison of intracardiac cell transplantation: autologous skeletal myoblasts versus bone marrow cells［J］. Circulation,2003,108（Suppl 1）:II264-II271.

［11］ Taylor DA. Cellular cardiomyoplasty with autologous skeletal myoblasts for ischemic heart disease［J］. Curr Control Trials Cardiovasc Med,2001, 2: 208-210.

［12］ Siminiak T, Kalawski R, Fiszer D, et al. Transplantation of autologous skeletal myoblasts in the treatment of patients with postinfarction heart failure［J］. Circulation, 2002,106 (suppl II)（Abstract）: II-636.

［13］ 宋学立. 大鼠心肌细胞的培养纯化及其在药理毒理学中的应用［J］. 实用医药杂志,2002,19（12）:950-951.

备注/Memo

备注/Memo:
收稿日期：2004-11-08.
更新日期/Last Update: 2010-01-05