我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

动员自体骨髓干细胞对急性心肌梗死大鼠心功能的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第4期
页码:
326-329
栏目:
基础研究
出版日期:
2005-09-05

文章信息/Info

Title:
The effect of bone marrow stem cells mobilization on cardiac performance of acute myocardial infarction rats
作者:
武峰1; 王海昌1; 王跃民2; 李伟杰1; 张荣庆1; 秦涛1; 李虎1
第四军医大学：1.西京医院心血管内科，2.基础部生理学教研室，陕西 西安 710032
Author(s):
WU Feng1; WANG Haichang1; WANG Yuemin2;  LI Weijie1;  ZHANG Rongqing1; QIN Tao1; LI Hu1
1. Department of Cardiovasology,Xijing Hospital, 2.Department of Physiology,Fourth Military Medical University,Xi'an，Shaanxi 710033,China
关键词:
骨髓干细胞; 自体动员; 心肌梗死; 急性
Keywords:
bone marrow stem cells;  mobilization;  myocardial infarction; acute
分类号:
R542.22
DOI:
-
文献标识码:
A
摘要:
目的 探讨联合应用粒细胞集落刺激因子（GCSF）和干细胞因子（SCF）动员心肌梗死大鼠的骨髓干细胞并观察对心功能的影响及探讨其可能机制。方法 雄性Wistar大鼠40只经结扎冠状动脉前降支制作心肌梗死模型后随机分为两组。①动员组：皮下注射rhCSF和rhSCF。②对照组：皮下注射等量生理盐水。28 d后通过测定血流动力学观察大鼠心功能变化，通过伊文氏蓝TTC染色方法观察心肌梗死范围的变化。通过HE染色方法、免疫荧光标记染色和双重免疫荧光标记染色观察心肌梗死范围内心肌纤维化、血管和心肌再生的变化。结果 动员组心肌组织梗死范围内成纤维细胞增生程度轻，新生血管和心肌细胞的特异蛋白染色阳性，与对照组比较，动员组左室收缩压显著增大（P<0.05），舒张末压显著减小（P<0.05）,左室压上升/下降变化最大速率显著增快（P<0.05）。动员组的缺血范围和心肌梗死的范围比对照组均有所缩小（P<0.05）。结论 GCSF和SCF动员骨髓干细胞可以改善心肌梗死后大鼠的心功能。
Abstract:
AIM To explore the effect and the possible mechanism of GCSF and SCF on cardiac function by mobilizing the bone marrow stem cells in acute myocardial infarction rats. METHODS Myocardial infarction of left ventricle was induced in male Wistar rats by ligation of left coronary artery. The rats（n=40）were randomly divided into 2 groups: ①The mobilized group receiving hypoderm injection of rhGCSF and rhSCF, and ② The control group receiving hypoderm injection of an equivalent amount of normal saline. After 28 days, heart function was examined and hearts were harvested for HE stain,immunofluorescence and double immunofluorescence. Myocardial ischemic size and infarct size were determined by dual staining with Evan's blue dye and triphenylterazolium chloride. RESULTS Compared with the control group, fibrosis was less in the infarct zone of the mobilized group. Regenerative vasculars and myocytes specific proteins were stained positively. Left ventricular systolic pressure,left ventricular enddiastolic pressure and left ventricular pressure decay ±dp/dtmax of the mobilized group were higher than those of the contral group (P<0.05).There was statistical differences between the two groups. Myocardial ischemic size and infarct size of the mobilized group［(41±7)% and (43±6)%］ were statistically smaller than those of contral group；［(53±8)% and (55±10)%］(P<0.05). CONCLUSION By using GCSF and SCF , the heart function of acute myocardial ischemic rats is improved.

参考文献/References

［1］ Jackson KA, Mi T,Goodell MA,et al.Hematopoietic potential of stem cells isolated from murine skeletal muscle［J］. Proc Natl Acad Sci USA,1999，96:14482-14486.

［2］ Tomita S,Li RK,Weisel RD, et al. Autologous transplantation of bone marrow cells improves damaged heart function［J］. Circulation,1999，100(19 suppl):Ⅱ247-Ⅱ256.

［3］ Anversa P,NadalGinar B. Myocyte renewal and ventricular remodelling［J］. Nature,2002，415：240-243.

［4］Helmuth L.Stem cells hear call of injured tissue［J］. Science,2000，290:1479-1481.

［5］ 高峰,施东伟,王晓明，等. 极化液对缺血/再灌大鼠心肌细胞死亡及心脏功能的影响：胰岛素的关键作用［J］. 中华内科杂志,2003，42(3):148-152.

［6］ Milavelz JJ,Raya TE,Johnson CS,et al. Survival after myocardial infarction in rats: Captopril versus iosartan［J］. JMCC,1996;27:714.

［7］ Gao F，Yue CL，Shi DW, et al. p38 MARK inhibition reduces myocardial reperfusion injury via inhibition of endothelial adhesion molecule expression and blockade of PMN accumulation［J］. Cardiovasc Res,2002,53:414-422.

［8］ Wright DE,Amy JW,Anjali PG,et al. Physiological migration of hematopoietic stem and progenitor cells［J］. Science,2001，294:1933-1936.

［9］ Strauer BE, Kornowski R. Stem cell therapy in perspective［J］. Circulation,2003，107:929-934.

［10］ Kocher AA, Schuster MD, Szaboles ML, et al. Neovascularization of ischemic myocardium by human bonemarrowderived angioblasts prevents cardiomyocyte apoptosis reduces remodeling and improve cardiac function［J］. Nat Med,2001,7(4):430-436.

［11］ Conget PA,Minguell JJ. Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells［J］ . J Cell physiol,1999,181(1):67-73.

［12］ Orlic D,Kajstura J, Chimenti S, et al. Bone marrow cells regenerate infarcted myocardium［J］. Nature,2001,410:701-705.

备注/Memo

备注/Memo:
收稿日期：2004-11-04.
更新日期/Last Update: 2010-01-05