利用内皮祖细胞制备组织工程血管的实验研究(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2005年第5期
- 页码:
- 431-433,437
- 栏目:
- 基础研究
- 出版日期:
- 2005-10-05
文章信息/Info
- Title:
- Effects and mechanisms of matrine on myocardium fibrosis in spontaneously hypertensive rat
- Author(s):
- ZHU Xinye; GAO Dengfeng; NIU Xiaolin; Wang ZiYing
- Second Hospital of Xi′an Jiaotong University, 1.Department of Emergency, 2. Department of Cardiology, Xi′an, Shaanxi 710004, China; 3.The Key Laboratory of Environment and Genes Related Diseases, Xi′an, Shaanxi 710061,China
- Keywords:
- hypertension; Myocardium; fibrosis; matrine
- 分类号:
- R318.11
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 观察内皮祖细胞（endothelial progenitor cells ，EPCs）在去细胞血管段上的再内皮化效果，探讨其组织工程学应用前景。方法 采用贴壁选择法培养人外周血EPCs，VEGF扩增分化，流式细胞仪分析培养细胞CD34、VECadherin的表达，DiIacLDL 吞噬试验、Ⅷ因子相关抗原免疫组化及细胞形态观察证实培养细胞的内皮属性。采用球囊损伤法制备去内膜兔腹主动脉段，去内皮兔腹主动脉段体外培养3周使之去细胞，与人外周血EPCs共孵育制备再内皮化异种去细胞血管段。结果 体外成功培养出人外周血内皮祖细胞，EPCs与去细胞血管段共培育使之再内皮化，形成新内膜。结论 体外培养EPCs可制备再内皮化的异种去细胞血管段，提供了一种制备组织工程血管的新方法。
- Abstract:
- AIM To study the effects of Matrine on myocardial fibrosis in spontaneously hypertensive rat(SHR). METHODS SHRs of 8weekold were randomly divided into 3 groups:SHR(water,10 ml/kg·d）,Mat (matrine, 100 mg/kg·d), Bena (Benapril, 10 mg/kg·d),each group consisting of 6 rats.And 6 normal WKY rats were used as negative control. One day before and after 8week treatments, we measured the SBP ,determined the LVMI; We also observed the change of structure and shape in the myocardial cells with masson, observed the expression of ColⅢ with immunohistochemistry; we determined the levels of serum LN and PCⅢ and the level of serum TGFβ1 with radioimmunoassay technique. RESLUTS ①Compared with WKY rats,the SBP,LVMI of SHR Were significantly increased. After the treatment of Benapril, the SBP,LVMI of SHR were significantly decreased. After the treatment of Matrine, the SBP, LVMI had no significant change. ②Compared with WKY rats, the myocardial ColⅢ, serum PCⅢ, LN were significantly increased.After treatment of Benapril, the myocardial ColⅢ, serum PCⅢ,LN were significantly decreased. After the treatment of Matine, myocardial ColⅢ,serum PCⅢ,LN were significantly decreased. ③Compared with WKY,the serum level of TGFβ1 in SHR were significant increased. After treatment of Benapril, the serum level of TGFβ1 in SHR were significantly decreased. After the treatment of Matine serum level of TGFβ1 in SHR were significantly decreased. CONCLUSION Although Matrine can not decrease blood pressure and inhibit myocardial hypertrophy, it effectively decreases myocardial ColⅢ, serum PCⅢ,LN by decreasing serum TGFβ1.
参考文献/References
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备注/Memo
- 备注/Memo:
- 收稿日期：2004-12-16.基金项目：浙江省自然科学基金资助（No.M303648）
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