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|本期目录/Table of Contents|

曲尼司特抑制大鼠胸主动脉球囊损伤后再狭窄(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第3期
页码:
289-292
栏目:
基础研究
出版日期:
2006-06-25

文章信息/Info

Title:
Tranilast in preventing restenosis after rat thoracic aorta balloon injury
作者:
徐超; 江洪; 姚园; 王腾
武汉大学人民医院心内科， 湖北 武汉 430060
Author(s):
XU Chao;  JIANG Hong;  YAO Yuan;  WANG Teng
Department of Cardiology, Renmin Hospital, Wuhan University, Wuhan, Hubei 430060, China
关键词:
曲尼司特; 细胞周期; 周期素依赖激酶抑制因子; 新生内膜
Keywords:
tranilast;  cell cycle ;  p27 ;  neointima
分类号:
R543.1
DOI:
-
文献标识码:
A
摘要:
目的 探讨曲尼司特对大鼠球囊损伤血管新生内膜增殖反应的干预作用及对细胞周期调控因子表达的影响。方法 术后14 d处死动物，HE染色法观察形态学变化；用免疫组化方法鉴定各组p27的表达；另外用流式细胞术测定平滑肌细胞各期（G1期, S期, G2/M期）百分比。结果 ① 假手术组无新生内膜形成,模型组内膜增生显著,曲尼司特组内膜增生减轻［(0.32±0.05） mm2 vs （0.14±0.01） mm2, P<0.01］。 ② 免疫组织化学染色显示，模型组新生内膜处p27低表达, 曲尼司特组新生内膜p27表达强烈（20.8±1.7 vs 10.5±0.7,P<0.01）。③ 流式细胞术显示，与模型组相比，曲尼司特组平滑肌细胞处于增殖期的细胞比例明显降低［(27.5±1.1）% vs （13.5±1.5）%， P<0.01］。结论 曲尼司特能抑制血管平滑肌细胞增殖,其抗增殖机制可能与细胞周期调控和上调p27相关。
Abstract:
AIM To investigate the influence of tranilast on cell cycle modulatory factors and proliferation response of vascular smooth muscle cell after balloon angioplasty. METHODS Aortic tissues were harvested at day 14 after injury and HE staining and immunohistochemistry staining were used to measure the protein expressions of cell cycle modulatory factors p27. Flow Cytometry (FCM) was applied to determine the cell cycle of the neointima. RESULTS ① Neointima formation was apparent in the arteries from the operation group and was reduced much more in the tranilast group ［(0.32±0.05) mm2 vs (0.14±0.01) mm2, P<0.01)］. ②Immunohistochemistry staining revealed that p27 protein expressions were low in the neointima of the model group and were significantly increased by tranilast (20.8±1.7 vs 10.5±0.7, P<0.01). ③ FCM showed that in the model group, the percentage of S phase of smooth muscle cells was high but declined in the tranilast group ［(27.5±1.1)% vs (13.5±1.5)%, P<0.01］. CONCLUSION Tranilast surpresses neointima formation after artery injury by up-regulation of p27.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2005-07-26.通讯作者：江洪，教授，主要从事心血管介入治疗研究 Tel：（027）880419198072 作者简介：徐超 ，硕士生 Tel：（027）88041919 Email：xuchao108@yeah.net
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