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放射性PDGFR-β反义寡核苷酸对血管平滑肌细胞增殖和凋亡的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第4期
页码:
388-391
栏目:
基础研究
出版日期:
2006-08-25

文章信息/Info

Title:
Effects of 32P labeled PDGFR-β-antisense oligonucleotide on proliferation and apoptosis of rat vascular smooth muscle cells
作者:
王云雅;  汪静;  邓敬兰;  李国权;  林国成;  顾春虎
第四军医大学西京医院核医学科， 陕西 西安 710032
Author(s):
WANG Yun-ya;  WANG Jing;  DENG Jing-lan;  LI Guo-quan;  LIN Guo-cheng;  GU Chun-hu
Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University, Xi′an, Shaanxi 710032, China
关键词:
反义寡核苷酸; 再狭窄; 细胞; 平滑肌; 血管; 血小板衍化生长因子
Keywords:
antisense oligonucleotide; restenosis; cell; smooth muscle; vascular; platelet derived growth factor; receptor
分类号:
R331.3;R329.2
DOI:
-
文献标识码:
A
摘要:
目的 研究32P标记血小板衍化生长因子β受体（PDGFR-β）的反义寡核苷酸（AON）对血管平滑肌细胞(VSMC)增殖的影响,为抑制血管术后再狭窄的形成提供可能的干预。方法 体外进行大鼠VSMC的培养，以5～10代细胞为实验对象，人工合成PDGFR-β-AON，并进行32P标记, 按实验目的分组。MTT法分析细胞增殖活力，以流式细胞仪测定细胞周期，用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL）检测细胞凋亡。结果 32P标记PDGFR-β-AON作用后，VSMC的增殖强度明显弱于空白对照组（P<0.01），而处于DNA合成前期(G0/G1)细胞百分比高于空白对照组（P<0.01）； 32P标记AON组细胞凋亡率明显高于空白组（P<0.01）。结论 32P-PDGFR-β-AON可以诱导VSMC凋亡，抑制其增殖。
Abstract:
AIM To study the effects of 32P labeled PDGFR-β-(paletelet derived growth factor receptorβ) antisense oligonucleotides (AON) on the proliferation and apoptosis of cultured rat vascular smooth muscle cells (VSMC) and to propose a possible approach to the prevention of restenosis. METHODS Cultured rat VSMC proliferation model was established in vitro and cells of 5-10 passages were used for the experiment. Two different PDGFRβ oligonucelotides were synthesized and labeled with 9 250 Bq 32P (antisense, 5′TAT CAC TCC TGG AAG CCC3′.sense, 5′GGG CTT CCA GGA GTG ATA3′). MTT assay and flow cytometry were used to determine the effects of 32P labeled PDGFRβ AON on the proliferation of VSMC. Apoptotic VSMC was detected and assayed by terminal deoxynucleotidyl transferasemediated dUTP nickended labeled (TUNEL). RESULTS The MTT assay showed that the proliferation of VSMC could be inhibited by 32PPDGFR-β-AON (P<0.01). The percentage of quiescent cells (G0/G1) of 32PPDGFRβ AON group was significantly increased compared with those of control group (P<0.01). Compared with that of control group, the number of apoptotic cells of 32PPDGFRβ AON group was obviously higher at 48 hours. CONCLUSION 32PPDGFRβ AON can induce the apoptosis and inhibit the proliferation of cultured VSMC.

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备注/Memo

备注/Memo:
收稿日期：2005-06-10.作者简介：王云雅,技师 Tel：（029）84771052 Email：yunyaw@126.com
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