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灯盏花素对大鼠心肌缺血再灌注损伤诱导细胞凋亡的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第5期
页码:
506-508
栏目:
基础研究
出版日期:
2006-10-25

文章信息/Info

Title:
Effect of Breviscapine on myocyte apoptosis in myocardial ischemiareperfusion rat model
作者:
吴岩松;  李树青; 赵振梅
锦州医学院附属第一医院心内科, 辽宁 锦州 121000
Author(s):
WU Yan-song;  LI Shu-qing;  ZHAO Zhen-mei
Department of Cardiology First Affiliated Hospital, Jinzhou Medical College, Jinzhou,Liaoning 121000, China
关键词:
灯盏花素; 心肌再灌注损伤; 细胞凋亡; 基因表达
Keywords:
Breviscapine; myocardial reperfusion injury; apoptosis; gene expression
分类号:
R541.4
DOI:
-
文献标识码:
A
摘要:
目的 观察灯盏花素(BRE)对大鼠心肌缺血再灌注(I/R)诱导细胞凋亡及凋亡相关基因Bcl2、Bax表达的影响，并对其作用机制进行初步探讨。方法 30只SD大鼠随机分为3组(n=10)：①假手术(Sham)组：前降支穿线不结扎，观察1.5 h；②缺血再灌注(I/R)组：结扎前降支30 min，再灌注前5 min生理盐水(4 mg/kg) iv，再灌注1 h；③BRE组：再灌注前5 min应用BRE(4 mg/kg)iv，余同I/R组。常规方法检测丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性，免疫组化法测定Bcl2、Bax蛋白的表达，通过细胞图像分析系统测量细胞阳性染色面积百分率，应用透射电镜观察心肌超微结构的变化。结果 同I/R组相比，BRE组心肌组织MDA含量显著降低［(7.56±1.67)μmol/g vs (16.58±2.59)μmol/g，P<0.05］，SOD活性显著升高［(2.37±0.22) mkat/g vs （1.38±0.14） mkat/g，P<0.05］，Bax蛋白表达显著降低(5.02±1.01 vs 18.59±5.68，P<0.01)，而Bcl2蛋白表达显著增高(17.48±4.72 vs 7.32±2.05，P<0.01)，Bax/Bcl2显著降低(0.29±0.57 vs 3.32±0.10，P<0.01)，细胞阳性染色面积百分率显著减少(0.42±0.06 vs 13.84±1.97，P<0.01)，细胞超微结构损伤明显减轻。结论 BRE通过调控Bax/Bcl2表达抑制心肌I/R诱导的细胞凋亡，对缺血再灌注心肌具有保护作用。
Abstract:
AIM To observe the effect of Breviscapine on myocyte apoptosis and expression of Bcl2 and Bax in myocardial ischemiareperfusion rat model and to investigate the preliminary mechanism of Breviscapine. METHODS Thirty SpragueDawley rats were randomly divided into three groups (n=10): ①Sham operation group; ②Ischemiareperfusion (I/R) group, in which the left coronary artery was occluded for 30 min followed by 1 hour reperfusion, and normal saline (4mg/kg) was given intravenously 5 min before the initiation of reperfusion; ③ Breviscapine group, in which treatment similar to that of I/R group was given except in place of normal saline Breviscapine (4 mg/kg) was given intravenously 5 min before the initiation of reperfusion. Routine method was used to detect the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA). The expressions of Bcl2 and Bax protein were measured by immunohistochemical technique. Image processing system was used to quantitatively dispose the positive metric substance of immunohistochemistry. The ultrastucutural change of the myocardium was observed under electorn microscope. RESULTS Compared with those in I/R group, the myocardial MDA contents decreased significantly ［(7.56±1.67)μmol/g vs (16.58±2.59)μmol/g, P<0.05］ while the myocardial SOD activities increased significantly in Breviscapine group ［(2.37±0.22) mkat/g vs (1.38±0.14)mkat/g, P<0.05］. In Breviscapine group, the protein expression of Bax gene obviously decreased (5.02±1.01 vs 18.59±5.68, P<0.01), the protein expression of Bcl2 gene obviously increased (17.48±4.72 vs 7.32±2.05, P<0.01), the number of Bax/Bcl2 was obviously decreased (0.29±0.57 vs 3.32±0.10, P<0.01), and the positive metric substance of immunohistochemistry decreased markedly (0.42±0.06 vs 13.84±1.97, P<0.01). The pathological changes of myocardial ultrastructure in Breviscapine group showed relatively attenuated damage compared with that in I/R group. CONCLUSION Breviscapine inhibits the apoptosis induced by myocardial I/R by modulating the expression of Bax and Bcl2 protein.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2005-10-25.通讯作者：李树青，教授，主要从事冠心病防治研究 Tel:(0416)4197512 Email:lishuqing1949@126.com 作者简介：吴岩松，硕士生Email：jzyxywys@yahoo.com.cn
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