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环氧氯丙烷对组织工程瓣去细胞支架材料理化性质的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第5期
页码:
524-527
栏目:
基础研究
出版日期:
2006-10-25

文章信息/Info

Title:
The effect of epoxy chloropropane treatment on the physicochemical properties of the acellular stent materials of tissueengineering bioprostheses
作者:
易蔚1; 魏旭峰2; 俞世强2
第四军医大学西京医院：1.移植外科中心, 2.全军心血管病研究所心血管外科，陕西 西安 710032
Author(s):
YI Wei1;  WEI Xu-feng2;  YU Shi-qiang2
1.Department of Transplantation; 2.Department of Cardiovascular Surgery, Institute of Cardiology, Xijing Hospital, the Forth Military Medical University, Xi′an, Shannxi, 710032, China
关键词:
组织工程瓣; 支架材料; 环氧氯丙烷; 理化性质
Keywords:
tissueengineering bioprostheses;  stent materials;  epoxy chloropropane;  physicochemical property
分类号:
R542.5
DOI:
-
文献标识码:
A
摘要:
目的 研究环氧氯丙烷（EC）处理对组织工程心脏瓣膜去细胞支架材料理化性质的影响。方法 对EC处理的去细胞组织工程猪瓣支架材料进行胶原酶消化实验，茚三酮实验，血浆蛋白吸附率和血小板黏附率实验，与戊二醛（GA）处理的及未经处理的去细胞猪瓣支架材料进行对照研究，评价EC处理对生物材料理化性质的影响。结果 胶原蛋白经EC或GA处理后，抗消化能力显著增加(P<0.01)；GA处理后的胶原蛋白再经EC处理后，抗消化能力进一步增加(P<0.01)。胶原蛋白经EC和GA处理后，茚三酮值均明显下降(P<0.01)，GA处理后的胶原蛋白经EC处理后，茚三酮值进一步下降(P<0.01)。EC和GA处理胶原蛋白的交联度增加，EC处理能使GA处理的胶原蛋白交联度进一步增加(P<0.01)。EC处理的组织工程瓣膜去细胞支架材料血浆中蛋白质吸附率和血小板黏附率均降低。结论 EC处理能够进一步改善组织工程瓣膜去细胞支架材料的理化性质，有利于提高血液相容性和减缓钙化，是组织工程瓣生物支架材料很好的处理方法。
Abstract:
AIM This study was carried out to define the effect of epoxy chloropropane treatment on the physicochemical properties of the acellular stent materials of tissueengineering bioprostheses (ASM). METHODS The physicochemical properties of ASM treated with EC and treated with both EC and GA was evaluated by measuring plasma protein adsorption, platelet adhesion, ninhydrin reaction, and digestion resistance to collagenase enzyme. The ASM treated with GA and treated without any treatment was tested as control. RESULTS Resistance to collagenase digestion revealed that collagen of the EC treated ASM had greater resistance to enzyme digestion than with GA treatment and the untreated ASM (P<0.01). Ninhydrin assay showed that the crosslinking of collagen of the EC treated ASM increased greatly compared with GA treatment and the untreated ASM (P<0.01). Both the plasma protein adsorption and platelet adhesion of ASM after EC treatment reduced greatly compared to the GA treated ASM (P<0.0118). And after GA pretreated, EC treatment lowered both the plasma protein adsorption and platelet adhesion of the ASM as compared to GA treatment(P<0.01). CONCLUSION EC treatment of the ASM enhances crosslinking of the collagen and shows better physicochemical properties, so it mitigates calcification.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2006-07-10.基金项目：国家自然科学基金项目资助（No.30471716） 通讯作者：俞世强，主任医师,主要从事微创心外及瓣膜外科的研究 Tel:(029)84775576 Email:ysq2002@21cn.com 作者简介：易蔚，博士生 Tel: (029)84775620 Email: penny_yi7@hotmail.com
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