文章摘要

文章信息/Info

Title:: Cell extraction from porcine aortic valve

作者:: 顾春虎¹; 王云雅²; 易定华¹; 张近宝¹; 刘维永¹; 刘洋¹; 第四军医大学西京医院:1.心脏外科,2.核医学科,陕西西安 710032

Author(s):: GU Chun-hu¹; WANG Yun-ya²; YI Ding-hua¹; ZHANG Jin-bao¹; LIU Wei-yong¹; LIU Yang¹; 1.Department of Cardiovascular Surgery, 2.Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University, Xi′an,Shaanxi 710032, China

关键词:: 瓣膜; 胰蛋白酶; 组织工程; 脱细胞基质

Keywords:: valve; trypsin; tissue engineering; acellular tissue matrix

分类号:: R33;Q813.5

DOI:: -

文献标识码:: A

摘要:: 目的完全去除猪主动脉瓣细胞，取得良好的去细胞支架材料。方法用5 g/L胰酶对标本中的某些基质和细胞加以消化后，再应用不同去垢剂（十二烷基硫酸钠、脱氧胆脂酸钠、Triton X-100）加以洗脱，结合溶液渗透压改变等去除猪主动脉瓣细胞；标本进行大体、光镜、电镜观察和热皱缩温度的检测。结果胆脂酸钠无法完全脱除猪主动脉瓣细胞，十二烷基硫酸钠和曲拉通均可完全去除猪主动脉瓣细胞，Triton X-100组的主动脉瓣胶原纤维和弹性纤维得以较好保持。结论 Triton X-100可以成功脱除猪主动脉瓣细胞，为组织工程瓣膜的研究提供材料。

Abstract:: AIM To explore method to remove cellular components from porcine aortic valve, and obtain decellularised scaffolds for tissue engineering of heart valve. METHODS To remove all cells from porcine aortic valve, samples were digested in a 0.1% trypsin solution, followed by multistep detergentenzymatic process with both hypotonic and hypertonic solutions and ionic detergent. Different detergents (Sodium dodecyl sulfate and sodium deoxycholate, Triton X-100) and enzyme (trypsin) extraction were applied. Specimens were observed grossly and the changes of shrinkage temperature were also studied. Haematoxylineosin was performed to confirm the removal of cells and transmission electron microscope was used to observe the integrity of collagen and elastin. RESULTS The cells of porcine aortic valve were removed effectively by Triton X-100 and sodium dodecyl sulfate. Sodium deoxycholate could not effectively remove the cells from the root of aortic valve. The major structural components and shrinkage temperature of decellularised porcine aortic valve by Triton X-100 were well maintained. CONCLUSION Acellular tissue matrix (ACTM) of porcine aortic valve can be successfully obtained through this procedure by Triton X-100.

参考文献/References

［1］顾春虎,刘维永,张近宝，等. 不同试剂脱除猪胸主动脉壁细胞的对比研究［J］.心脏杂志， 2005，17（3）：207-209.

［2］ Zimmermann WH, Eschenhagen T. Tissue engineering of aortic heart valves ［J］. Cardiovasc Res, 2003, 60（3）:460-462．

［3］Booth C,Korossis SA,Wilcox HE,et al.Tissue engineering of cardiac valve prostheses Ⅰ：development and histological characterization of an acellular porcine scaffold［J］. J Heart Valve Dis ,2002,11（4）:457-462.

［4］ Kim WG, Park JK, Lee WY. Tissueengineered heart valve leaflets: an effective method of obtaining acellularized valve xenografts［J］. Int J Artif Organs ,2002, 25(8):791-797.

［5］ Korossis SA, Booth C,Wilcox HE,et al. Tissue engineering of cardiac valve prosthesesⅡ：biomechanical characterization of decellualrized porcine aortic heart valves ［J］. J Heart Valve Dis, 2002,11（4）:463-471.

［6］ Wilson GJ,courtman DW,Klement P,et al. Acellular matrix:a biomaterial approach for coronary artery bypass and heart valve replacement［J］. Ann thorac Surg,1995,60（2 suppl):S353-S358.

［7］韩雪峰，杨大平，郭铁芳. Triton X100对制备脱细胞血管基质影响的实验研究［J］.中华外科杂志, 2002， 40(1)：27-29.

［8］ Hoerstrup SP, Sodisn R, Daebritz S, et al．Functional living trileaflet heartvalves grown in vitro［J］. Circulation，2000,102(suppl III):III44-III49.

［9］ Teebken OE, Bader A. Steinhoff G,et al. Tissue engineering of vascular grafts: human cell seeding of decellularised porcine matrix［J］. Eur J Vasc Endovasc Surg, 2000,19(4): 381-386.

［10］Sodian R, Hoerstrup SP, Sperling JS,et al. Tissue engineering of heart valves:in vitro experience［J］. Ann Thorac Surg, 2000,70(1):140-144.

［11］俞世强，赵东锷，汪钢，等. 应用胶原膜构建组织工程心脏瓣膜的初步研究［J］.心脏杂志, 2001,13（5）：372-376.

我们的网站为什么显示成这样？

猪主动脉瓣去细胞基质的制备(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

文章信息/Info

参考文献/References

备注/Memo

常用功能

导航/Navigate

工具/Tools

统计/Statistics