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|本期目录/Table of Contents|

重组腺相关病毒介导的CD151基因转移对大鼠缺血心肌血管密度的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第1期
页码:
1-4
栏目:
基础研究
出版日期:
2007-01-01

文章信息/Info

Title:
Influence of rAAVmediated CD151 gene transfer on capillary density in rat myocardial infarction model
作者:
郑振中;  刘正湘
华中科技大学同济医学院附属同济医院心血管内科，湖北 武汉 430030
Author(s):
ZHENG Zhenzhong;  LIU Zhengxiang
Department of Cardiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China
关键词:
CD151; 心肌梗塞; 毛细血管密度
Keywords:
CD151;  myocardial infarction;  capillary density
分类号:
R542.2
DOI:
-
文献标识码:
A
摘要:
目的 通过将携带人CD151基因的重组腺病毒相关病毒(rAAV) 载体注入缺血心肌， 观察CD151基因对缺血心肌血管密度的影响。方法 结扎冠状动脉前降支制作大鼠急性心肌梗死模型,将rAAVCD151注入缺血心肌处。4周后取注射部位心肌,用逆转录聚合酶链式反应(RTPCR)检测外源性CD151 mRNA的表达、Western blot检测CD151的表达、Ⅷ因子相关抗原免疫组化染色并计数注射部位心肌毛细血管数目，评价外源CD151对毛细血管密度的影响。结果 术后4周, CD151组心肌组织检测到外源性CD151 mRNA的表达, 假手术组、缺血对照组、GFP组未检测到外源性CD151 mRNA的表达，且CD151组心肌组织CD151蛋白表达水平高于假手术组、缺血对照组、GFP组(P<0.01); CD151组血管密度与缺血对照组、GFP组比较显著增加(P<0.01)。结论 rAAVCD151可以有效地转染大鼠心肌组织,促进缺血心肌的血管生成。
Abstract:
AIM To investigate the influence of recombinant adenoassociated virus mediated CD151 (rAAVCD151) gene on the capillary density in a rat myocardial infarction model. METHODS Rat myocardial infarction model was formed by ligation of the anterior descending coronary artery and rAAVCD151 was injected into the ischemic myocardium. At 4 weeks after coronary artery ligation,human CD151 mRNA was detected using RTPCR, Western blot analysis for CD151 was performed, and the measurement of capillary density was evaluated using immunostaining for von Willebrand factor. RESULTS Human CD151 mRNA was detected in rAAVCD151 group. Compared with the rAAVGFP group and control group, CD151 gene delivery significantly increased the expression of CD151 (P<0.01) and capillary density after myocardial infarction in ischemic myocardium (P<0.01). CONCLUSION rAAVCD151 increases the capillary density after myocardial infarction in rats.

参考文献/References

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［2］ Lan RF, Liu ZX, Liu XC，et al. CD151 promotes neovascularization and improves blood perfusion in a rat hindlimb ischemia model［J］. J Endovasc Ther, 2005 ,12(4):469-478.

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［8］ Sasaki H, Ray PS, Zhu L, et al. Hypoxia/reoxygenation promotes myocardial angiogenesis via an NF kappa Bdependent mechanism in a rat model of chronic myocardial infarction［J］. J Mol Cell Cardiol，2001，33(2):283-294.

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备注/Memo

备注/Memo:
收稿日期：2006-06-29.刘正湘，教授，主要从事冠心病防治和介入治疗的研究 Email:ZXliu@tjh.tjmu.edu.cn 作者简介：郑振中，博士，主治医师 Email:greateful@163.com
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