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Title:: Number and activity change of cardiac endothelial progenitor cells in unstable angina pectoris patients

作者:: 刘兰¹; 2; 黄岚¹; 陈剑飞¹; 晋军¹; 宋耀明¹; 李爱民¹; 于学军¹; 覃军¹; 耿昭华¹; 赵刚¹; 1.第三军医大学新桥医院全军心血管内科研究所，重庆 400037;2.成都军区机关医院, 四川成都 610011

Author(s):: LIU Lan; HUANG Lan; CHEN Jianfei; JIN Jun; SONG Yaoming; LI Aimin; YU Xuejun; QIN Jun; GENG Zhaohua; ZHAO Gang; Department of Cardiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China

关键词:: 心绞痛; 不稳定型; 内皮祖细胞; 冠脉循环

Keywords:: Angina; unstable ; endothelial progenitor cells; coronary circulation

分类号:: R541.4

DOI:: -

文献标识码:: A

摘要:: 目的观察不稳定型心绞痛(UA)患者外周循环及心脏局部内皮祖细胞(EPCs)数量及功能的改变。方法 27例UA患者均经冠状动脉造影(CAG)证实有明显冠状动脉狭窄，20例对照组患者经临床检查和CAG排除冠心病。密度梯度离心法从外周循环及冠状窦获取单个核细胞，将其接种在人纤维连接蛋白包被培养板，培养14 d后贴壁细胞进行细胞化学分析。激光共聚焦显微镜鉴定FITCUEAI和DiIacLDL双染色阳性细胞为正在分化的EPCs。采用二苯基四氮唑嗅盐比色法、改良的Boyden小室和黏附能力测定实验观察EPCs的增殖能力、迁移能力和黏附能力。结果 ① 与对照组比较，UA患者外周循环及心脏局部EPCs数量均明显减少，EPCs的黏附、迁移及增殖能力也显著受损（P<0.01)。② UA患者冠状窦EPCs的数量增加，黏附和增殖能力较外周循环有所下降（P<0.05），迁移能力无明显差别。结论 UA患者心脏局部EPCs的数量下降和功能降低，提示冠状动脉内皮损伤后修复能力低下，可能与病情加重有关。

Abstract:: AIM To investigate the number and activity changes of endothelial progenitor cells (EPCs) from peripheral circulation and the coronary sinus in unstable angina pectoris patients (UA). METHODS Twentyseven patients with unstable angina and 20 patients excluding coronary heart disease (control) were studied. Total mononuclear cells (MNCs) were isolated from peripheral blood and coronary sinus blood by Ficoll density gradient centrifugation and then were plated on humanfibronectincoated dishes. After 7 days of culture, EPCs were characterized as adherent cells by double staining FITCUEAI binding and DiIacLDL uptake under a laser scanning confocal fluorescence microscopy. EPCs proliferation and migration were determined with MTT assay and modified Boyden chamber assay, respectively. EPCs adhesive assay was performed by replanting MNCs on humanfibronectincoated plates and then counting the adherent cells. RESULTS ①The number of EPCs was significantly reduced in UA patients compared with that in control group (P<0.01). EPCs’ proliferative, migratory and adhesive capacity were also impaired (P<0.01) in unstable angina patients.②The number of EPCs increased but its adhesive and proliferative activity was more severely impaired in coronary sinus blood compared with that in the peripheral blood (P<0.05). These differences were not observed in control group. CONCLUSION The number of EPCs decrease and EPCs dysfunction are inversely associated with the impaired endothelial function in UA patients. These findings imply that the reduced repairing ability following endothelial injury in the circulating EPCs may cause the heart disorder and aggravate the clinical situation.

参考文献/References

［1］ Griese DP, Achatz S, Batzlsperger CA, et al. Vascular gene delivery of anticoagulants by transplantation of retrovirallytransduced endothelial progenitor cells［J］. Cardiovasc Res, 2003,58(2):469-477.

［2］Hill JM, Zalos G, Halcox JP, et al. Circulating endothelial progenitor cells, vascular function, and cardiovascular risk［J］. N Engl J Med, 2003,348(7):593-600.

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［4］中华医学会心血管病学分会，中华心血管病杂志编辑委员会. 不稳定性心绞痛诊断和治疗建议［J］. 中华心血管病杂志, 2000,28(6): 409-412.

［5］Vasa M, Fichtlscherer S, Aicher A, et al. Number and migratory activity of circulating endothelial progenitor cells inversely correlate with risk factors for coronary artery disease［J］. Circ Res, 2001,89(1):e1-e7.

［6］Kalka C, Masuda H, Takahashi T, et al. Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization［J］. Proc Natl Acad Sci U S A, 2000,97(7):3422-3427.

［7］Suzuki T, Nishida M, Futami S, et al. Neoendothelialization after peripheral blood stem cell transplantation in humans: a case report of a Tokaimura nuclear accident victim［J］. Cardiovasc Res, 2003,58(2):487-492.

［8］Murayama T, Tepper OM, Silver M, et al. Determination of bone marrowderived endothelial progenitor cell significance in angiogenic growth factorinduced neovascularization in vivo［J］. Exp Hematol, 2002,30(8):967-972.

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不稳定型心绞痛患者心脏局部内皮祖细胞数量及功能的变化(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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