我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

骨髓间充质干细胞不同移植方法对心肌梗死大鼠心脏的保护作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第3期
页码:
266-268
栏目:
基础研究
出版日期:
2007-06-01

文章信息/Info

Title:
Protective effects of different transplantations of bone marrow mesenchymal stem cells in rat heart of acute myocardial infarction
作者:
王莹1 ; 龚卫琴1 ; 张珊红1; 侯志峰1 ; 王跃民2 ; 李源1
第四军医大学：1.西京医院老年病科，2.生理学教研室,陕西 西安 710032
Author(s):
WANG Ying1;  GONG Weiqin1;  ZHANG Shanhong1;  HOU Zhifeng1;  WANG Yuemin2;  LI Yuan1
1.Department of Geriatrics, Xijing Hospital, 2.Department of Physiology, Fourth Military Medical University, Xi′an 710032，Shaanxi, China
关键词:
骨髓间充质干细胞; 心肌梗塞; 细胞移植; 心脏保护
Keywords:
bone marrow mesenchymal stem cell; myocardial infarction; cell transplantation;  heart protection
分类号:
R541.4
DOI:
-
文献标识码:
A
摘要:
目的 对比研究经尾静脉和心外膜直视注射移植骨髓间充质干细胞（MSCs）对心肌梗死大鼠的心脏保护作用。方法 结扎左冠状动脉前降支制备大鼠心肌梗死模型，采用同种异体MSCs体外分离、纯化、培养和标记，在梗死后2周，A组经尾静脉植入MSCs，B组心外膜直视注射MSCs，C组尾静脉注入等量DMEM培养液为对照。4周后测定心功能，并行免疫组织化学检测。结果 移植组心肌组织中可以观察到DAPI标记细胞存在，免疫组化显示阳性标记细胞αActin肌动蛋白检测阳性。与对照組相比，A和B组左心功能显著改善（P<0.05），A和B组梗死面积明显缩小［分别为(45±2)％，(40±4)％和(41±3)％，P<0.05］。结论 MSCs经静脉移植和心外膜植入都能够归巢至心肌梗死区,对梗死大鼠的心脏都具有保护作用。
Abstract:
AIM To study the protective effect of intravenous and via epicardium transplantation of bone marrow mesenchymal stem cells (MSCs) on rat heart of acute myocardial infarction (AMI). METHODS AMI was created by occluding the left anterior descending artery (LAD) in SD rats. MSCs acquired from healthy rats were isolated, purified, amplificated and labeled with DAPI. Two weeks after MI, the labeled MSCs were transfused into MI rats through caudal vein (group A, n=10) or via epicardium (group B, n=10) while some culture medium was transfused into the control (group C, n=10) group. Four weeks later, heart functions were evaluated and immunohistochemical analysis was performed. RESULTS The labeled cells were viable in the host hearts and immunochemical staining revealed that the engrafted stem cells expressed αActin. Left heart functions and infarct size in group A and group B were more obviously improved compared with those of control group. CONCLUSION Both intravenous and via epicardium transplantation of MSCs can migrate into the infracted areas and have protective effect on the rat heart of AMI.

参考文献/References

［1］ Kessler PD,Byrne BJ.Myoblast cell grafting into heart muscle:cellular biology and potential applications［J］. Annu Rev Physiol,1999，61:219-242．

［2］牛丽丽,曹丰,郑敏,等.同种异体骨髓间充质干细胞移植治疗急性心肌梗死［J］.中华内科杂志,2004,43(3):186-190．

［3］Wang JS,ShumTim D,Galipeau J,et al．Marrow stromal cells for cellular cardiomyoplasty:Feasibility and potential clinical advantages［J］.Thorac Cardiovasc Surg,2000,120(5):999-1005．

［4］高峰,施东伟,王晓明，等.极化液对缺血/再灌注大鼠心肌细胞死亡及心脏功能的影响:胰岛素的关键作用［J］.中华内科杂志,2003,42(3):148-152．

［5］曹丰,贾国良,牛丽丽，等.移植时机对骨髓间充质干细胞修复梗死心肌的影响［J］.第四军医大学学报,2005,26(1):22-27.

［6］Orlic D,Kajstura J,Chimenti S,et al.Mobilized bone marrow cells repair the infarcted heart，improving function and survival［J］. Proc Natl Acad Sci USA,2001,98(18):10344-10349．

［7］Fukuda K.Development of regeneration cardiomyocytes from mesenchaymal stem cells for cardiovascular tissue engineering［J］.Artif Organs,2001,25（3）:187-193.

［8］Tomita S, Mickle DA,Weisel RD,et al.Improved heart function with myogenesis and angiogenesis after autologous porcine bone marrow stromal cell transplantation ［J］. Thorac Cardiovasc Surg,2002,123(6):1132-1140．

备注/Memo

备注/Memo:
收稿日期：2006-07-01.基金项目：陕西省科研发展计划项目资助（No.2002K10-G4）通讯作者：李源,主任医师Email：xjlnbk@fmmu.edu.cn 作者简介：王莹,医师，硕士Email：wy2004@fmmu.edu.cn
更新日期/Last Update: