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Title:: A preliminary study of degradation of collagen treated with epoxy chloropropane

作者:: 李庆新¹; 2; 陈立军²; 魏旭峰¹; 王云¹; 顾继伟¹; 陈俞¹; 易定华¹; 1.第四军医大学西京医院心血管外科,陕西西安710033;2.兰州军区总医院心血管外科,甘肃兰州 730050

Author(s):: LI Qingxin; CHEN Lijun; WEI Xufeng; WANG Yun; GU Jiwei; CHEN Yu; YI Dinghua; Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi′an 710033, China

关键词:: 环氧氯丙烷; 成纤维细胞; 基质金属蛋白酶; 组织工程心脏瓣膜; 胶原

Keywords:: epoxy chloropropane ; fibroblast; matrix metalloproteinases; tissueengineering heart valve; collagen

分类号:: R654.2

DOI:: -

文献标识码:: A

摘要:: 目的通过对人成纤维细胞在环氧氯丙烷（epoxy chloropropane，EC）处理的胶原膜上种植生长的初步观察，了解EC对胶原降解的影响。方法按不同方法处理的胶原膜分组：①单纯胶原组（Con组）;②环氧氯丙烷处理组（EC组）;③戊二醛（glutaraldehyde，GA）处理组（GA组）。将人成纤维细胞（fibroblast，Fb）通过静态种植方法分别种植在不同的胶原膜上，经形态学检测、逆转录聚合酶链反应（RTPCR），观察人成纤维细胞的组织学和超微结构、基质金属蛋白酶 (matrix metalloproteinases ,MMPs) 在mRNA水平上的表达。结果人成纤维细胞在GA组胶原膜上生长较差，MMPs在mRNA水平上的表达增高，在EC组胶原膜上生长良好，MMPs在mRNA水平上的表达减低，两组表达差异显著（P<0.05）。结论 EC处理可以降低胶原的降解。这可能是EC处理生物瓣防钙化的机制之一，同时为EC 处理构建新型防钙化组织工程心脏瓣膜支架提供了实验依据。

Abstract:: AIM To explore the degradation of collagen treated with epoxy chloropropane (EC). METHODS The human fibroblast was seeded onto the collagen membrane treated with EC and glutaraldehyde (GA). The morphological characteristics of the human fibroblast were evaluated by histological and electron microscopic observations. The expression of MMPs of the human fibroblast was determined by reverse transcriptasepolymerase chain reaction (RTPCR). RESULTS Inverted microscope, scanning electron microscopy (SEM) and RTPCR demonstrated that the surface of the collagen membrane treated with EC was confluent, covered with lower MMPs expression on multilayer cells and the surface of the collagen membrane treated with GA was covered with higher MMPs expression on monolayer cells. CONCLUSION The treatment of EC may delay the degradation of collagen, which is one of the mechanisms involved in the mitigating calcification.

参考文献/References

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环氧氯丙烷处理对生物瓣膜胶原降解代谢影响的初步研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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