反义EGFR寡核苷酸对AngⅡ促血管平滑肌细胞增殖效应的影响(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2007年第4期
- 页码:
- 399-401/405
- 栏目:
- 基础研究
- 出版日期:
- 2007-08-01
文章信息/Info
- Title:
- Effect of antisense senquences of epidermal growth factor receptor on proliferation of vascular smooth muscle cells activated by AngⅡ
- Author(s):
- ZHU Yanxia1; WANG Qixian1; DENG Dongbo1; CHEN He1; FANG Qin1; YIN Xiaolong2
- 1.Department of Cardiology, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan, China; 2.Department of Cardiology, Kunming Yan′an Hospital, Kunming 650011, Yunnan, China
- 关键词:
- 反义表皮生长因子受体寡核苷酸; 血管紧张素Ⅱ; 血管平滑肌细胞; 增殖
- Keywords:
- antisense sequences of epidermal growth factor receptor; angiotensin Ⅱ; vascular smooth muscle cells; proliferation
- 分类号:
- R363.2
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 探讨脂质体介导的反义表皮生长因子受体(EGFR)寡核苷酸基因转染对血管紧张素Ⅱ(AngⅡ)促大鼠血管平滑肌细胞(VSMC)增殖的影响。方法 用EGFR寡核苷酸脂质体复合物转染SpragueDawley大鼠VSMC，通过RTPCR、WesternBloting分别检测转染后EGFR mRNA及蛋白的表达情况，用3HTdr掺入法检测经EGFR寡核苷酸转染再用AngⅡ刺激VSMC的增殖情况。结果 反义EGFR寡核苷酸转染大鼠VSMC后，EGFR mRNA及蛋白的表达较正义组及对照组显著减少（P<0.01)；用AngⅡ刺激后，反义组细胞的3HTdr掺入较正义组及对照组显著降低（P<0.01）。结论 脂质体介导的反义EGFR寡核苷酸转染可以减弱AngⅡ的促VSMC增殖效应。
- Abstract:
- AIM To investigate the effect of antisense sequences of epidermal growth factor receptor (EGFR) on the proliferation of vascular smooth muscle cells (VSMCs) activated by angiotensinⅡ (AngⅡ). METHODS SpragueDawley rat VSMCs were transfected with antisense sequences of EGFR and stimulated by AngⅡ. EGFR mRNA and protein in VSMCs were detected by reverse transcription polymerase chain reaction (RTPCR) and WesternBloting. Proliferation of VSMCs was detected by 3HTdr incorporation. RESULTS EGFR mRNA and protein in VSMCs transfected with antisense sequences of EGFR were significantly lower than those transfected with sense sequences of EGFR and control(P<0.01) . The 3HTdr incorporation of VSMCs transfected with antisense senquences of EGFR was markedly lower than that transfected with sense sequences of EGFR and control（P<0.01）. CONCLUSION Antisense sequences of EGFR attenuate the proliferation of VSMCs activated by AngⅡ, which indicates that EGFR plays an important role in the proliferation of VSMCs activated by AngⅡ.
参考文献/References
［1］ Eguchi S,Numaguchi K, Iwasaki H, et al. Calciumdependent epidermal growth factor receptor transactivation mediates the angiotensin IIinduced mitogenactivated protein kinase activation in vascular smooth muscle cells ［J］. J Biol Chem, 1998,273(15):8890-8896.
［2］ Eguchi S,Dempsey PJ,Frank GD,et al. Activation of MAPKs by angiotensin II in vascular smooth muscle cells. Metalloproteasedependent EGF receptor activation is required for activation of ERK and p38 MAPK but not for JNK［J］. J Bio Chem, 2001,276(11):7957-7962 .
［3］ Kagiyama S, Eguchi S, Frank GD, et al. Angiotensioninduced cardiac hypertrophy and hypertensin are attenuated by epidermal growth factor recepor antisense ［J］. Circulation, 2002, 106(8):909-912 .
［4］ kagiyama S, Qian K, Kagiyama T, et al. Antisense to epidermal growth factor receptor prevents the Develoment of left ventricular hypertrophy ［J］ . Hypertension, 2003,41 ［3pt2］:824-829.
［5］ SchmidtOtt KM, Kagiyama S,Phillips MI. The multiple actions of angiotensin in atherosclerosis ［J］. Regul Pept, 2000, 93(1-3):65-77.
［6］ Asakura M, Kitakaze M, Takashima S, et al. Cardiac hypertrophy is inhibited by antagonism of ADAM12 processing of HBEGF: metalloproteinase inhibitor as a new therapy ［J］. Nat Med, 2002, 8(1);35-40.
［7］ Lucchesi PA, Sabri A, Belmadani S, et al. Involvement of metalloproteinases 2/9 in epidermal growth factor receptor transactivation in pressureinduced myogenic tone in mouse mesenteric resistance arteries ［J］. Circulation, 2004, 110(23):3587-3593.
［8］ Kagiyama S, Kagiyama T, Phillips MI, et al. Antisense oligonucleotides strategy in treatment of hypertension ［J］. Curr Opin Mol Ther, 2001, 3(3):258-264.
备注/Memo
- 备注/Memo:
- 收稿日期：2006-04-30.作者简介：朱艳霞,主治医师,硕士Email：zhuyx327@163.com
常用功能
统计/Statistics
- 摘要浏览/Viewed1531
- 全文下载/Downloads787
- 评论/Comments
