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rhCD40L及阿司匹林对人I型胶原α2（I）链启动子活性表达的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第5期
页码:
543-546
栏目:
临床研究
出版日期:
2007-10-01

文章信息/Info

Title:
Effect of Aspirin on humanα2(Ⅰ) collagen promoter induced by rhCD40L
作者:
樊民1; 李岚2; 王皓3; 高春芳3; 李洪涛1; 任雨笙1; 吴宗贵1
1.上海第二军医大学长征医院心内科，上海 200003;2.解放军411医院内4科; 3.长征医院实验诊断科
Author(s):
FAN Min;  LI Lan;  WANG Hao;  GAO Chunfang;  LI Hongtao;  REN Yushen;  WU Zonggui
Department of Cardiovasology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
关键词:
CD40配体; 阿司匹林; 胶原; 启动子
Keywords:
CD40 ligand;  aspirin; collagen; promoter
分类号:
R543;Q786
DOI:
-
文献标识码:
A
摘要:
目的 探讨rhCD40L及阿司匹林对人I型胶原α2（I）链启动子活性的作用。方法 体外培养人血管平滑肌细胞（VSMCs），人Ⅰ型胶原α2(Ⅰ)链基因启动子重组质粒pCOLH22.4和pCOLH21.6扩增和酶切鉴定后，通过脂质体（Lipofectamin）转染VSMCs，rhCD40L刺激转染后细胞，ELISA检测CAT目的基因表达以及阿司匹林（100 μmol/L）的影响。结果 rhCD40L使CAT目的基因表达下降；阿司匹林可以增加CAT目的基因表达，并逆转rhCD40L诱导的CAT目的基因表达下降（P<0.05）。结论 阿司匹林上调人I型胶原启动子活性表达，并且逆转rhCD40L对人I型胶原启动子活性的下调作用，可能是其抗动脉粥样硬化和稳定斑块的机制之一。
Abstract:
AIM To evaluate the effect of aspirin on humanα2(Ⅰ) collagen promoter induced by rhCD40L. METHODS After amplification and identification, pCOLH22.4 and pCOLH21.6, a plasmid containing PCAT3Enhancer, were transfected into human VSMCs using Lipofectamin transfection reagent. The expression of the target gene and protein were measured by CAT ELISA kit. The effects of aspirin on CAT activitywere evaluated after transfection. RESULTS After transfection, the activity of CAT reporter gene was assessed by ELISA method. Compared withthose in the control group, the CAT activities were inhibited by rhCD40L. Aspirin reversed the CAT activities inhibited by rhCD40L. CONCLUSION Aspirin upregulates the expression of humanα2(Ⅰ) collagen promoter and reverses promoter activities inhibited by rhCD40L. Thismay be one of the mechanism of aspirin in improving plaque stability.

参考文献/References

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［4］ Cyrus T, Sung S, Zhao L, et al. Effect of lowdose aspirin on vascular inflammation, plaque stability, and atherogenesis in lowdensity lipoprotein receptordeficient mice［J］. Circulation,2002,106(10):1282-1287.

［5］ Redondo S, SantosGallego CG, Ganado P, et al. Acetylsalicylic acid inhabits cell proliferation by involving transforming grouwth factorβ［J］. Circulation,2003,107(4):626-629.

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备注/Memo

备注/Memo:
收稿日期：2006-11-24.基金项目：国家重点基础研究发展规划项目资助（No.2005CB523309）通讯作者：吴宗贵，主任医师，主要从事冠心病临床和基础研究Email:zgwu@medmail.com.cn 作者简介：樊民，主治医师， 博士Email: fanmin@medmail.com.cn
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