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Title:: Effect of L-NAME microinjection into ventrolateral medulla on viscero-depressor response

作者:: 赵颖琦¹; 赵锁安²; 1.首都医科大学生理学教研室，北京 100069；2.白求恩军医学院生理学教研室，河北石家庄 050081

Author(s):: ZHAO Ying-qi¹; ZHAO Suo-an²; 1.Department of Physiology,Capital Medical University,Beijing 100069,China;2.Department of Physiology, Bethune Military Medical College, Shijiazhuang 050081, Hebei,China

关键词:: 内脏-减压反应; 内脏大神经传入纤维; N-硝基-L-精氨酸甲酯; 延髓腹外侧区头端; 延髓腹外侧区尾端; 一氧化氮

Keywords:: viscero-depressor response; greatersplanchnoafferents; L-NAME; rostral ventrolateral medulla; caudal ventrolateral medulla; nitric oxide

分类号:: R338.2

DOI:: -

文献标识码:: A

摘要:: 目的观察延髓腹外侧区头端（RVLM）和尾端（CVLM）微量注射N-硝基-L-精氨酸甲酯（L-NAME）对内脏-减压反应的影响，探讨静注L-NAME使该反应产生翻转的中枢机制。方法用电刺激内脏大神经传入纤维的方法模拟内脏痛，比较RVLM和CVLM微量注射L-NAME前后刺激神经时血压的变化情况。结果 RVLM给药后基础血压升高（P<0.01）；给药前刺激神经时为降压反应，△MBP=-(26.19±6.77)mmHg（1 mmHg=0.133 kPa），而给药后刺激时血压下降的效应被抵消，△MBP=-(15.56±6.63)mmHg（P<0.01）。CVLM给药后基础血压降低（P<0.01），给药前刺激神经时为降压反应，△MBP=-(28.78±11.71)mmHg，而给药后刺激时血压仍下降，但△MBP=-(16.63±9.30)mmHg（P<0.01）。若提前注入L-Arg，可以取消L-NAME的作用，即基础血压和刺激后的反应性血压的变化均恢复到原来的水平。结论 RVLM和CVLM微量注射L-NAME可部分抵消内脏－减压反应，提前注入L-精氨酸(L-Arg)可以取消L-NAME的上述作用。表明VLM参与了内脏减压反应并通过中枢NO通路起作用；静注L-NAME引起的翻转作用则是由于抑制了内源性NO的生成。

Abstract:: AIM To study the effect of microinjection of L-NAME into rostral ventrolateral medulla (RVLM) or caudal ventrolateral medulla (CVLM) on the viscerodepressor response and to probe into the central mechanism of the reversion of the response induced by intravenous injection of L-NAME. METHODS Visceral pain was imitated by electric stimulation of the greatersplanchnoafferents. The basal blood pressure (BP) and the pressure responses to the electric stimulation were observed before or after the microinjection of L-NAME into RVLM and CVLM. RESULTS The basal BP increased after the microinjection into RVLM (P<0.01). When the nerve was stimulated without the microinjection of LNAME into RVLM, BP decreased by -(26.19±6.77)mmHg, while the responsive BP decreased by -(15.56±6.63)mmHg following the microinjection of L-NAME (P<0.01). After the L-NAME microinjection into CVLM, the basal BP decreased (P<0.01). When the nerve was stimulated without the microinjection of L-NAME into CVLM, BP decreased by -(28.78±11.71)mmHg, while the response of BP was reduced partly to -(16.63±9.30)mmHg after microinjection of L-NAME (P<0.01). If L-argentine was microinjected before L-NAME microinjection, both the basal BP and the responsive BP were unchanged. CONCLUSION Microinjection of L-NAME into RVLM or CVLM can partly reduce the viscerodepressor response. VLM is involved in the viscerodepressor response and exerts its effect through the central NO pathway. Intravenous injection of L-NAME reverses the response possibly by inhibiting the intrinsic nitric oxide synthesis.

参考文献/References

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延髓腹外侧区微量注射L-NAME对内脏-减压反应的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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