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Title:: Effect of transplantation of autologous bone marrow mesenchymal stem cells on cardiac function after acute myocardial infarction

作者:: 金鹏; 金丽娟; 朱红; 宋湘; 李学奇; 哈尔滨医科大学附属第四医院心内科，黑龙江哈尔滨 150001

Author(s):: JIN Peng; JIN Lijuan; ZHU Hong; SONG Xiang; LI Xueqi; Department of Cardiology,Fourth Affiliated Hospital,Harbin Medical University,Harbin 150001,Heilongjiang,China

关键词:: 骨髓间充质干细胞; 细胞移植; 心肌梗死; 心功能

Keywords:: bone marrow mesenchymal stem cells; cell transplantation; acute myocardial infarction; cardiac function

分类号:: R541.1

DOI:: -

文献标识码:: A

摘要:: 目的观察骨髓间充质干细胞（MSCs）经冠脉移植对急性心肌梗死后心功能的影响。方法 24只日本大耳白兔，随机分为MSCs移植组(n=12)和培养液对照组(n=12)。从兔股骨抽取骨髓，体外培养MSCs。通过结扎左冠前降支建立急性心肌梗死模型。冠脉结扎后7 d，细胞移植组和对照组直接经冠脉注入MSCs和培养液。于心肌梗死前、细胞移植前、细胞移植后1、2和4 周对兔进行超声心动图检查。移植后4周处死动物，进行BrdU和第Ⅷ因子相关抗原免疫组化检测。结果移植后2周，MSCs移植组在射血分数（LVEF）和左室收缩末直径（LVESD）方面与移植前和对照组相比有显著改善（P<0.05）；移植4周后，LVEF、LVESD和左室舒张末直径（LVEDD）在MSCs移植组与移植前及对照组相比均有显著改善（P<0.05）。免疫组化检测发现，MSCs移植组BrdU染色阳性，血管计数较对照组明显增多（P<0.01）。结论经冠脉移植的MSCs可在梗死区心肌内存活并逐渐分化成心肌样细胞，促进毛细血管生成,显著改善心功能。

Abstract:: AIM To observe the effects of transplantation of autologous bone marrow mesenchymal stem cells（MSCs） via coronary artery on cardiac function after acute myocardial infarction. METHODS Twentyfour white rabbits were randomly divided into MSCs group (n=12)and IMDM culture medium control group (n=12). MSCs were isolated from the femoral bones and were cultured in vitro. The anterior descending branch of left coronary artery was ligated to establish animal models. MSCs were transplanted into myocardium via coronary artery 7 days after myocardial infarction in the cell transplantation group and the same volume of culture medium was transplanted into those in the control group. Cardiac function was evaluated before AMI and transplantation, and 1week, 2weeks and 4weeks after transplantation by echocardiography. The BrdU and factor VIII antigen were examined by immunohistochemical method 4weeks after transplantation. RESULTSTwo weeks after transplantation, left ventricular ejection fraction (LVEF) and left ventricular end systolic diameter (LVESD) improved significantly（P<0.05）compared with those before transplantation and in control group. Four weeks after transplantation, LVEF, LVESD and left ventricular end diastolic diameter (LVEDD) improved significantly（P<0.05）compared with those before transplantation and in control group. Immunohistochemical examination showed that BrdU was positive in MSCs group and the microvessel counts in MSCs group were much higher than those in control group（P<0.01）. CONCLUSION MSCs transplanted via coronary artery can survive in the infracted scar and differentiate into cardiomyocytes, promoting angiogenesis and improving cardiac function.

参考文献/References

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自体骨髓间充质干细胞移植对急性心肌梗死后心功能的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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