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不同年龄人骨髓间充质干细胞体外培养增殖的研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第1期
页码:
13-16
栏目:
基础研究
出版日期:
2008-01-20

文章信息/Info

Title:
Aging effect on growth and proliferation of human bone mesenchymal stem cells
作者:
王宇玫1; 张浩2; 李君2; 朱沈军2; 夏云峰1; 张津津1
1.解放军总医院第一附属医院干部病房， 北京100037；2. 北京市阜外心血管病医院再生医学研究室
Author(s):
WANG Yumei1;  ZHANG Hao2;  LI Jun2;  ZHU Shenjun2;  XIA Yunfeng1;  Zhang Jingjing1
1. 304 Clinical Division, PLA General Hospital, Beijing 100037, China; 2.Research Center for Cardiovascular Regenerative Medicine, Ministry of Health, FuWai Heart Hospital, CAMS and PUMC, Beijing 100037, China
关键词:
骨髓间充质干细胞;  年龄;  体外培养;  增殖性
Keywords:
bone mesenchymal stem cells;  aging;  in vitro culture;  proliferation
分类号:
R733.7；R542.2
DOI:
-
文献标识码:
A
摘要:
目的 本研究分析不同年龄段人骨髓间充质干细胞的生长和增生情况，探讨年龄对于骨髓间充质干细胞的影响。方法 采集61例不同年龄人的骨髓，分为少年组、中青年组和老年组，分离间充质干细胞，进行体外培养，观察其形态变化，对比分析其首次传代和每次传代时间、细胞克隆形成数量、细胞增长曲线的变化情况。结果 体外培养的干细胞在形态上无年龄差异，均成梭形纤维状，早期呈克隆状生长。细胞的首次传代时间从少年到老年呈逐渐增加的趋势，老年组明显长于另外两组；3组的体外培养倍增时间无明显差异，但老年组的干细胞克隆数量减少（差异尚未达到统计学意义），细胞的增殖显著低于另外两组（P<0.05）。结论 人骨髓间充质干细胞的生长增殖能力随着增龄而逐渐减退，尤其在老年组呈现出更明显的变化。
Abstract:
AIM To determine the effect of aging on the replicative capacity of bone mesenchymal stem cells (BMSC) in different age groups. METHODSBMSCs were isolated from 61 subjects of different ages and were divided in 3 groups: teenage, middle age and advanced age. Cells were cultured in medium and different measurements were made to observe their proliferation. RESULTS There were no visual differences in the shape and size in the first isolation between the 3 groups, but the old people experienced a little faster gradual loss of spindleformed features over cultivation and needed more time to reach the first confluence. The average time of reaching first confluence in teenage, middle age and advanced age group was (12.3±5.1)d, (13.6±5.8)d and (16.7±7.2)d, respectively. Double time during the following passages revealed no difference. Total CFUF numbers of BMSC decreased with aging and growth kinetic showed a slower increase in BSMCs from advanced age group compared with those in the other two groups. CONCLUSION Human BMSCs experience a gradual loss in its proliferation and replicate ability, which is more obvious in old people.

参考文献/References

［1］ Baksh D, Song L, Tuan RS. Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy［J］. J Cell Mol Med, 2004, 8(3): 301-316.

［2］ 武晓静,黄岚. 骨髓成体干细胞在心血管疾病发病中的病理生理意义［J］. 心脏杂志 , 2005,17(5): 479-482.

［3］ 江德勤,田杰,白永虹,等. 骨髓间充质干细胞移植在心肌病大鼠体内存活、分化的实验研究［J］. 心脏杂志 , 2005, 17(1) :4-7.

［4］ Sethe S, Scutt A, Stolzing A. Aging of mesenchymal stem cells［J］. Ageing Res Rev, 2006, 5(1): 91-116.

［5］ Fehrer C, Lepperdinger G. Mesenchymal stem cell aging［J］. Exp Gerontol, 2005, 40(12): 926-930.

［6］ Bonab MM, Alimoghaddam K, Talebian F, et al. Aging of mesenchymal stem cell in vitro［J］. BMC Cell Biol, 2006, 7(1): 14-21.

备注/Memo

备注/Memo:
收稿日期：2007-01-04.通讯作者：王宇玫，副主任医师，主要从事老年病、心血管病内科临床和基础研究Email:rose3042002@yahoo.com.cn
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