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姜黄素对内皮祖细胞功能及内皮型一氧化氮合酶的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第2期
页码:
136-141
栏目:
基础研究
出版日期:
2008-03-20

文章信息/Info

Title:
Effects of curcumin on endothelial progenitor cells function and endothelial nitric oxide synthase
作者:
吴钟伟; 张怀勤; 黄伟剑; 杨德业; 徐家窈
温州医学院附属第一医院心血管内科，浙江 温州 325000
Author(s):
WU Zhongwei;  ZHANG Huaiqin;  HUANG Weijian;  YANG Deye;  XU Jiayao
Department of Cardiology,First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325003, Zhejiang, China
关键词:
内皮祖细胞; 姜黄素; 内皮型一氧化氮合成酶
Keywords:
curcumin; endothelial progenitor cells; eNOS
分类号:
R543；R329.2
DOI:
-
文献标识码:
A
摘要:
目的 观察姜黄素对体外培养的人外周血内皮祖细胞（EPCs）数量及功能的影响。方法 采用贴壁选择法培养人外周EPCs，培养6 d后，收集贴壁细胞并加入姜黄素（0、5、10、15 μmol/L）培养一定时间（6、12、24和48 h）。荧光显微镜鉴定EPCs，分别观察EPCs的体外增殖、黏附、迁移、体外血管生成能力。Griess法检测NO分泌量，并RTPCR半定量测定eNOS基因表达。 结果 姜黄素增加外周血EPCs的数量，改善EPCs的体外增殖、黏附、迁移、体外血管生成能力（P<0.01）。并上调eNOS基因表达，促进EPCs来源的NO的释放（P<0.01）。结论 姜黄素增加体外EPCs数量及改善其功能，上调eNOS基因表达并增加EPCs来源的NO分泌。
Abstract:
AIM To investigate the effect of curcumin on endothelial progenitor cells (EPCs). METHODS Total mononuclear cells were isolated from human peripheral blood by ficoll density gradient centrifugation and were cultured. After 6 days of culture in vitro, EPCs were identified as adherent cells double positive for DiIacLDL/FITC2UEAⅠunder a laser scanning confocal microscope and their expression of CD34, CD31 and KDR was detected by flow cytometry. EPCs were then stimulated with curcumin (5, 10, 15 μmol/L) or vehicle control for 48 hours. The proliferation, migration, adhesion activity, vasculogenesis capacity of EPCS and the secretion of NO were assayed. RTPCR was also performed to determine the eNOS expression after curcumin interference. RESULTS Incubation of human EPCs with curcumin increased the proliferation, adhesion, migration activity and in vitro vasculogenesis capacity (P<0.01). Curcumin promoted the secretion of NO（ P<0.01）and upregulated the eNOS expression. CONCLUSION Curcumin promotes the secretion of NO and upregulates the eNOS expression of EPCs.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2007-08-20.基金项目：浙江省自然科学基金资助（303648） 通讯作者：张怀勤,主任医师，主要从事内皮祖细胞研究 Email：huaiqingzhang@126.com 作者简介：吴钟伟，硕士生Email:zhongwei_wu@yahoo.com.cn
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