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氧自由基对脂肪细胞中脂联素表达的下调作用 (PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第2期
页码:
142-146
栏目:
基础研究
出版日期:
2008-03-20

文章信息/Info

Title:
Expression of adiponeckine is downregulated by reactive oxygen species in adipocytes
作者:
陈宝莹1; 魏经国1; 王玮1; Xu Amin2; 于军3
第四军医大学:1. 唐都医院放射科，陕西 西安 710038,3.教学实验中心，陕西 西安 710032;2.香港大学内科系，香港
Author(s):
CHEN Baoying1;  WEI Jingguo1;  WANG Wei1;  XU Amin2;  YU Jun3
1.Department of Radiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi, China; 2.Department of Medicine, University of Hong Kong, Hong Kong; 3.Experiment Teaching Center, Fourth Military Medical University, Xi'an 710032, Shaanxi, China
关键词:
低氧; 氧自由基; 脂联素; 脂肪细胞
Keywords:
hypoxia;  reactive oxygen species ;  adiponectin;  adipocyte
分类号:
R329.25
DOI:
-
文献标识码:
A
摘要:
目的 研究脂肪细胞中氧自由基（reactive oxygen species，ROS）对脂联素（adiponectin，AD）表达的调节，并探讨其机制。方法 培养3T3L1细胞，并诱导其分化为脂肪细胞，以MTT比色法检测细胞的活性。采用HydroethidineTM（HE）及H2DCFDA测定细胞内ROS的产生。分别以定量PCR、多重免疫分析及夹心ELISA法，检测AD mRNA和其蛋白的表达水平。结果 低氧条件诱导脂肪细胞中ROS产生。作为一种重要的ROS，H2O2可剂量依赖性地抑制3T3L1脂肪细胞产生AD，并降低AD基因的转录激活因子增强子结合蛋白α（CCAAT/enhancer binding protein alpha，C/EBPα）及过氧化物酶体增殖激活受体γ（peroxisome proliferatoractivated receptor gamma，PPARγ）的表达。结论 低氧产生ROS，而ROS导致脂肪细胞中AD的表达抑制，其重要机制之一是ROS可下调C/EBPα和PPARγ，从而减弱AD基因的转录激活。
Abstract:
AIM To investigate the regulatory effects of ROS (reactive oxygen species) on the production of AD (adiponectin) in adipocytes and try to determine the underlying mechanisms. METHODS 3T3L1 cells were cultured and differentiated into mature adipocytes, and cell viability was measured by MTT. Intracellular ROS production was detected by HydroethidineTM（HE）and H2DCFDA. AD gene expression level was evaluated by quantitative realtime PCR. Quantification of the AD protein level secreted into conditioned medium was performed by multiplex immunoassay and sandwich ELISA. RESULTS In 3T3L1 adipocytes,the production of intracellular ROS was enhanecd by hyposia. As an important ROS, H2O2 dosedependently decreased AD production in 3T3L1 adipocytes. H2O2 exerted distinct inhibitory effects on expression of CCAAT/enhancer binding protein alpha (C/EBPα) and peroxisome proliferatoractivated receptor gamma (PPARγ), which are two key transcriptional factors regulating the expression of adipogenic genes. CONCLUSION Decrease of AD expression in adipocytes by ROS involves the decrease of C/EBPα and PPARγ.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2007-05-28.通讯作者：于军，讲师，博士，主要从事心血管疾病的研究 Email: yujunby@fmmu.edu.cn 作者简介：陈宝莹，主治医师，博士Email:chenby128@yahoo.com
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