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α-亚麻酸对高糖导致的人脐静脉内皮细胞损伤的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第3期
页码:
301-304
栏目:
基础研究
出版日期:
2008-05-20

文章信息/Info

Title:
Effects of alphalinolenic acid on damage of human umbilical vein endothelial cells induced by high concentration glucose
作者:
张薇1; 贾国良1; 王四旺2; 韩淑芳1; 布伦1
第四军医大学： 1.西京医院心血管内科，陕西 西安 710032,2.药物研究所，陕西 西安 710032
Author(s):
ZHANG Wei1;  JIA Guoliang1;  WANG Siwang2;  HAN Shufang1;  BU Lun1
1.Department of Cardiology, Xijing Hospital, 2.Institute of Materia Medica, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
α-亚麻酸; 糖尿病; 人脐静脉内皮细胞; 凋亡
Keywords:
alpha-linolenic acid(ALA);  diabetes mellitus;  human umbilical vein endothelial(HUVECs);  apoptosis
分类号:
R543.6;R587.1
DOI:
-
文献标识码:
A
摘要:
目的 观察α-亚麻酸(ALA)对高糖导致的人脐静脉内皮细胞(HUVECs)损伤的影响，探讨ALA在糖尿病血管并发症防治中的作用。方法 采用体外培养的第2～4 代HUVECs ,分为6 组,即正常对照组、高糖组(HG组)及HG＋ALA(ALA浓度分别为10、50、100和200 μmol/L)组。各组细胞于不同培养环境中培养72 h后收集标本，用MTT 比色法测定细胞活力，通过测定细胞上清液中一氧化氮(NO)和丙二醛(MDA)含量评估内皮功能，末端脱氧核糖核酸酶介导的dUTP 末端标记法( TUNEL 法) 和流式细胞仪法检测细胞凋亡情况。结果 较低浓度(10、50、100 μmol/L)的ALA组中，细胞存活率和合成NO浓度均显著高于高糖组(P<005)，MDA浓度和细胞凋亡率显著低于高糖组(P<005)；尤以50 μmol/L时为著。当ALA浓度增至200 μmol/L时，细胞存活率和合成NO浓度反而低于高糖组，MDA浓度和细胞凋亡率也较高糖组进一步增加。结论 ALA对高糖环境下培养的内皮细胞有着双重效应，既有保护作用也有细胞毒效应，与其浓度有关。
Abstract:
AIM To investigate the effects of alphalinolenic acid (ALA) on the proliferation and function of human umbilical vein endothelial cells (HUVECs) in presence of high concentration glucose. METHODS Cultured HUVEC were divided into six groups: control group, high glucose group (HG), groups of high glucose plus various concentrations of ALA (10, 50, 100 and 200 μmol/L). Cell samples were collected 72 hours later and cell proliferation was measured by MTT chromatometry. The endothelial function was evaluated by the concentration of NO and MDA in culture medium and apoptosis was determined by pyridine iodide staining on flow cytometry and tagged deoxynucleotide transferasemediated dUTP nick end labeling (TUNEL). RESULTS Pretreatment with low concentrations of ALA (10, 50 and 100 μmol/L) significantly improved the cell viability and NO production and decreased MDA production and the number of apoptotic cells, with a maximal effect at 50 μmol/L. But increasing ALA to 200 μmol/L produced the opposite effect. CONCLUSION Pretreatment with ALA induces a biphasic response in cell growth and function of HUVECs cultured in high glucose protective and cytotoxinic effect, which is related to the concentration of ALA.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2007-03-05.作者简介：张薇， 博士Email:zhangwei@fmmu.edu.cn
更新日期/Last Update: