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体外氧化修饰高密度脂蛋白对人单核细胞源性泡沫细胞内胆固醇流出的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第4期
页码:
373-375，380
栏目:
基础研究
出版日期:
2008-08-20

文章信息/Info

Title:
Effects of in vitro oxidized HDL on cholesterol efflux of human monocytederived foam cells
作者:
张秀丽1; 刘映峰1; 吴鹏1; 陈允钦2; 梁东辉2; 缪绯1
南方医科大学附属珠江医院： 1.心血管内科，2.中医科，广东 广州 510280
Author(s):
ZHANG Xiuli1;  LIU Yingfeng1;  WU Peng1;  CHEN Yuqin2;  LIANG Donghui2;  MIAO Fe1
1.Department of Cardiology, 2.Department of Traditional Chinese Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, Guangdong, China
关键词:
高密度脂蛋白; 氧化修饰; 泡沫细胞; 胆固醇; 流出
Keywords:
High density lipoprotein; Oxidative modification; Foam cells; Cholesterol; Efflux
分类号:
R329
DOI:
-
文献标识码:
A
摘要:
目的 探讨体外氧化修饰后的高密度脂蛋白(oxHDL)对人外周血单核细胞源性泡沫细胞内胆固醇流出的影响。方法 采用一次性密度梯度超速离心法从血浆中分离低密度脂蛋白(LDL)及高密度脂蛋白(HDL)，分别以Cu2+诱导法将其氧化修饰成oxLDL及oxHDL。采用密度梯度离心法和塑料吸附法从人外周血中分离出单核细胞，以50 nmol/L佛波酯（PMA）刺激48 h使之转化为巨噬细胞。细胞分为对照组（oxLDL组）、阳性对照组（HDL组）及实验组（oxHDL组），对照组仅加入终浓度为80 mg/L的oxLDL；HDL组和oxHDL组分别先加入终浓度均为50 mg/L的HDL和oxHDL共孵育1 h后，再分别加入终浓度为80 mg/L的oxLDL，并于实验的0、6、12及24 h测定细胞内总胆固醇（TC）、游离胆固醇（FC）及蛋白（Pro）含量。观察不同时间点各组细胞内TC/Pro比值的变化,并比较oxHDL与HDL对细胞内TC/Pro比值影响的时效关系。结果 成功地由体外氧化修饰了LDL及HDL并制备出理想的泡沫细胞模型。在实验的6、12及24 h，oxHDL组细胞内TC/Pro比值均较HDL组细胞高，差异具有统计学意义（P<005）。结论 体外氧化修饰后的HDL导致其胆固醇逆向转运（RCT）功能与效率的降低。
Abstract:
AIM To study the effects of oxidized high density lipoprotein (oxHDL) on cholesterol efflux of human monocytederived foam cells. METHODS Low density lipoprotein (LDL) and high density lipoprotein (HDL) were isolated from human plasma by one time density gradient centrifugation, and Cu2+ at concentration of 10 μmol/L was used to induce the oxidation of LDL (oxLDL). Monocytes were isolated from human peripheral blood by FicollHypaque density gradient centrifugation and plastic adsorptive process. The isolated cells were induced and stimulated by phorbol 12myristate 13acetate (PMA) at concentration of 50 nmol/L for 48 h to transfer them to macrophages. The cells were then allocated to control group (oxLDL group), positive control group (HDL group) and experimental group (oxHDL group). oxLDL alone at a final concentration of 80mg/L was added to control group, while HDL and oxHDL at 50 mg/L were coincubated in HDL group and oxHDL group for 1h, followed by addition of oxLDL at a final concentration of 80mg/L to both groups. The total cholesterol (TC), free cholesterol (FC) and protein (Pro) in cultured cells were detected at 6, 12 and 24 h of culture. The ratio of TC to Pro in cells at different time points was observed and the timeeffect relationship of HDL and oxHDL to cells was compared. RESULTS The isolated LDL and HDL were successfully oxidative modified in vitro and a human peripheral blood monocytederived foam cell model was established. Ratio of TC to Pro at 6, 12 and 24 h in oxHDL group was significantly higher than that of HDL group (P<005). CONCLUSION The reverse cholesterol transport (RCT) capacity and efficiency of HDL in cells decrease greatly following oxidization in vitro.

参考文献/References

[1] Thomas MJ, Chen Q, Zabalawi M, et al. Is the oxidation of highdensity lipoprotein lipids different than the oxidation of low density lipoprotein lipids?[J]. Biochemistry, 2001, 40(6):1719-1724.

[2] Jiang Y, Liu B W. Study on LOOH, CD, TBARS and REM kinetic changes during oxidation of high density lipoproteins induced by Cu2+ in vitro[J]. Acta Biochim Biophys Sin, 1999, 31(3):325-327.

[3] Salmon S, Maziere C, Auclair M. Malondialdehyde modification and copper induced autooxidation of high density lipoprotein decrease cholesterol efflux from human cultured fibroblasts[J]. Biochem Biophys Acta, 1992, 1125(2):230-235.

[4] Zhao Z, de Beer MC, Cai L, et al. Lowdensity lipoprotein from apolipoprotein Edeficient mice induces macrophage lipid accumulation in CD36 and scavenger receptor class Adependent manner[J]. Arterioscler Thromb Vasc Biol, 2005, 25(1):168-173.

[5] Han J, Hajjar DP, Febbraio M, et al. Native and modified low density lipoproteins increase the functional expression of the macrophage class B scavenger receptor,CD36[J]. J Biol Chem, 1997, 272(34):21654-21659.

[6] Meerchaert J, Furie MB. Monocytes use either CD11b/CD18 or VLA4 to migrate across human endothelium in vitro[J]. J Immunol, 1994, 152(2):1915-1920.

[7] 傅强，刘秉文. 氧化修饰使HDL促动脉平滑肌细胞胆固醇流出减少[J]. 生物化学与生物物理学报, 2000, 32（3）:248-252.

[8] Rifici VA, Khachadurian AK. Oxidation of high density lipoproteins: Characterization and effects on cholesterol efflux from J774 macrophages[J]. Biochem Biophys Acta, 1996, 1299(1):87-94.

[9] Ferretti G, Bacchetti T, Menanno F, et al. Effect of genistein against copperinduced lipid peroxidation of human high density lipoproteins(HDL)[J]. Atherosclerosis, 2004, 172(1):55261.

备注/Memo

备注/Memo:
收稿日期：2007-10-19.基金项目：广东省自然科学基金资助（06024444） 通讯作者：刘映峰，主任医师，主要从事冠心病的研究Email：yixiuzhang@126.com 作者简介：张秀丽，硕士Email:yixiuzhang@126.com
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