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OX-LDL及17β-雌二醇对体外人脐静脉内皮细胞ACE2 mRNA表达的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第4期
页码:
385-388，393
栏目:
基础研究
出版日期:
2008-08-20

文章信息/Info

Title:
Effect of oxidized low density lipoproteins and 17β-estrogen on mRNA expression of angiotensinconverting enzyme 2 in human vascular endothelial cells in vitro
作者:
陈文忠1; 李志樑1; 徐春生2; 周鹏1; 宋旭东1; 傅强1; 肖华1
南方医科大学附属珠江医院: 1.心内科, 2.急诊科, 广东 广州 510280
Author(s):
CHEN Wenzhong1;  LI Zhiliang1;  XU Chunsheng2;  ZHOU Peng1;  SONG Xudong1;  FU Qiang1;  XIAO Hua1
1.Department of Cardiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, Guangdong, China
关键词:
17β-雌二醇; 氧化型低密度脂蛋白; 血管紧张素转化酶2; 人脐静脉内皮细胞
Keywords:
17β-estrogen;  oxidized low density lipoprotein;  angiotensinconverting enzyme2;  human umbilical vein endothelial cells
分类号:
R363
DOI:
-
文献标识码:
A
摘要:
目的 探讨氧化低密度脂蛋白(ox-LDL)及17β-雌二醇(E2)对体外培养的人脐静脉内皮细胞(HUVECs)血管紧张素转化酶II（ACE2）表达的影响。方法 以不同浓度的ox-LDL(20,40,80 mg/L) 及 E2(2,10,50 nmol/L)分别单独刺激HUVECs 24 h并以40 mg/L的oxLDL与不同浓度的E2(2,10,50 nmol/L)共同刺激HUVECs 24 h。逆转录聚合酶链反应(RTPCR)检测ACE2 mRNA的表达情况。结果 与对照组比较,oxLDL单独刺激组ACE2 mRNA表达分别降低为074±008，052±007和031±006(均P<001),E2单独刺激组ACE2 mRNA表达分别增加为154±019，243±028和289±026(均P<001);40 mg/L的oxLDL与2，10，50 nmol/L E2共同刺激组, ACE2 mRNA表达分别为40 mg/L ox-LDL单独刺激组的195±038，256±032和322±037(3组间均P<005)。结论 ox-LDL呈浓度依赖性下调ACE2 mRNA表达；E2呈浓度依赖性上调ACE2 mRNA表达,且E2呈正向量效关系抑制ox-LDL引起的ACE2表达下调。
Abstract:
AIM To investigate the effect of oxidized low density lipoprotein（ox-LDL）and 17β-estrogen (E2) on mRNA expression of angiotensinconverting enzyme 2 (ACE2) in human umbilical vein endothelial cells (HUVECs). METHODS HUVECs were treated respectively with various concentrations of oxLDL (20,40,80 mg/L) and E2 (2,10,50 nmol/L) for 24 hours. In a combined experiment, HUVECs were treated with oxLDL(40 mg/L) and various concentrations of E2 (2,10,50 nmol/L) for 24 hours. Changes in mRNA expression of ACE2 were determined with reverse transcription polymerase chain reaction (RT-PCR). RESULTS ox-LDL induced a concentration dependent downregulation in mRNA expression of ACE2 and E2 induced a concentration dependent upregulation in mRNA expression of ACE2. Compared with that in control group, treatment of HUVECs with ox-LDL at the concentration of 20,40,80 mg/L stimulated 0.74±0.08, 0.52±0.07 and 0.31±0.06 fold downregulation in the ACE2 mRNA expression. Treatment of HUVECs with E2 at the concentration of 2, 10, 50 nmol/L stimulated 1.54±0.19, 2.43±0.28 and 2.89±0.26 fold upregulation in the ACE2 mRNA expression. Compared with treatment with oxLDL at the concentration of 40 mg/L group, the ACE2 mRNA expression of the combined group stimulated 1.95±0.38, 2.56±0.32 and 3.22±0.37 fold upregulation. CONCLUSION ox-LDL downregulates the mRNA expression of ACE2 and E2 upregulates the mRNA expression of ACE2 in HUVECs in a concentration dependent manner. E2 inhibits the oxLDL induced downregulation of ACE2 mRNA expression in a doseeffect relationship.

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备注/Memo

备注/Memo:
收稿日期：2007-09-03.基金项目：广东省自然科学基金资助（06301193） 通讯作者：李志樑，教授，主要从事冠心病的免疫机制研究 Email：Liz06@163.com 作者简介：陈文忠，硕士生Email：wenzhongchen1981@yahoo.com
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