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电磁脉冲辐照对大鼠心肌闰盘形态及ZO-1、Cx43蛋白表达的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第4期
页码:
389-393
栏目:
基础研究
出版日期:
2008-08-20

文章信息/Info

Title:
Effects of EMP on morphology of rat’s myocardial intercalated discs and the expression of Zo-1 and Cx43 proteins
作者:
李晓娟1; 任东青1; 周咏春1; 黄晓峰2; 王春梅2; 王爽2; 曾桂英1
第四军医大学: 1.军事预防医学系放射医学教研室,2.基础部电镜中心，陕西 西安 710032
Author(s):
LI XiaoJuan1;  REN DongQing1;  ZHOU YongChun1;  HUANG XiaoFeng2;  WANG ChunMei2;  WANG Shuang2;  ZENG GuiYing1
1.Department of Radiation Medicine, School of Preventive Medicine, 2.Center of Electron Microscope, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
电磁脉冲; 心肌; 闰盘; 缝隙连接蛋白Zo-1; 缝隙连接蛋白43; 大鼠
Keywords:
electromagnetic pulse;  myocardial;  intercalated discs;  gap junctions Zo-1;  gap junctions Cx43;  rat
分类号:
R331.3
DOI:
-
文献标识码:
A
摘要:
目的 探讨电磁脉冲（EMP）导致心肌闰盘(ID)改变的规律及机制，为制定EMP的卫生标准以及其医学应用提供实验依据。方法 采用EMP模拟发生器，场强为200 kv/m、脉冲次数为200次、脉冲间隔2s。雌性SD大鼠54只随机分为7组，其中对照组和照后12 h组均为12只，其余组（照后即刻、6、24、36及48 h）均为6只。将大鼠于照后即刻、6、12、24、36和48 h，采用硝酸镧示踪法和透射电子显微镜观察心肌ID结构的改变。照后12 h组,用免疫荧光标记法和激光共聚焦显微镜观察大鼠心肌中Zo1和Cx40蛋白表达的变化。 结果 正常对照组大鼠的心肌肌膜完整，肌小节清楚，肌丝排列整齐，细胞连接结构完整，ID连接紧密未见镧颗粒沉积。照后即刻组和6 h组的心肌ID间隙增宽不明显，仅见其间隙有少量镧颗粒；照后12 h组的心肌ID间隙增宽最明显，ID间隙中充满着大量镧颗粒；照后24 h组较照后12 h组明显好转，照后48 h组已基本恢复正常。照后12 h组和对照组的心肌细胞连接处Zo1、Cx43均有表达，但辐照组两种蛋白的平均荧光强度值均有下降的趋势，且无移位表达。结论 EMP辐照可诱导心肌ID间隙时相性增宽及Zo1和Cx43蛋白的表达下降。
Abstract:
AIM To explore the mechanism of intercalated discs (ID) caused by electromagnetic pulse (EMP) radiation so as to provide experimental basis for establishing sanitary standard and its medial application. METHODS EMP (200 kv/m field intensity, 200 pulse and 2s pulse interval from EMP simulator) was used in 54 female SD rats. The rats were randomly divided into seven groups, 12 rats in control group and 12 h group, and 6 rats in the other 5 groups (immediately, 6, 24, 36 and 48 h group). At different time points after EMP radiation (immediately, 6, 12, 24, 36 and 48 h), lanthanum nitrate tracer method and the transmission electron microscope (TEM) were used to observe the structural changes of left ventricular myocardium of the rats. At 12 h after EMP radiation, the changes of gap junctions Zo1 and gap junctions Cx43 were observed by fluorescent labeling and laserconfocal microscopy. RESULTS No obvious changes were observed under TEM in cardiac muscles of the rats after EMP radiation. The change in ID of cardiac muscle was most obvious at 12 h after radiation and a large amount of lanthanum nitrate. The change in ID of cardiac muscle was less obvious at 36 h than that at 12 h and only a small amount of lanthanum nitrate was seem. At 48 h after radiation, it became almost normal and no lanthanum nitrate was seen. Gap junctions Zo1 and gap junctions Cx43 expressed at the cardiac muscle cell conjunction in control and 12 h group, but expression tended to decrease in radiation groups. CONCLUSION After EMP exposure, the broadening of space between intercalated discs changes along with time. The changes in ID of cardiac muscle have reversibility by themselves. The expressions of gap junctions Zo1 and gap junctions Cx43 have a downtrend.

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备注/Memo

备注/Memo:
收稿日期：2007-08-03.基金项目：国家自然科学基金项目资助（30570447） 通讯作者：任东青，教授，主要从事放射医学研究Email:yrendq@fmmu.edu.cn 作者简介：李晓娟，硕士Email：lxj2008mail@126.com现工作单位：北京军区天津疗养院
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