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Title:: Effect of chronic multistress on ECG and HPA axis activity in rats

作者:: 李福平¹; 高凌云²; 肖颖彬¹; 第三军医大学新桥医院: 1.心血管外科，2.心血管内科，重庆 400037

Author(s):: LI Fuping¹; GAO Lingyun²; XIAO Yingbin¹; 1.Department of Cardiovascular Surgery, 2.Department of Cardiovascular Medicine, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China

关键词:: 慢性多重应激; 心电图; 下丘脑-垂体-肾上腺轴

Keywords:: Chronic multistress; ECG; HPA

分类号:: R540.41

DOI:: -

文献标识码:: A

摘要:: 目的研究慢性多重应激大鼠脱离应激源后心电图（Electrocardiogram,ECG）变化和下丘脑－垂体－肾上腺（hypothalamicpituitaryadrenocortical,HPA）轴应激反应。方法健康雄性SD大鼠20只随机分为应激组和对照组。选用刺激脉冲随机变动的噪声、足底电击、强迫游泳、束缚和夜间光照的复合刺激为应激组大鼠应激源，应激刺激持续30 d，建立大鼠慢性多重应激模型。于应激刺激前和完全脱离应激源后3 d记录心电图，分析血清促肾上腺皮质激素（ACTH）和皮质酮（corticosterone,Cor）的变化。结果刺激4周后，脱离应激源3 d，在1 h心电图记录期中，大鼠心律失常类型和次数明显多于应激前和对照组：持续窦性心动过速或窦性不齐、房性期前收缩（应激前14±07 vs 应激后402±5，P<001）、室性期前收缩（应激前31±06 vs 应激后557±4，P<001）、阵发性室速（应激前0 vs 应激后238±6，P<001）。心率明显增快［（3713±12）次/min→(5087±10)次/min, P<001］、RR间期明显缩短［（1716±5）ms→(1115±05)ms, P<001］、ST段抬高或下移、T波高耸直立或低平,血清ACTH［（531±4）ng/L→(2347±16)ng/L,P<001］和Cor［（16±4）μg/L→(279±50)μg/L,P<001］水平明显增高。结论慢性多重应激大鼠在脱离应激源后仍存在心律失常，并且HPA轴应激反应增高。

Abstract:: AIM To study the effect of chronic multistress on ECG and HPA axis activity in rats after exposure to multiple stressors. METHODS In this study a chronic multistress animal model was established in rats and the animals were subjected to 4 weeks of chronic multistress. Electrocardiographic recordings were performed before (baseline, B) and after (poststress, PS) chronic multistress. HPA axis activity was examined by collecting blood samples, which were analyzed for plasma ACTH and corticosterone (Cor) concentrations. The effect of chronic multistress on electrocardiogram (ECG) and serum ACTH and Cor was analyzed. RESULTS After 4 weeks of chronic multistress, a much higher incidence of arrhythmias including sinus tachycardia, sinus arrhythmia, atrial premature beats (B:14±07 vs PS: 402±5, P<001), ventricular premature beats(B:31±06 vs PS: 557±4, P<001) and ventricular tachycardia (B: 0 vs PS: 238±6, P<001) was produced. The chronic multistress also induced a much stronger increase of heart rate \[(3713±12)/min→(5087±10)/min, P<001\] and a reduction of average RR interval \[(1716±5) ms→(1115±05) ms, P<001\]. The ST of ECG was lightly displaced, T wave of ECG was upright lofty and serum ACTH \[(531±4)ng/L→(2347±16)ng/L, P<001\], Cor \[(16±4)μg/L→(279±50)μg/L, P<001\] level was raised compared with those in baseline and the control groups. CONCLSUSION Chronic multistress causes arrhythmias and increases HPA axis activity in rats following their exposure to multiple stressors.

参考文献/References

［1］ Sgoifo A, Buwalda B, Roos M, et al. Effects of sleep deprivation on cardiac autonomic and pituitaryadrenocortical stress reactivity in rats［J］. Psychoneuroendocrinology, 2006, 31(2):197-208.

［2］ Zipes DP, Rubart M. Neural modulation of cardiac arrhythmias and sudden cardiac death［J］. Heart Rhythm, 2006, 3(1):108-113.

［3］ Sears SF, Sowell LD, Kuhl EA, et al. The ICD shock and stress management program: a randomized trial of psychosocial treatment to optimize quality of life in ICD patients［J］. Pacing Clin Electrophysiol, 2007, 30(7):858-864.

［4］赵志刚，陈景藻，张李燕. 次声作用后大鼠血浆儿茶酚胺类物质含量的改变［J］. 心脏杂志， 2001， 13（4）：263-265,268.

［5］ Stone PH, Krantz DS, McMahon RP, et al. Relationship among mental stress induced ischemia and ischemia during daily life and during exercise: The Psychophysiologic Investigations of myocardial ischemia (PIMI) study［J］. J Am Coll Cardiol, 1999, 33(6):1476-1484.

［6］ Sgoifo A, de Boer SF, Westenbroek C, et al. Incidence of arrhythmias and heart rate variability in wildtype rats exposed to social stress［J］. Am J Physiol, 1997, 273(4):H1754-H1760.

［7］ Sgoifo A, Pozzato C, Costoli T, et al. Cardiac autonomic responses to intermittent social conflict in rats［J］. Physiol Behav, 2001, 73(3):343-349.

［8］ Costoli T, Bartolomucci A, Graiani G, et al. Effects of chronic psychosocial stress on cardiac autonomic responsiveness and myocardial structure in mice［J］. Am J Physiol Heart Circ Physiol, 2004, 286(6):H2133-H2140.

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慢性多重应激大鼠ECG变化和下丘脑－垂体－肾上腺轴应激反应增高(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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