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Title:: Effect of exogenous androgen at physiological level on expression of eNOS, iNOS in aortic wall of orchiectomized rats

作者:: 武强¹; 王晓武²; 郭豫涛³; 高萌³; 王雁⁴; 蔡军⁵; 解放军总医院南楼临床部： 1.医务部，3.老年心血管一科，北京100853； 2.广州军区总医院心外科，广东广州 510010； 4.济南军区烟台疗养院门诊部，山东烟台 264001； 5.海军钓鱼台干休所，北京 100072

Author(s):: WU Qiang¹; WANG Xiaowu²; GUO Yutao³; GAO Meng³; WANG Yan4; CAI Jun⁵; 1.Department of Medical Administration, 3.Department of Geriatric Cardiology, NanLou Clinical Department Division, General Hospital of PLA, Beijing 1008533, China； 2.Center of Cardiac Surgery, Guangzhou General Hospital, Guangzhou Military Area Command, G

关键词:: 雄激素; 内皮型NO合酶; 诱导型NO合酶; 去势大鼠

Keywords:: androgen; eNOS; iNOS; orchiectomized rats

分类号:: R743

DOI:: -

文献标识码:: A

摘要:: 目的探讨补充生理水平的外源性雄激素对去势大鼠动脉壁内皮型NO合酶（eNOS）和诱导型NO合酶（iNOS）表达的影响。方法将大鼠随机分为去势组、外源性生理水平雄激素补充组和对照组。采用实时荧光定量PCR和Western blot，观察不同处理组胸主动脉壁一氧化氮合酶(NOS)亚型eNOS和iNOS mRNA和其蛋白表达的变化。结果与对照组相比，去势组大鼠eNOS的表达显著降低（P<0.05），而外源性雄激素生理水平补充组大鼠eNOS的表达接近正常水平；两个处理组iNOS mRNA的水平与对照组比较均无统计学差异。结论补充外源性生理水平的雄激素对去势大鼠心血管系统的保护作用，可能是通过血管壁eNOS影响NO的合成与释放而介导的。

Abstract:: AIM To investigate the effects of exogenous androgen at physiological level on the expression of eNOS and iNOS in the aortic wall of orchiectomized rats. METHODS Animals were randomly divided into 3 groups: orchiectomized group, androgen treated at physiological level group and the sham control. Real-time RTPCR and Western Blotting were used to detect the expression of eNOS and iNOS at the mRNA and protein levels, respectively. RESULTS Compared with that in sham control group, the expression of eNOS in the orchiectomized group was significantly downregulated (P<005), but the expression of eNOS in the androgen treated group did not decrease. No significant difference in iNOS expression was found between the groups. CONCLUSION The protective effect of exogenous androgen at physiological level in orchiectomized rats is probably mediated by the synthesis and release of NO affected by eNOS of blood vessel endothelium.

参考文献/References

［1］武强,李小鹰,胥学伟. 外源性睾酮对去睾丸家兔性激素以及血脂和载脂蛋白水平的影响［J］. 中国应用生理学杂志， 2003， 19(3)：295-297.

［2］武强,李小鹰. 雄激素治疗男性冠心病及其机制［J］. 中华老年心脑血管病杂志, 2002, 3(3): 90-100.

［3］张中，郑强荪，马恒，等. 雄激素对离体大鼠心脏缺血/再灌注心功能及心肌凋亡的影响［J］. 心脏杂志, 2007， 19(3):272-276.

［4］ Canuelo A, Siles E, MartinezRomero R, et al. The nitric oxide system response to hypoxia/reoxygenation in the aged cerebral cortex［J］. Exp Gerontol, 2007, 42(12):1137-1145.

［5］聂莹雪，郭玫，禹红梅. 缺血再灌注损伤后nNOS、eNOS及iNOS表达的变化［J］. 中国血液流变学杂志, 2006, 16(3):332-340.

［6］ Yu J, Eto M, Akishita M, et al. Signaling pathway of nitric oxide production induced by ginsenoside Rb1 in human aortic endothelial cells: A possible involvement of androgen receptor［J］. Biochem Biophy Res Comm, 2007, 353(3):764-769.

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外源性生理水平的雄激素对去势大鼠动脉壁 eNOS和iNOS表达的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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