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Title:: Effect of testosterone on expression of eNOS, iNOS after focal cerebral ischemic reperfusion injury in rats

作者:: 王晓武; 张卫达; 王晓莉; 李杰; 袁彬彬; 广州军区广州总医院心脏外科中心，广东广州 510010

Author(s):: WANG Xiaowu; ZHANG Weida; WANG Xiaoli; LI Jie; YUAN Binbin; Centre of Cardiac Surgery, Guangzhou General Hospital, Guangzhou Military Area Command, Guangzhou 510010, Guangdong, China

关键词:: 脑缺血/再灌注; 雄激素; 内皮型NO合酶; 诱生型NO合酶; 大鼠

Keywords:: cerebral ischemic reperfusion; testosterone; eNOS; iNOS; rat

分类号:: R728

DOI:: -

文献标识码:: A

摘要:: 目的探讨雄激素对大鼠脑缺血/再灌注(I/R)损伤后，脑内内皮型一氧化氮合酶(eNOS)和诱生型一氧化氮合酶(iNOS)表达的影响。方法将大鼠45只随机均分为去势组、雄激素组和假手术组，喂养2周后，各组再按照I/R后不同时间点分为6,24和72 h3个亚组，共9组，每组均5只。采用Western blot 检测不同处理组脑内顶叶皮层中eNOS和iNOS表达的变化。结果随着再灌注时间的延长，各组大鼠eNOS的表达水平均有下降的趋势；而去势组和假手术组大鼠iNOS表达的水平均有上升的趋势。与假手术组相比，再灌注各时间点雄激素处理组eNOS的水平显著升高（P<0.01），去势组大鼠eNOS的水平明显降低（P<0.05）。而iNOS的表达水平则呈现出相反的趋势：即雄激素处理组明显低于正常水平（P<0.05）；而去势组大鼠则显著升高（P<0.05）。结论脑I/R后，在雄激素存在的情况下，eNOS的表达水平升高，而iNOS的表达水平降低；去势组eNOS的表达水平下降，而iNOS的表达水平上升，提示雄激素对脑I/R损伤可能具有保护作用。

Abstract:: AIM To explore the effects of different levels of testosterone on the expressions of eNOS and iNOS after focal cerebral ischemic reperfusion injury in rats. METHODS Fortyfive SD rats were randomly evenly divided into 3 groups: testosterone treated group, orchiectomized group and the sham operation group. The 3 groups were again devided into 3 subgroups (5 rats each group) according to different time points at 6 h, 24 h and 72 h after ischemic reperfusion. Western blot was used to detect the expressions of eNOS and iNOS in the parietal cortex of the rats. RESULTS The expression of eNOS was decreasing as the reperfusion time prolonged in each group, while the level of iNOS was upregulated in the orchiectomized and the sham control group. The eNOS level of the testosterone treated group was significantly higher than that of the control group (P<0.01), while it was much lower in the orchiectomized group (P<0.05) at each time points after reperfusion. But the expression pattern of iNOS was totally contrary: significant lower in the testosterone treated group (P<0.05) but much higher in the orchiectomized group (P<0.05). CONCLUSION The changing course of eNOS and iNOS after ischemic reperfusion injury is in positive and negative correlation with the level of testosterone, respectively. Testosterone probably has some protective effect on cerebral ischemic reperfusion injury.

参考文献/References

［1］ Blanco S, Castro L, Hernández R, et al. Age modulates the nitric oxide system response in the ischemic cerebellum［J］. Brain Res, 2007, 1157: 66-73.

［2］武强,李小鹰,胥学伟. 外源性睾酮对去睾丸家兔性激素以及血脂和载脂蛋白水平的影响［J］. 中国应用生理学杂志， 2003， 19(3)：295-297.

［3］武强,李小鹰. 雄激素治疗男性冠心病及其机制［J］. 中华老年心脑血管病杂志， 2002， 3(3)：90-100.

［4］聂莹雪，郭玫，禹红梅. 缺血再灌注损伤后nNOS、eNOS及iNOS表达的变化［J］. 中国血液流变学杂志， 2006， 16(3)：332-340.

［5］ Zea Longa EZ， Weinstein PR， Carlson S， et al. Reversible middle cerebral artery occlusion without craniectomy in rats［J］. Stroke， 1989， 20(1)：84-91.

［6］ Canuelo A, Siles E, MartinezRomero R, et al. The nitric oxide system response to hypoxia/reoxygenation in the aged cerebral cortex［J］. Exp Gerontol, 2007, 42（12）:1137-1145.

［7］ Yu J, Eto M, Akishita M, et al. Signaling pathway of nitric oxide production induced by ginseNoSide Rb1 in human aortic endothelial cells: A possible involvement of androgen receptor［J］. Biochem Biophysi Res Commun, 2007, 353（3）:764-769.

［8］Tsui JC, Baker DM, Shaw SG, et al. Alterations in nitric oxide synthase isoforms in acute lower limb ischemia and reperfusion［J］. Angiology, 2007, 58(5):586-592.

［9］ Arnlv J, Pencina MJ, Amin S, et al. Endogenous Sex hormones and cardiovascular disease incidence in men［J］. Ann Intern Med, 2006, 145(3)：176-185.

［10］张中，郑强荪，马恒，等. 雄激素对离体大鼠心脏缺血/再灌注心功能及心肌凋亡的影响［J］. 心脏杂志, 2007， 19(3)： 272-276.

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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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