我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

神经酰胺诱导内皮细胞凋亡机制的研究

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第1期
页码:
51-53
栏目:
基础研究
出版日期:
2009-02-28

文章信息/Info

Title:
Study on mechanism of endothelial cell apoptosis induced by ceramide
作者:
杨丽霞; 杨栋; 郭瑞威; 沈珠甫; 石燕昆; 齐峰; 王先梅
成都军区昆明总医院心血管内科，云南 昆明 650032
Author(s):
YANG Li-xia;  YANG Dong;  GUO Rui-wei;  SHEN Zhu-fu;  SHI Yan-kun;  QI Feng;  WANG Xian-mei
Department of Cardiology, Kunming General Hospital, Chengdu Military Area Command, Kunming 650032, Yunnan, China
关键词:
p53; Fas; 神经酰胺; 内皮细胞; 凋亡
Keywords:
p53;  Fas;  ceramide;  endothelial cell;  apoptosis
分类号:
Q545.3
DOI:
-
文献标识码:
A
摘要:
目的 探讨p53、Fas在外源性神经酰胺（C2-ceramide）诱导内皮细胞凋亡中的作用。方法 体外培养人脐静脉内皮细胞（HUVEC），以不同浓度的C2-ceramide处理后，用TUNEL染色法检测细胞的凋亡。用p53 的抑制剂PFT-α和Fas的抑制剂Fas相关磷酸酶-1（FAP-1）预处理细胞后,加入C2-ceramide，与对照组比较观察Fas的表达和细胞凋亡指数的变化。结果 C2-ceramide可剂量依赖性地诱导内皮细胞凋亡。FAP-1可抑制C2-ceramide诱导的内皮细胞凋亡；PFT-α不能抑制C2-ceramide诱导的内皮细胞凋亡。结论 神经酰胺能够诱导内皮细胞凋亡，其作用机制可能是通过Fas途径而非通过p53途径。
Abstract:
AIM To explore the role of p53 and Fas in human umbilical endothelial cells (HUVECs) apoptosis induced by ceramide. METHODS HUVECs were treated with different doses of C2-ceramide. The endothelial cells were cultured in vitro and treated with C2-ceramide alone or in combination with Fas-associated phosphalase-1 (FAP-1, an inhibitor of Fas) and PFT-α (an inhibitor of p53), respectively. The expression of Fas mRNA was measured by RT-PCR and the apoptosis of endothelial cells was detected by TUNEL staining. RESULTS C2-ceramide induces HUVEC apoptosis in a dose-dependent manner. FAP-1 (not PFT-α) inhibits the endothelial apoptosis by way of C2-ceramide. CONCLUSION It is via Fas, and not p53 that ceramide induces apoptosis of the endothelial cells.

参考文献/References

[1］ Colombaioni L, Garcia-Gil M. Sphingolipid metabolites in neural signalling and function［J］. Brain Res Brain Res Rev, 2004, 46(3):328-355.
［2］ Madan B, Singh I, Kumar A, et al. Xanthones as inhibitors of microsomal lipid peroxidation and TNF-alpha induced ICAM-1 expression on human umbilical vein endothelial cells (HUVECs) ［J］. Bioorg Med Chem, 2002, 10(11):3431-3436.
［3］ Sultan I, Senkal CE, Ponnusamy S, et al. Regulation of the sphingosine recycling pathway for ceramide generation by oxidative stress, and its role in controlling c-Myc/Max function［J］. Biochem J, 2006, 393(Pt2):513-521.
［4］ Gómez-Muoz A.Ceramide 1-phosphate/ceramide, a switch between life and death［J］. Biochim Biophys Acta, 2006, 1758(12):2049-2056.
［5］ Huang ST, Yang RC, Chen MY, et al. Phyllanthus urinaria induces the Fas receptor/ligand expression and ceramide-mediated apoptosis in HL-60 cells［J］. Life Sci, 2004, 75(3)：339-351.
［6］ Pascual M, Valles SL, Renau-Piqueras J, et al. Ceramide pathways modulate ethanol-induced cell death in astrocytes［J］. J Neurochem, 2003, 87(6):1535-1545.

备注/Memo

备注/Memo:
收稿日期：2008-2-28.基金项目：云南省自然科学基金项目资助（2006C0059M） 作者简介：杨丽霞，主任医师，博士 Email:doctorylixia@yahoo.com.cn
更新日期/Last Update: 2009-04-02