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DIDS对缺血/再灌注损伤大鼠心脏血流动力学、心律失常和心肌酶活性的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第2期
页码:
151-154/164
栏目:
基础研究
出版日期:
2009-03-30

文章信息/Info

Title:
Effects of DIDS on cardiac hemodynamic parameters, arrhythmia and CK/LDH activity of rats with ischemia/reperfusion injury
作者:
曹亚南1; 刘安恒1; 2; 张卫卫1; 师堂旺1; 刘艳1; 王晓明1
1.第四军医大学西京医院老年病科，陕西 西安710032；2.军事医学科学院附属307医院心内科，北京 100071
Author(s):
CAO Ya-nan1;  LIU An-heng1; 2;  ZHANG Wei-wei1;  SHI Tang-wang1;  LIU Yan1;  WANG Xiao-ming1
1.Department of Geriatrics, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, China; 2.Department of Cardiology, PLA 307 Hospital, Academy of Military Medical Sciences, Beijing, 100071, China
关键词:
DIDS; 缺血/再灌注损伤; 血流动力学; 心律失常; 心肌酶
Keywords:
DIDS;  ischemia/reperfusion injury;  hemodynamics;  arrhythmia;  myocardial enzyme
分类号:
R541
DOI:
-
文献标识码:
A
摘要:
目的 观察4,4’-二异硫氰基芪-2,2’-二磺酸（4,4’-diisothiocyanostilbene-2,2’-disulfonic acid，DIDS）对在体大鼠缺血/再灌注损伤（ischemia/reperfusion injure，I/RI）心脏的心律失常、心肌酶活性和血流动力学的影响。方法 雄性SD大鼠25只随机分为5组,每组5只：假手术组、I/R组、I/R+DIDS（7 mg/kg）组、I/R+DIDS（14 mg/kg）组及I/R+DIDS（28 mg/kg）组。于再灌注即刻，经股静脉以程控微量泵给予DIDS［4 ml/(kg·h)］ 2 h。于缺血前、后和再灌注后，记录心脏血流动力学指标、心电图变化，再灌注后测定各组血清肌酸激酶（CK）和乳酸脱氢酶（LDH）的活性。结果 DIDS可改善缺血 30 min/再灌注4 h，心脏的血流动力学指标，与假手术组相比，I/R组的左室舒张末期压（LVEDP）明显升高；而左室收缩压（LVSP）、左室内压上升的最大速率（＋dp/dtmax）和左室内压下降的最大速率（-dp/dtmax）明显下降（P<0.05）；降低心脏心律失常积分，再灌注期间，在假手术组、I/R组、I/R+DIDS （7 mg/kg）组、I/R+DIDS( 14 mg/kg)组、I/R+DIDS(28 mg/kg)组的心律失常积分值，分别是1.20±0.45，4.57±1.62，3.97±1.51，3.23±1.13和3.56±1.47，表明DIDS可明显抑制再灌注期心律失常（P<0.05），尤以I/R+DIDS(14 mg/kg)组为显著（P<0.01）；减少外周血CK和LDH的活性，与I/R组相比，DIDS组LDH和CK的活性明显降低（P<0.05）。结论 DIDS具有保护I/RI的作用，减少再灌注后的心律失常、抑制心肌酶释放和改善心脏的功能。
Abstract:
AIM To investigate the effect of 4,4’-Diisothiocyanostilbene-2,2’-disulfonic acid(DIDS) on the hemodynamic parameters, arrhythmia and creatine kinase(CK)/lacate dehydrogenase(LDH) of myocardium in the rat model of ischemia/reperfusion injure(I/RI) in vivo. METHODS SD rats (male) were divided into five groups; sham-operation group, I/R group, I/R+ DIDS (7 mg/kg) group, I/R+ DIDS (14 mg/kg) group and I/R+ DIDS (28 mg/kg) group, with 5 rats in each group. In DIDS group, the rats were administered by program controlled micro pump injection of DIDS 2 h ［4 ml/(kg·h)］ at the beginning of reperfusion. The hemodynamic parameters were determined by multiple channel electrophysiolograph before and after ischemia, and after reperfusion. The ECG changes were recorded incessantly from myocardial ischemia to reperfusion and the results were analyzed and given score. The activities of plasma CK and LDH were measured after reperfusion. RESULTS ①DIDS improved haemodynamics indexes. Compared with those in sham-operated group, left ventricular end diastolic pressure (LVEDP) significantly increased while left ventricular systolic pressure (LVSP), maximal rate of increase of ventricular pressure (dp/dtmax) and maximal rate of decrease of ventricular pressure (dp/dtmin) decreased markedly in I/R group (P<0.05). ②DIDS lowered the arrhythmia score. After 30-minute ischemia and 4-hour reperfusion, the arrhythmia score in sham-operation group, I/R group, DIDS (7 mg/kg) group, DIDS (14 mg/kg) group and DIDS (28 mg/kg) group were respectively 1.20 ±0.45, 4.57±1.62, 3.97±1.51, 3.23±1.13 and 3.56±1.47. DIDS markedly lowered the arrhythmia in reperfusion phase (P<0.05), especilly in 14 mg/kg group (P<0.01). ③DIDS significantly reduced the activities of plasma CK and LDH (P<0.05). CONCLUSION DIDS protects the heart by lowering arrhythmia in reperfusion phase, inhibiting release of LDH and CK and improving heart function during I/RI.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2008-6-20.基金项目：国家自然科学基金项目资助（30570758；30770847）；陕西省攻关课题项目资助［2005K13-G1(4)］ 通讯作者：王晓明，副教授，博士，主要从事心血管疾病的基础与临床研究Email:xmwang@fmmu.edu.cn 作者简介：曹亚南，硕士生Email:thiaa@sohu.com
更新日期/Last Update: 2009-04-16