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动脉粥样硬化与12/15-LOX基因启动子区甲基化状态的关系

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第2期
页码:
183
栏目:
基础研究
出版日期:
2009-03-30

文章信息/Info

Title:
The relationship between atherosclerosis and the status of 12/15-lipoxygenase gene promoter methylation
作者:
刘力1; 姜文瑞1; 刘雄涛1; 王洪涛1; 李军1; 俞翔2; 舒青3; 郑强荪1
第四军医大学:1.唐都医院心内科，陕西 西安 710038,3.基础部医学遗传学与发育 工程学教研室，陕西 西安 710032;2.解放军第五一八医院，陕西 西安 710043
Author(s):
LIU Li1;  JIANG Wen-rui1;  LIU Xiong-tao1;  WANG Hong-tao1;  LI-Jun1;  YU Xiang2;  SHU Qing3;  ZHENG Qiang-sun1
1.Department of Cardiology, Tangdu Hospital, Xi’an 710038, 3.Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, Xi’an 710032; 2.PLA 518 Hospital, Xi’an 710043, Shaanxi, China
关键词:
动脉粥样硬化;  12/15-脂氧合酶;  DNA甲基化
Keywords:
atherosclerosis;  12/15-lipoxygenase;  DNA methylation
分类号:
R543.5
DOI:
-
文献标识码:
A
摘要:
目的 探讨动脉粥样硬化（AS）与12/15-脂氧合酶（12/15-LOX）基因启动子区甲基化状态的关系。方法 将40只SD大鼠随机分为两组，即模型组和对照组，每组20只。模型组：给予高脂＋维生素D3粉剂饮食；对照组正常饮食。3个月后，处死大鼠，取其主动脉弓至腹主动脉的血管组织，用改进的半定量甲基化特异性PCR法检测血管组织中12/15-LOX基因启动子区甲基化状态。结果 ①经苏木精-伊红染色，显微镜下观察可见模型组大鼠的主动脉有明显的粥样斑块形成，对照组未见明显变化。②按照吸光度(A)比值把全部血管组织分为低甲基化状态和高甲基化状态，模型组大鼠的血管组织的低甲基化率显著高于对照组（P<0.01）。结论 血管斑块处12/15-LOX基因启动子区的甲基化状态与AS的形成相关，在AS发病过程中可能发挥着重要的作用。
Abstract:
AIM To explore whether atherosclerosis is related with the status of 15-lipoxygenase gene promoter methylation. METHODS 40 SD rats were randomly divided into two groups: experimental group and control group, 20 rats in each group. Feed the rats of experimental group by high-lipid and vitamin D3 overload for three months，while the rats of control group were normaly fed. Half-qualified methylation specific PCR was used to analyze the status of 15-lipoxygenase gene promoter of vascular tissue from aortic arch to abdominal aorta in the rats. And calculate the A value of the methylation specificity strap and the non-methylation specificity strap. RESULTS ①After HE staining, obvious atheromatous plaque has been formed in the experimental group, but there is no histopathology changes in the control group. ②The experimental group and control group were divided respectively into hypomethylated status and hypermethylated status. The incidence of experimental group in hypomethylated status（85％） is significantly higher than in the control group（25％）. CONCLUTION The status of 15-lipoxygenase gene promoter methylation of the vascular tissue is related with the formation of atherosclerosis, and may play an important role in the pathogenesis of atherosclerosis.

参考文献/References

[1］ Brash AR. Lipoxygenases: occurrence, functions, catalysis, and acquisition of Substrate［J］. J Biol Chem, 1999, 274(34):23679-23682.

［2］ Belkner J, Wiesner R, Rathman J, et al. Oxygenation of lipoproteins by mammalian lipoxygenases ［J］. Eur J Biochem, 1993, 213(1): 251-261.

［3］ 谭庆平,胡建安. 5-脂氧合酶与肿瘤的关系研究进展［J］. 毒理学杂志, 2007, 21(2):76-79.

［4］ 朱宏翔，胡建安. 脂氧合酶抑制剂的研究进展［J］. 中南大学学报(医学版), 2008, 33(6):541-547.

［5］ Yamamoto S, Katsukawa M, Nakano A, et al. Arachidonate 12-lipoxygenases with reference to their selective inhibitors［J］. Biochem Biophys Res Commun, 2005, 338(1):122-127.

［6］ Pratico D, Tangirala RK, Rader DJ, et al. Vitamin E suppresses isoprostane generation in vivo and reduces atherosclerosis in apoE-deficient mice［J］. Nat Med, 1998, 4(10):1189-1192.

［7］ Liu C, Xu D, Sjoberg J, et al. Transcriptional regulation of 15-lipoxygenase expression by promoter methylation［J］. Exp Cell Res, 2004, 297(1):61-67.

［8］ Herman JG, Graft JR, Myohanen S, et al. Methylation-specific PCR： a novel PCR assay for methylation status of CpG islands［J］. Proc Natl Acad Sci USA, 1996, 93(18):9821-9826.

备注/Memo

备注/Memo:
收稿日期：2008-9-27.通讯作者：郑强荪，主任医师，主要从事介入心脏病学研究Email:cartd@fmmu.edu.cn 作者简介：刘力，硕士生Email:easyxk@163.com
更新日期/Last Update: 2009-04-16