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|本期目录/Table of Contents|

骨髓间充质干细胞经静脉移植到心肌梗死大鼠体内后的分布

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第2期
页码:
201
栏目:
基础研究
出版日期:
2009-03-30

文章信息/Info

Title:
Distribution of bone marrow MSCs injected through subglossal vein into myocardial infarction model rats
作者:
何庚戌 1; 要彤2; 张浩3; 胡盛寿3; 张晓玲3; 魏英杰3
1.解放军第251医院胸心血管外科, 河北 张家口 075000；2.张家口北方学院第一附属医院心内科,河北 张家口 075000；3.中国协和医科大学、阜外心血管病医院、卫生部心血管疾病再生医学重点实验室,北京 100037
Author(s):
HE Geng-xu1;  YAO Tong2;  ZHANG Hao3;  HU Sheng-shou3;  ZHANG Xiao-ling3;  WEI Ying-jie3
1.Department of Thoracic and Cardiovascular Surgery, PLA 251 Hospital, Zhangjiakou 075000, Hebei, China; 2.Department of Cardiology, First Affiliated Hospital, North College of Zhangjiakou, Zhangjiakou 075000, Hebei, China; 3.Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences & Beijing Union Medical College, PUMC, Beijing 100037, China
关键词:
骨髓间充质干细胞; 心肌梗死; 细胞移植
Keywords:
bone marrow mesenchymal stem cells;  myocardial infarction;  cell transplantation
分类号:
R542.22
DOI:
-
文献标识码:
A
摘要:
目的 探讨经静脉注射骨髓间充质干细胞（MSCs）于心肌梗死大鼠模型后在体内的分布及对心功能的影响。方法 心肌梗死后1周将标记的MSCs注射到大鼠舌下静脉内，在细胞移植后不同时间点取心、肝、脾、肺、肾脏器，进行组织学检查观察移植细胞的分布。通过心脏超声复查移植后3周、6周心功能改变情况。结果 MSCs 经静脉注射后各脏器的组织结构无明显异常改变。移植细胞在早期主要分布在正常心肌组织内，1周后主要分布在梗死及交界区内，正常心肌组织内很少见细胞存留。细胞移植后3周、6周实验组左心室没有进一步扩大，心功能没有进一步恶化。结论 静脉注射移植MSCs后细胞可以分布到重要组织器官内，并不影响其组织结构；移植细胞有向梗死心肌组织内迁移的趋势，能明显延缓左心室重构而导致的心功能恶化。
Abstract:
AIM To study the distribution of MSCs injected intravenously and the feasibility of this approach used to repair the infracted heart. METHODS Bone marrow MSCs were injected into the subglossal vein of the SD rats 7 days after myocardial infarction was created and the organs such as the lungs, heart, liver, kidneys and spleen were harvested at different times after transplantation. In order to assess the efficiency of cell transplantation by this approach, another 24 SD rats were given MSCs or medium and after 3 weeks and 6 weeks, the diameters and function were evaluated through transthoracic echocardiography. RESULTS After injection through the subglossal vein, the MSCs passed through the capillary of the lung, and then distributed into the heart and non-targeted organ, such as the spleen, kidneys, lungs, but seldom in the liver. The structure of these organs remained normal. The cells migrating into myocardium had a tendency to the infracted area. The left ventricle function did not exacerbate after injection of MSCs compared with that in control group. CONCLUSION MSCs injected intravenously can distribute into myocardium, spleen, kidneys and lungs. The migration of injected cells delays the left ventricle remodeling after MI.

参考文献/References

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［2］ 王晓旭,于玲范,姜雪松，等. 骨髓干细胞对急性梗死心肌修复的实验研究［J］. 心脏杂志， 2005， 17（1）：31-33,36.

［3］ 徐瑾，王彬尧，王长谦，等. 骨髓间充质干细胞在心肌梗死区的移植和分化对心肌梗死后心力衰竭的影响［J］. 心脏杂志， 2006， 18（2）：138-142.

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［6］ Chen J, Li Y, Wang L, et al. Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats ［J］. Stroke, 2001, 32(4):1005-1011.

［7］ Chen J, Zhang ZG, Li Y, et al. Intravenous administration of human bone marrow stromal cells induces angiogenesis in the ischemic boundary zone after stroke in rats ［J］. Circ Res, 2003, 92(6):692-699.

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［9］ Strauer RE, Brehm M, Zeus T, et al. Repair of infracted myocardium by autologous intracoronary mononuclear bone marrow cell transplantation in humans［J］. Circulation, 2002, 106(15):1913-1918.

［10］Kraitchman DL, Tatsumi M, Gilson WD, et al. Dynamic imaging of allogeneic mesenchymal stem cells trafficking to myocardial infarction［J］. Circulation, 2005, 112(10):1451-1461.

［11］Kucia M, Dawn B, Hun G, et al. Cells expressing early cardiac markers reside in the bone marrow and are mobilized into the peripheral blood after myocardial infarction［J］. Circ Res, 2004, 95(12):1191-1199.

［12］Gao J, Dennis JE, Muzic RF, et al. The dynamic in vivo distribution of bone marrow-derived mesenchymal stem cells after infusion［J］. Cells Tissues Organs, 2001, 169(1):12-20.

［13］Barbash IM, Chouraqui P, Baron J, et al. Systemic delivery of bone marrow-derived mesenchymal stem cells to the infarcted myocardium： feasibility, cell migration, and body distribution ［J］. Circulation, 2003, 108(7):863-868.

备注/Memo

备注/Memo:
收稿日期：2007-7-24.通讯作者：胡盛寿，主任医师，主要从事终末期心脏病的治疗研究Email：huss@163bj.com 作者简介：何庚戌， 副主任医师，博士Email:hegengxu@yahoo.com.cn
更新日期/Last Update: 2009-04-16