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Staurosporine诱导心肌细胞氯通道电流的电生理学特性

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第3期
页码:
292-295
栏目:
基础研究
出版日期:
2009-05-15

文章信息/Info

Title:
Electrophysiological characteristics of chloride channel currents in cardiomyocytes induced by staurosporine
作者:
刘艳; 张卫卫; 李源; 王晓明
第四军医大学西京医院老年病科，陕西 西安 710032
Author(s):
LIU Yan;  ZHANG Wei-wei;  LI Yuan;  WANG Xiao-ming
Department of Geriatrics, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
心肌细胞; staurosporine; 容积敏感性氯通道电流; 全细胞记录
Keywords:
cardiomyocytes;  staurosporine;  volume-sensitive Cl-channel currents;  whole-cell patch clamp
分类号:
R331.38
DOI:
-
文献标识码:
A
摘要:
目的 探讨staurosporine（STS）诱导乳鼠心肌细胞凋亡的早期，凋亡性容积减少（AVD）发生时，是否有氯通道电流的产生及其电生理学特性。方法 分别采用低渗组的灌流液和含STS的等渗组灌流液处理原代培养的SD乳鼠心肌细胞，以膜片钳全细胞记录法记录电流。结果 ①以低渗灌流液处理的心肌细胞时，可记录到氯通道电流。该电流呈现类似容积敏感性氯通道电流(volume-sensitive chloride channel current,ICl,Vol)的电生理学特性：即外向整流性、高电位刺激下的时间依赖性失活及对氯通道阻断剂4,4′-异二硫氮氐2,2′-二磺酸(DIDS)的敏感性。②以含4 μmol/L STS的等渗液灌流心肌细胞时，也可记录到类似低渗诱导产生的氯通道电流，具有ICl,Vol的电生理学特性，且用氯通道阻断剂500 μmol/L DIDS后，在+40 mV、+60 mV、+80 mV及+100 mV时，能够明显电压依赖性地阻断该电流。结论 首次应用STS在培养乳鼠心肌细胞中记录到类似容积敏感性氯通道电流。
Abstract:
AIM To explore whether chloride channel currents are present when apoptotic volume decrease (AVD) happens during the early times of neonatal rat cardiomyocytes apoptosis induced by staurosporine (STS), and to study the electrophysiological properties of chloride channel currents. METHODS Primary cultured neonatal rat cardiomyocytes were subjected to hypotonic and isotonic perfusion containing 4 μmol/L STS, respectively. We used whole-cell patch-clamp techniques to record chloride channel currents.RESULTS ①After exposure of cardiac myocytes to hypotonic solution, we recorded chloride channel currents which resembled volume-sensitive chloride channel currents (ICl,Vol): outward rectification, time dependent inactivation, sensitivity to 4,4′-diisothiocya-natostilbene-2,2′-disulfonicacid (DIDS). ②After exposure of cardiac myocytes to 4 μmol/L STS isotonic solution, chloride channel currents showing characterizations of ICl,Vol were also recorded. The currents were voltage dependently blocked by 500μmol/L DIDS at +40mV,+60mV,+80mV,+100mV. CONCLUSION It’s the first time to record similar volume-sensitive outwardly rectifying Cl-currents by STS in primary cultured neonatal rat cardiomyocytes.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2008-10-21.基金项目：国家自然基金课题项目资助（30570758，30770847） 通讯作者：王晓明，副教授，博士，主要从事心血管疾病的基础与临床研究Email:xmwang@fmmu.edu.cn 作者简介：刘艳，硕士生Email:liuyanjelly@163.com
更新日期/Last Update: 2009-05-18