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|本期目录/Table of Contents|

大鼠甲状旁腺素对体外培养心室肌细胞的促肥大作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第3期
页码:
336-339
栏目:
基础研究
出版日期:
2009-05-15

文章信息/Info

Title:
Hypertrophic effect of rat parathyroid hormone in cultured ventricular cardiomyocytes in vitro
作者:
刘晓刚1; 任野平1; 张伟兵2; 隋满姝3; 郭艳1; 赵钰1; 刘哲1; 刘素雁1
哈尔滨医科大学:1.附属第二医院肾内科，黑龙江 哈尔滨 150086,2.附属第四医院 内科，黑龙江 哈尔滨 150036，3.附属第一医院肾内科，黑龙江 哈尔滨 150036
Author(s):
LIU Xiao-gang1;  REN Ye-ping1;  ZHANG Wei-bing2;  SUI Man-shu3;  GUO Yan1;  ZHAO Yu1;  LIU Zhe1;  LIU Su-yan1
1.Renal Division, Second Affiliated Hospital to Haerbin Medical University, Harbin 150086, Heilongjiang, China, 2.Medical Department, Fourth Affiliated Hospital to Haerbin Medical University，Harbin 150036, Heilongjiang, China, 3.Renal Division, First Affi
关键词:
甲状旁腺素1-34; 心肌细胞; 肥大; 细胞外信号调节激酶
Keywords:
parathyroid hormone1-34;  cardiac myocytes;  hypertrophy;  extracellular signal regulated kinases
分类号:
R582
DOI:
-
文献标识码:
A
摘要:
目的 观察不同浓度大鼠甲状旁腺素1-34（rat parathyroid hormone 1-34，rPTH1-34）对体外培养心室肌细胞的致肥大作用，观察细胞外信号调节激酶1/2（extracellular signal regulated kinases 1/2，ERK1/2）的变化。方法 体外培养新生大鼠心室肌细胞，以1×10-8～1×10-6 mol/L rPTH1-34分别孵育24 h，测定细胞直径、3H-亮氨酸(3H-Leu)掺入率、心房钠尿肽（atrial natriuretic peptide，ANP）mRNA、ERK1/2、磷酸化ERK1/2(p-ERK1/2)蛋白的表达。结果 与正常组相比，1×10-8 mol/L rPTH 1-34可使体外培养的心肌细胞直径、细胞蛋白合成速率轻度增加，ANP mRNA表达轻度升高，但无统计学意义；1×10-7，1×10-6 mol/L rPTH1-34 均可显著增加心肌细胞直径（P<0.01），细胞蛋白合成速率（P<0.01）和ANP mRNA表达（P<0.01）。与正常组比较，PTH呈浓度依赖性地增加了ERK1/2和p-ERK1/2的表达（P<0.01）。结论 1×10-7，1×10-6 mol/L rPTH1-34能在体外诱导新生大鼠心室肌细胞发生肥大反应，且呈一定的浓度依赖性；PTH能呈浓度依赖性地刺激心肌ERK1/2过度表达。
Abstract:
AIM To explore the hypertropic effect of different concentrations of rat parathyroid hormone1-34 (rPTH1-34) in ventricular cardiomyocytes in vitro and to investigate the changes of extracellular signal regulated kinases 1/2 (ERK1/2). METHODS Hypertrophy in neonatal rat cadiomyocytes was induced by 1×10-8～1×10-6 mol/L rPTH1-34 in 24 h in vitro. Cellular diameter, 3H-Leucine incorporation and mRNA expression of atrial natriuretic peptide (ANP) were detected, and protein expression of ERK1/2 and phospho-ERK1/2 (p-ERK1/2) was measured. RESULTS Compared with that in normal control group, 1×10-8 mol/L rPTH1-34 slightly increased single cellular diameter, the synthetic rate of protein and mRNA expression of ANP but with no statistical significance. Both 1×10-7 and 1×10-6 mol/L rPTH1-34 markedly increased single cellular diameter (P<0.01), the synthetic rate of protein (P<0.01) and mRNA expression of ANP (P<0.01). Compared with that in normal control group, PTH increased protein expression of ERK1/2 and p-ERK1/2 in a concentration-depended manner (P<0.01). CONCLUSION 1×10-7 and 1×10-6 mol/L rPTH 1-34 induce hypertrophy in neonatal rat cadiomyocytes in vitro in a concentration dependent manner. PTH leads to the over-expression of ERK1/2 in a concentration-dependent manner.

参考文献/References

[1］ Fujii H, Kim JI, Abe T, et al. Relationship between parathyroid hormone and cardiac abnormalities in chronic dialysis patients［J］. Intern Med, 2007, 46(18):1507-1512.

［2］ Mitsnefes MM, Kimball TR, Kartal J, et al. Progression of left ventricular hypertrophy in children with early chronic kidney disease: 2-year follow-up study［J］. J Pediatr, 2006, 149(5):671-675.

［3］ Yang L, Zou X, Liang Q, et al. Sodium tanshinone IIA sulfonate depresses angiotensin II-induced cardiomyocyte hypertrophy through MEK/ERK pathway［J］. Exp Mol Med, 2007, 39(1):65-73.

［4］ 朱肖星，梅其炳，刘莉，等. 内皮素-1对新生大鼠心肌细胞的影响及机制［J］. 心脏杂志， 2005, 17(5):441-444.

［5］ De Jonge HW, Dekkers DH, Houtsmuller AB, et al. Differential signaling and hypertrophic responses in cyclically stretched vs endothelin-1 stimulated neonatal rat cardiomyocytes［J］. Cell Biochem Biophys, 2007, 47(1):21-32.

［6］ Joy MS, Karagiannis PC, Peyerl FW. Outcomes of secondary hyperparathyroidism in chronic kidney disease and the direct costs of treatment［J］. J Manag Care Pharm, 2007, 13(5):397-411.

［7］ Tomilina NA, Storozhakov GI, Gendlin GE, et al. Risk factors and pathogenetic mechanisms of left ventricular hypertrophy in progressive chronic kidney disease and after transplantation of the kidney［J］. Ter Arkh, 2007, 79(6):34-40.

［8］ Krauser DG, Devereux RB. Ventricular hypertrophy and hypertension: prognostic elements and implications for management［J］. Herz, 2006, 31(4):305-316.

［9］ 杨丽霞，王先梅，石燕昆，等. 细胞外信号调节激酶的变化在心力衰竭患者心肌重构中的意义［J］. 心脏杂志， 2006， 18(1):62-64.

备注/Memo

备注/Memo:
收稿日期：2007-11-5.通讯作者：刘素雁，教授，主要从事尿毒症心肌病的发病机制及治疗的研究Email：hrb-lxg@163.com 作者简介：刘晓刚，主治医师，博士Email：hrb-lxg@163.com
更新日期/Last Update: 2009-05-18