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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2009年第3期

页码:

375-377

栏目:

临床研究

出版日期:

2009-05-15

文章信息/Info

Title:

Effect of ischemic postconditioning on myocardial ischemia/reperfusion injury in hyperlipemia rats

作者:

刘胜辉; 霍玉娥; 尹博英

河北大学附属医院心内科，河北 保定 071000

Author(s):

LIU Sheng-hui;  HUO Yu-e;  YIN Bo-ying

Department of Cardiology, Affiliated Hospital to Hebei University, Baoding 071000, Hebei, China

关键词:

高血脂; 再灌注损伤; 后处理

Keywords:

hyperlipemia;  reperfusion injury;  postcondioning

分类号:

R619.9

DOI:

-

文献标识码:

A

摘要:

目的 观察缺血后处理对高血脂大鼠缺血/再灌注（I/R）心肌的保护作用。方法 选择高血脂SD大鼠36只，随机分为3组，即假手术组、I/R组和缺血后处理组，每组12只。制备大鼠心肌I/R模型。I/R组：收紧结扎线缺血40 min,放松结扎线再灌注240 min。缺血后处理组:缺血40 min后,再灌注10 s,缺血10 s,连续3个循环，然后再灌注240 min。假手术组：开胸后穿线做套环，但不收紧结扎线。用全自动生化仪测定血清肌酸激酶（CK）的含量，黄嘌呤氧化酶法检测超氧化物岐化酶（SOD）的活性。采用硫代巴比妥酸法测定丙二醛（MDA）的含量，用伊文氏蓝-红四氮唑（TTC）染色法测定心肌梗死的范围。结果 ①血清CK活性的测定：再灌注结束后，缺血后处理组和I/R组的活性CK的活性明显高于假手术组［分别为（712.94±20.68）、（946.58±27.43） vs （232.12±18.26）U/L，P<0.05，1 U=16.67 nkat］,缺血后处理组明显低于I/R组(P<0.05)。②血清SOD和MDA的含量：缺血后处理组血清SOD 的含量高于对照组(P<0.05)；MDA的含量明显低于对照组(P<0.05)。③心肌梗死范围：再灌注结束后，缺血后处理组和I/R组的心肌缺血区与左室面积的比率无明显差异。缺血后处理组的心肌坏死区与缺血区的比率显著低于I/R组［分别为(25.3±6.6）% vs （39.2±7.1）%，P<0.05］。结论 缺血后处理对高血脂大鼠I/R心肌具有保护作用。

Abstract:

AIM To study the effect of ischemic postconditioning on myocardial ischemia/reperfusion injury in hyperlipemia rats. METHODS Thirty-six hyperlipemia SD rats were selected and randomly divided into 3 groups: sham-operation group, ischemic reperfusion group and ischemic postconditioning group, with 12 rats in each group, and MIR models were established. Rats in the ischemic reperfusion group were ligated for a 40-minute ischemia, and then were reperfused for 240 minutes. Rats in the ischemic postconditioning group were ligated for a 40-minute ischemia, reperfused for 10s and 10s ischemia for three cycles, and then reperfused for 240 minutes. In the sham-operation group, the chests of the rats were opened to braid for ringer without ligation. The activity of creatine kinase, SOD, MDA and myocardial infarct size were measured. RESULTS ①The serum creatine kinase activity was obviously higher in the ischemic postconditioning group and ischemic reperfusion group after reperfusion compared with that in the sham-operation group ［which were respectively（712.94±20.68）, （946.58±27.43） and （232.12±18.26）U/L, P<0.05, 1 U=16.67 nkat］. The serum creatine kinase activity was significantly lower in the ischemic postconditioning group than that in the ischemic reperfusion group (P<0.05). ②The activity of SOD was much higher in the ischemic postconditioning group than that in the ischemic reperfusion group (P<0.05). The activity of MDA was lower in the ischemic postconditioning group than that in the ischemic reperfusion group (P<0.05). ③No significant difference was found in the ratio of myocardial ischemic area and the size of left ventricle after reperfusion in the ischemic postconditioning group and ischemic reperfusion group. The ratio of myocardial necrosis area and the myocardial ischemic area was significantly lower in the ischemic postconditioning group compared with that in the ischemic reperfusion group (P<0.05). CONCLUSION Ischemic postconditioning relieves the ischemic reperfusion injury in hyperlipemia rats.

参考文献/References

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备注/Memo

备注/Memo:

收稿日期：2008-9-25.作者简介:刘胜辉,主治医师,博士Email:markliul@163.com

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更新日期/Last Update: 2009-05-18