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粒细胞集落刺激因子动员内皮祖细胞治疗大鼠急性心肌梗死的疗效观察(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第4期
页码:
467-470
栏目:
基础研究
出版日期:
2009-06-25

文章信息/Info

Title:
Therapeutic effects of G-CSF on rats with myocardial infarction by endothelial progenitor cell mobilization
作者:
陈婷婷1; 米卫东2; 王刚1; 李力兵1; 高长青1
解放军总医院：1.心血管外科，2.麻醉科，北京 100853
Author(s):
CHEN Ting-ting;  MI Wei-dong;  WANG Gang;  LI Li-bing;  GAO Chang-qing
Department of Cardiovascular Surgery, PLA General Hospital, Beijing 100853, China
关键词:
粒细胞集落刺激因子; 内皮祖细胞; 心功能; 大鼠
Keywords:
granulocyte-colony stimulating factor;  endothelial progenitor cells;  cardiac function
分类号:
Q592.1
DOI:
-
文献标识码:
A
摘要:
目的 通过对心脏在体心功能和离体心肌储备功能测定，评价粒细胞集落刺激因子(G-CSF)动员内皮祖细胞(EPCs)对心肌梗死(MI)大鼠心功能改善的作用。方法 雄性Wistar大鼠36只，随机分为对照组、MI组和G-CSF组(每组12只)，MI组和G-CSF组采用冠脉左前降支结扎法制作MI模型后，分别腹腔注射生理盐水和G-CSF连续5 d。给药后第7天，球后静脉采血，测定EPCs的数目和血浆血管内皮生长因子(VEGF)及C反应蛋白(CRP)的浓度。第4周时，进行在体心功能和离体心肌储备功能的测定，对MI大鼠的心功能进行全面评价。结果 G-CSF可增加外周血中EPCs的数目，提高血浆中VEGF的浓度。同时在体心功能和离体心肌储备功能测定的结果显示，MI大鼠的心功能得到改善。结论 G-CSF能够促进心梗大鼠EPCs的动员，增强VEGF的释放，改善MI大鼠的心功能。因此，G-CSF用于EPCs动员治疗AMI在临床上是有应用前景的。
Abstract:
AIM: To investigate the effects of endothelial progenitor cell (EPC) mobilization with granulocyte-colony stimulating factor (G-CSF) on neovascularization and to evaluate cardiac function. METHODS: Thirty six adult male rats were divided randomly into control group (saline), myocardial infarction group (MI) and G-CSF treated group ［G-CSF 30 g/(kg·d)］ with saline or G-CSF IP injected for 5 days. The number of EPCs was determined and serum concentrations of VEGF and CRP were measured on the seventh day. Cardiac function was evaluated in vivo and in vitro after 4 weeks. RESULTS: G-CSF mobilization increased the number of circulating EPCs. Compared to the control group, cardiac function in MI group was deteriorated, whereas that in the G-CSF treated group was significantly improved. Mobilization with G-CSF increased the serum level of VEGF and decreased CRP concentration. CONCLUSION: In rats with myocardial infarction, G-CSF promotes the mobilization of EPCs, augments the release of VEGF and improves cardiac function. EPC mobilization with G-CSF is a promising novel therapy for MI.

参考文献/References

［1］ Isner J, Asahara T. Angiogenesis and vasculogenesis as therapeutic strategies for postnatal neovascularization［J］. J Clin Invest, 1999, 103(9):1231-1236.

［2］ Kawamoto A, Gwon HC, Iwaguro H, et al. Therapeutic potential endothelial progenitor cells for of ex vivo expanded myocardial ischemia［J］. Circulation, 2001, 103(5):634-637.

［3］ Strauer BE, Brehm M, Zeus T, et al. Repair of infarcted myocardium by autologous intracoronary mononuclear bone marrow cell transplantation in humans［J］. Circulation, 2002, 106(15):1913-1918.

［4］ Perin EC, Dohmann HF, Borojevic R, et al. Transendocardial, autologous bone marrow cell transplantation for severe, chronic ischemic heart failure［J］. Circulation, 2003, 107(18):2294-2302.

［5］ Fuchs S, Satler LF, Kornowski R, et al. Catheter-based autologous bone marrow myocardial injection in no-option patients with advanced coronaryartery disease: a feasibility study［J］. J Am Coll Cardiol, 2003, 41(10):1721-1724.

［6］ Wang JS, Shum-Tim D, Galipeau J, et al. Marrow stromal cells for cellular cardiomyoplasty: feasibility and potential clinical advantages［J］. J Thorac Cardiovasc Surg, 2000, 120(5):999-1005.

［7］ Toma C, Pittenger MF, Cahill KS, et al. Human mesenchymal stem cells differentiate to a cardiomyocyte phenotype in the adult murine heart［J］. Circulation, 2002, 105(1):93-98.

［8］ Davani S, Marandin A, Mersin N, et al. Mesenchymal progenitor cells differentiate into an endothelial phenotype, enhance vascular density, and improve heart function in a rat cellular cardiomyoplasty model［J］. Circulation, 2003, 108(Suppl 1):II253-II258.

［9］ Orlic D, Kajstura J, Chimenti S, et al. Bone marrow cells regenerate infarcted myocardium［J］. Nature, 2001 ,410(6829):701-705.

［10］Verma S, Kuliszewski MA, Li SH, et al. C-reactive protein attenuates endothelial progenitor cell survival, differentiation and function［J］. Circulation, 2004, 109(7):2058-2067.

备注/Memo

备注/Memo:
收稿日期：2008-12-31.通讯作者：米卫东，教授，主要从事麻醉药理学研究Email:wwdd1962@yahoo.com.cn 作者简介：陈婷婷，主治医师，博士Email:chentt301@263.com
更新日期/Last Update: 2009-06-15