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|本期目录/Table of Contents|

心肌梗死后基质细胞衍生因子-1的表达及其意义(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第4期
页码:
471-474,479
栏目:
基础研究
出版日期:
2009-06-25

文章信息/Info

Title:
Expression of stromal cell-derived factor-1 after myocardial infarction and its significance
作者:
何庚戌1; 要彤2; 张浩3; 胡盛寿3; 张晓玲3; 魏英杰3
1.解放军第251医院胸心血管外科, 河北 张家口 075000；2.张家口北方学院第一附属医院心脏内科，河北 张家口 075000；3.中国协和医科大学阜外心血管病医院，卫生部心血管疾病再生医学重点实验室，北京 100037
Author(s):
HE Geng-xu1;  YAO Tong2;  ZHANG Hao3;  HU Sheng-shou3;  ZHANG Xiao-ling3;  WEI Ying-jie3
1.Department of Thoracic and Cardiovascular Surgery, PLA 251 Hospital, Zhangjiakou 075000, Hebei, China; 2.Department of Cardiology, First Affiliated Hospital, North College of Zhangjiakou, Zhangjiakou 075000, Hebei, China; 3.Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences & Beijing Union Medical College, PUMC, Beijing 100037, China
关键词:
基质细胞衍生因子-1; 心肌梗死; 细胞移植; 骨髓间充质干细胞
Keywords:
stromal cell-derived factor-1;  myocardial infarction;  cellular transplantation;  bone marrow mesenchymal stem cell
分类号:
R457.7
DOI:
-
文献标识码:
A
摘要:
目的 探讨大鼠心肌梗死(MI)后不同时间，梗死组织基质细胞衍生因子-1(SDF-1)的表达以及与骨髓间充质干细胞（MSC）迁移的关系，为细胞移植提供可靠依据。方法 在MI后不同时间，用PCR测定梗死组织中SDF-1表达的水平。提取梗死心肌组织提取液，在Costar Transwell 双层细胞培养皿观察MI后不同时间梗死心肌组织对MSC迁移能力的影响。结果 SDF-1在组织脏器内的表达存在差异，MI梗死心肌组织中SDF-1的表达呈现出时间变化的趋势，梗死后48 h 达到高峰水平。梗死后的心肌组织对MSC迁移的影响与SDF-1的表达呈相同的变化程序，梗死后48 h 对MSC的趋化作用最强。经SDF-1的受体CXCR4特异性阻滞剂处理后，MSC向梗死组织提取液迁移的能力受到明显抑制。结论 MI后的早期，局部组织中SDF-1的表达明显升高，并可在MI后2周内维持在一个相对高的水平，其对于移植细胞向梗死区域迁移具有重要的作用。
Abstract:
AIM: To explore the expression of stromal cell-derived factor-1 (SDF-1) in infarcted myocardial tissue in rats at different periods after myocardial infarction and its relationship to the migration of bone marrow mesenchymal stem cell (MSC) so as to provide reliable basis for cellular transplantation. METHODS: SDF-1 expression was measured by RT-PCR in different organs and the infarcted hearts at different postoperative times. The migratory ability of MSC to the supernatants of the infracted area was evaluated through double-chamber transwell culture plates. RESULTS: Myocardial SDF-1 expression was significantly increased and peaked at the second day and remained at the high level for about 2 weeks. MSCs migrating toward infarcted myocardial tissue supernatant showed the same time course as that of SDF-1 expression, and migrating MSCs were significantly inhibited by the inhibitor of CXCR4, the receptor of SDF-1. CONCLUSION: Myocardial SDF-1 expression increases in the early phase post-MI and remains at a high level for a short period, which may play an important role in chemoattracting the transplanted cells to the infarcted tissue.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2008-1-5.通讯作者：胡盛寿，主任医师，教授，主要从事终末期心脏病的治疗研究Email:huss@163bj.com 作者简介：何庚戌， 副主任医师，博士Email:hegengxu@yahoo.com.cn
更新日期/Last Update: 2009-06-15