我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

汉族人群花生四烯酸ω-羟化酶4F2基因编码区多态性位点的研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第4期
页码:
507-510
栏目:
临床研究
出版日期:
2009-06-25

文章信息/Info

Title:
Study on polymorphisms existing in coding region of ω-hydroxylase gene (CYP4F2) in a Han population
作者:
杨靖1; 任雨笙1; 汪道文2
1.第二军医大学附属长征医院心内科，上海 200003； 2.华中科技大学同济医学院附属同济医院心内科，湖北 武汉 430030
Author(s):
YANG Jing1;  Ren Yu-sheng1;  WANG Dao-wen2
1.Department of Cardiology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; 2.Department of Cardiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China
关键词:
花生四烯酸ω-羟化酶; CYP4F2; 多态性位点
Keywords:
arachidonic acid ω-hydroxylase;  CYP4F2;  SNP
分类号:
Q786
DOI:
-
文献标识码:
A
摘要:
目的 寻找汉族人群花生四烯酸细胞色素P450ω-羟化酶基因CYP4F2的编码区多态性位点分布情况。方法 采集50例血压正常、无血缘关系的汉族人外周静脉血白细胞；提取基因组DNA；设计特异性引物，PCR法分段扩增基因编码区序列，电泳检测后回收目的片断；测序，与参考序列比对后统计结果。 结果 CYP4F2共发现6个多态性位点，其中3个有意义：1个3′端非编码区的多态性位点G19026A，2个引起氨基酸改变的多态性位点T2013G和G18000A。其余为同义突变或者位于内含子区。结论 ①多态性位点存在种族差异；②突变位点大多数位于内含子区，或者为同义突变，仅有少数有意义；③发现的多态性位点可能引起蛋白质构象进而改变蛋白质功能，或者引起基因转录活性的改变。
Abstract:
AIM: To identify the characteristic single nucleotide polymorphisms (SNPs) existing in the coding region of arachidonic acid ω-hydroxylase gene, CYP4F2, in a Han population. METHODS: Genomic DNA was extracted from white blood cells of 50 unrelated Chinese Han normotensive subjects. Coding regions were amplified by PCR. After sequencing, the information was aligned and summarized. RESULTS: Six SNP sites in CYP4F2 were identified. There were three sense mutations including G19026A in the 3′-uncoding region, T2013G and G18000A, which caused corresponding amino acid changes (V-G, V-M), respectively. Others were in intron region or nonsynonymous. CONCLUSION: Multiple variants exist within or near the coding region of CYP4F2 gene in a Han population. SNPs are different in variant races and most SNPs are nonsense or in intron region. These SNPs or variants may change biological activity of ω-hydroxylases.

参考文献/References

［1］Jin R, Koop DR, Raucy JL, et al. Role of human CYP4F2 in hepatic catabolism of the proinflammatory agent leukotriene B4［J］. Arch Biochem Biophys, 1998, 359(1):89-98.

［2］ Kikuta Y, Miyauchi Y, Kusunose E, et al. Expression and molecular cloning of human liver leukotriene B4 omega-hydroxylase (CYP4F2) gene［J］. DNA Cell Biol, 1999, 18(9):723-730.

［3］ Kikuta Y, Kusunose E, Kusunose M. Characterization of human liver leukotriene B(4) omega-hydroxylase P450 (CYP4F2)［J］. J Biochem, 2000, 127(6):1047-1052.

［4］ Zhang X, Hardwick JP. Regulation of CYP4F2 leukotriene B4 omega-hydroxylase by retinoic acids in HepG2 cells［J］. Biochem Biophys Res Commun, 2000, 279(3):864-871.

［5］ Baker JR, Edwards RJ, Lasker JM, et al. Renal and hepatic accumulation of cadmium and lead in the expression of CYP4F2 and CYP2E1［J］. Toxicol Lett, 2005, 159(2):182-191.

［6］ Lasker JM, Chen WB, Wolf I, et al. Formation of 20-hydroxyeicosatetraenoic acid, a vasoactive and natriuretic eicosanoid, in human kidney. Role of Cyp4F2 and Cyp4A11［J］. J Biol Chem, 2000, 275(6):4118-4126.

［7］ Ma YH, Gebremedhin D, Schwartzman ML, et al. 20-Hydroxyeicosatetraenoic acid is an endogenous vasoconstrictor of canine renal arcuate arteries［J］. Circ Res, 1993, 72(1):126-136.

［8］ Harder DR, Campbell WB, Roman RJ, et al. Role of cytochrome P-450 enzymes and metabolites of arachidonic acid in the control of vascular tone［J］. J Vasc Res, 1995, 32(2):79-92.

［9］ Zou A P, Fleming JT, Falck JR, et al. 20-HETE is an endogenous inhibitor of the large-conductance Ca2+-activated K+ channel in renal arterioles［J］. Am J Physiol, 1996, 270(1 Pt 2):R228-R237.

［10］Jin HJ. Isolation of human CYP4F2 genomic DNA and its 5’ end regulatory region structure［J］. Arch Pharm Res, 1998, 21(1):35-40.

［11］Stec DE, Roman RJ, Flasch A, et al. Functional polymorphism in human CYP4F2 decreases 20-HETE production［J］. In Physiol Genomics, 2007, 30(1):74-81.

［12］Fava C, Montagnana M, Almgren P, et al. The V433M variant of the CYP4F2 is associated with ischemic stroke in male Swedes beyond its effect on blood pressure［J］. Hypertension, 2008, 52(2):373-380.

［13］Fu Z, Nakayama T, Sato N, et al. A haplotype of the CYP4F2 gene is associated with cerebral infarction in japanese men［J］. Am J Hypertens, 2008, 21(11):1216-1223.

备注/Memo

备注/Memo:
收稿日期：2008-10-21.作者简介：杨靖，住院医师，硕士Email:ema-co@163.com
更新日期/Last Update: 2009-06-15