我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

铁离子螯合剂去铁胺对巨噬细胞、泡沫细胞细胞外基质金属蛋白酶诱导因子表达的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第5期
页码:
601-605
栏目:
基础研究
出版日期:
2009-07-14

文章信息/Info

Title:
Iron chelator desferrioxamine enhances EMMPRIN expression in THP-1-derived macrophages and foam cells
作者:
范虞琪; 何奔; 王彬尧
上海交通大学医学院附属仁济医院心内科，上海 200001
Author(s):
FAN Yu-qi;  HE Ben;  WANG Bin-yao
Department of Cardiology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200001, China
关键词:
去铁胺; 细胞外基质金属蛋白酶诱导因子; 基质金属蛋白酶; 动脉粥样硬化
Keywords:
desferrioxamine;  extracellular matrix metalloproteinase inducer;  matrix metalloproteinase;  atherosclerosis
分类号:
Q559
DOI:
-
文献标识码:
A
摘要:
目的：观察铁负荷过低对巨噬细胞、泡沫细胞细胞外基质金属蛋白酶诱导因子(EMMPRIN)表达的影响。方法: 体外诱导THP-1单核细胞转化为巨噬细胞、泡沫细胞。实验细胞分为3组：对照组（正常巨噬细胞、泡沫细胞）、铁离子螯合剂去铁胺(DFO)刺激组、柠檬酸铁和DFO共刺激组。应用RT-PCR和Western blot测定巨噬细胞、泡沫细胞中EMMPRIN基因和蛋白的表达。用Western blot测定MMP-9蛋白的表达。用明胶酶谱法测定MMP-9的活性。结果: DFO刺激组中EMMPRIN基因及蛋白的水平、MMP-9蛋白表达的水平及活性均明显高于对照组（P<0.05，P<0.01）。柠檬酸铁逆转了DFO对EMMPRIN表达的上调作用。结论: 铁负荷过低可增加巨噬细胞及泡沫细胞中炎症因子的表达及活性，可能会促进心血管事件的发生。
Abstract:
AIM: To observe the effect of iron depletion on extracellular matrix metalloproteinase inducer (EMMPRIN) expression in macrophages and foam cells. METHODS: THP-1 monocytes were induced to macrophages and foam cells. Cells were then divided into three groups: 1)normal macrophages/foam cells as control, 2)cells treated with different concentrations of desferrioxamine(DFO), 3)cells co-treated with ferric citrates and DFO. EMMPRIN mRNA and protein expression were assayed by RT-PCR and Western blot. MMP-9 expression was assayed by Western blot and its invasive activity was assayed by gel zymography. RESULTS: DFO significantly enhanced EMMPRIN mRNA and protein expression in macrophages and foam cells and upregulated MMP-9 expression and invasive activity (P<0.05, P<0.01). CONCLUSION: Iron depletion therapy enhances production of inflammatory factors in macrophages and foam cells, which may cause deadly cardiovascular events.

参考文献/References

［1］ sullivan JL. Iron and sex difference in heart disease risk［J］. Lancet, 1981, 1(8233):1293-1294.

［2］Hansson GK. Inflammation，athemsclemsis，and coronary artery disease［J］. N Engl J Med, 2005, 352(16):1685-1695.

［3］ Inokubo Y, Hanada H, Ishizaka H, et al. Plasma levels of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 are increased in the coronary circulation in patients with acute coronary syndrome［J］. Am Heart J, 2001, 141(2):211-217.

［4］ Schmidt R, Bultmann A, Ungerer M, et al. Extracellular matrix metalloproteinase inducer regulates matrix metalloproteinase activity in cardiovascular cells: implications in acute myocardial infarction［J］. Circulation, 2006, 113(6):834-841.

［5］ 何清，王长谦，葛恒，等. 特异性抑制单核细胞中EMMPRIN基因表达的siRNA筛选和鉴定［J］. 上海交通大学学报（医学版）， 2007， 27(6):681-684.

［6］ Schmidt R, Bultmann A, Fischel S, et al. Extracellular matrix metalloproteinase inducer (CD147) is a novel receptor on platelets, activates platelets, and augments nuclear factor kappaB-dependent inflammation in monocytes［J］. Circ Res, 2008, 102(2):302-309.

［7］ Duffy SJ, Biegelsen ES, Holbrook M, et al. Iron chelation improves endothelial function in patients with coronary artery disease［J］. Circulation, 2001, 103(23):2799-2804.

［8］ Choi EY, Kim EC, Oh HM, et al. Iron chelator triggers inflammatory signals in human intestinal epithelial cells: involvement of p38 and extracellular signal-regulated kinase signaling pathways［J］. J Immunol, 2004, 172(11):7069-7077.

［9］ Lee SK, Lee J, Min SK, et al. Iron chelator differentially activates macrophage inflammatory protein-3alpha/CCL20 in immortalized and malignant human oral keratinocytes［J］. Arch Oral Biol, 2008, 53(9):801-809.

［10］Kaomongkolgit R, Cheepsunthorn P, Pavasant P, et al. Iron increases MMP-9 expression through activation of AP-1 via ERK/Akt pathway in human head and neck squamous carcinoma cells［J］. Oral Oncol, 2008, 44(6):587-594.

［11］周磊，曾锦章，沈玲红，等. 9一顺式维甲酸抑制PMA诱导人单核细胞系THP-1表达MMP-9［J］. 心脏杂志, 2007, 19(4):373-376.

备注/Memo

备注/Memo:
收稿日期：2009-2-25.基金项目：国家自然科学基金项目资助(30670880)；上海市科委基础研究项目资助(08XD1402600) 通讯作者：王彬尧,教授,主要从事动脉粥样硬化性心脏病的防治Email：binyaowang@hotmail.com 作者简介：范虞琪，住院医师，博士生Email：moricizine@gmail.com
更新日期/Last Update: 2009-07-22