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Title:: Protective effect of captopril and benazepril on myocardium in diabetic rats

作者:: 张晗; 熊世熙; 王海蓉; 宋陈芳; 曹建雷; 曹新营; 武汉大学中南医院心血管内科，湖北武汉 430071

Author(s):: ZHANG Han; XIONG Shi-xi; WANG Hai-rong; SONG Chen-fang; CAO Jian-lei; CAO Xin-ying; Department of Cardiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei, China

关键词:: 糖尿病心肌病; 心肌间质重构; 卡托普利; 贝那普利; Fas/FasL

Keywords:: diabetic myocardiopathy; myocardial interstitium remodeling; captopril; benazepril; Fas/FasL

分类号:: R972.4

DOI:: -

文献标识码:: A

摘要:: 目的: 探讨卡托普利与贝那普利对糖尿病大鼠心肌的保护作用。方法: 雄性SD大鼠60只随机分为空白对照组(n=15)和糖尿病组(n=45)。糖尿病组用链脲佐菌素(STZ，50 mg/kg)ip建模后，再随机分为糖尿病对照组、卡托普利50 mg/(kg·d)治疗组和贝那普利10 mg/(kg·d)治疗组。灌胃治疗9周后，处死全部大鼠，取心肌组织，用SP免疫组化染色法检测Ⅰ，Ⅲ型胶原的含量及Fas/FasL蛋白的表达率。电镜下观察心肌纤维及胶原的变化。结果: 与空白对照组相比，糖尿病对照组及两个治疗组的心脏指数明显增大(P<0.05)；Fas蛋白的表达率及Ⅰ，Ⅲ型胶原的表达增加(P<0.05)；FasL蛋白的表达率差异不显著。卡托普利和贝那普利治疗组，上述指标较糖尿病对照组有显著改善；但与贝那普利治疗组相比，卡托普利组心脏指数及Ⅰ，Ⅲ型胶原的含量改善效果较差(P<0.05)。结论: 糖尿病大鼠心肌中，有心肌细胞调亡和心肌间质重构发生。卡托普利与贝那普利可通过降低Fas和Ⅰ，Ⅲ型胶原的表达，明显改善心脏功能，但二者的效果稍有差别。

Abstract:: AIM: To explore the protective effects of captopril and benazepril on reversal of myocardial interstitium remodeling. METHODS: Sixty male Sprague Dawley rats were randomly divided into normal group (n=15) and streptozotocin (STZ)-induced diabetic group (n=45). Diabetic rats were randomly subdivided into diabetic control group, captopril-treated group and benazepril-treated group (15 rats in each group). Captopril or benazepril was administered each day at concentrations of 50 mg/kg and 10 mg/kg, respectively. After 9 weeks, all rats were sacrificed. SP immunohistochemical staining was used to detect the level of type I collagen, type III collagen and the expression rate of Fas/FasL protein. Transmission electron microscope was used to observe the changes of cardiocytes and collagen. RESULTS: Compared with the normal group, cardiac index, expression rate of Fas protein and level of type I and type III collagen in the diabetic group all significantly increased (P<0.05), whereas no difference was observed in the expression rate of FasL protein. Compared with the diabetic control group, indexes were all improved in the two treated groups, but the cardiac index and collagen in captopril-treated group was less effective than in the benazepril-treated group. CONCLUSION: Interstitial remodeling occurs in diabetic cardiomyopathy. Despite some slight differences, both captopril and benazepril improve heart function by reducing the expression of types I and III collagen and decreasing the expression rate of Fas protein.

参考文献/References

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卡托普利与贝那普利对糖尿病大鼠心肌的保护作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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