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|本期目录/Table of Contents|

自发性高血压大鼠心脏重构与ACE2 mRNA的表达

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第4期
页码:
517-519
栏目:
基础研究
出版日期:
2010-06-10

文章信息/Info

Title:
Relationship between cardiac remodeling and expression of angiotensin-converting enzyme 2 in spontaneously hypertensive rats
作者:
吴逸南1; 贺红2; 姜虹2; 葛志明2; 李方2; 张运2
1.山东中医药大学第二附属医院心内科，山东 济南 250001；2.山东大学齐鲁医院心内科，山东 济南 250012
Author(s):
WU Yi-nan1;  HE Hong2;  JIANG Hong2;  GE Zhi-ming2;  LI Fang2;  ZHANG Yun2
1.Department of Cardiology, Second Affiliated Hospital, Shandong Chinese Medical University, Jinan 250001, Shandong, China; 2.Department of Cardiology, Qilu Hospital, Shandong University, Jinan 250012, Shandong, China
关键词:
血管紧张素转换酶2; 自发性高血压; 心脏重构; 大鼠
Keywords:
angiotensin-converting enzyme 2;  spontaneously hypertensive rats;  cardiac remodeling
分类号:
R544.1
DOI:
-
文献标识码:
A
摘要:
目的: 观察不同月龄的自发性高血压大鼠(SHR)的心脏血管紧张素转换酶2(ACE2) mRNA表达水平，探讨心脏重构与ACE2的内在联系。方法: 将12周龄雄性SHR 18只和12周龄WKY Wistar-Kyoto rats大鼠18只随机分为两组，从WKY大鼠组和SHR组中各抽取9只处死，剩余的9只再喂养12周后处死。测量大鼠心脏的质量(HW)与体质量(BW)并计算HW/BW的比值。以实时定量RT-PCR法检测ACE2 mRNA的表达。结果: ①与同周龄WKY大鼠组比较，SHR组HW/BW的比值显著增加(P<0.01)；与12周龄SHR组比较，24周龄SHR组的HW/BW显著增加(P<0.05)。②与同周龄的WKY大鼠组比较，SHR组ACE2 mRNA的表达显著降低(P<0.01)；与12周龄的SHR组比较，24周龄的SHR组ACE2 mRNA的表达显著降低(P<0.01)。结论: 自发性高血压大鼠心脏重构伴随着心脏中ACE2 mRNA的表达下调。
Abstract:
AIM: To investigate the relationship of cardiac remodeling and expression of angiotensin-converting enzyme (ACE) 2 mRNA in spontaneously hypertensive rats (SHRs). METHODS: Eighteen male (12 weeks of age) SHRs and 18 male (12 weeks of age) Wistar Kyoto (WKY) rats were randomly divided into two groups: WKY control group (n=18) and SHR group (n=18). Nine rats were randomly selected from each group and sacrificed immediately, whereas the other nine rats in each group were sacrificed 12 weeks later. Heart weight/body weight (HW/BW) was measured and mRNA expression of ACE2 was determined by real-time quantitative RT-PCR in all rats. RESULTS: HW/BW in 12- and 24-week-old SHRs increased compared with WKY rats (P<0.01, P<0.01). HW/BW in 24-week-old SHR rats increased compared with 12-week-old SHRs (P<0.05). Myocardial expression of ACE2 mRNA in 12- and 24-week-old SHR rats was significantly lower than in WKY rats (P<0.01, P<0.01). Myocardial expression of ACE2 mRNA in 24-week-old SHRs was significantly lower than in 12-week-old SHRs (P<0.01). CONCLUSION: Cardiac remodeling is related to expressions of ACE2 mRNA in SHRs.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2009-02-24.作者简介：吴逸南，副主任医师，博士Email:wuyn_6@163.com
更新日期/Last Update: 2010-05-20