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Ⅱ型糖尿病大鼠心梗后非梗死区心室重构的研究

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第4期
页码:
520-523,636
栏目:
基础研究
出版日期:
2010-06-10

文章信息/Info

Title:
Ventricular remodeling in non-infarcted areas after myocardial infarction in type II diabetic rats
作者:
路军; 刘野; 郑强荪; 滕继伟; 李若愚; 张丽娜; 刘雄涛
第四军医大学唐都医院心内科,陕西 西安 710038
Author(s):
LU Jun;  LIU Ye;  ZHENG Qiang-sun;  TENG Ji-wei;  LI Ruo-yu;  ZHANG Li-na;  LIU Xiong-tao
Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi, China
关键词:
糖尿病; 心肌梗死; 心室重构; 大鼠
Keywords:
diabetes mellitus;  myocardial infarction;  ventricular remodeling;  rats
分类号:
R542.22
DOI:
-
文献标识码:
A
摘要:
目的: 对比观察糖尿病(DM)大鼠心肌梗死(MI)后非梗死区域心肌肥大、增殖分化和细胞凋亡的改变，探讨DM对MI后心室重构的影响。方法: 将实验大鼠分为正常对照组、糖尿病对照组、MI对照组及糖尿病MI组，从各组大鼠的左心室梗死周边取材，采用免疫组化染色法检测转化生长因子-β1(TGF-β1)、增殖细胞核抗原(PCNA)，用末端脱氧核苷酸转移酶介导的dUTP末端缺口标记法(TUNEL)检测凋亡细胞，并进行对比分析。结果: DM大鼠MI后非梗死区域存在着明显的心肌肥大、增殖分化和细胞凋亡。试验表明，糖尿病MI组大鼠梗死区周边凋亡细胞及PCNA、TGF-β1的阳性率均明显高于DM对照组及MI对照组(P<0.01)。DM对照组大鼠梗死区周边凋亡细胞的比例明显高于MI对照组(P<0.01)。结论: DM可增强TGF-β1和PCNA的表达及增加细胞凋亡，三者可能为DM大鼠MI后高心律失常及高死亡率的病理生理基础。
Abstract:
AIM: To explore the effect of diabetes on ventricular remodeling in non-infarcted areas after myocardial infarction in type II diabetic rats by comparing changes of cardiac hypertrophy (TGF-β1), proliferation differentiation (PCNA) and apoptosis (TUNEL). METHODS: Sprague Dawley (SD) rats were randomly divided into blank group, diabetic group, infarcted group and infarcted diabetic group. Samples were obtained from the infarcted diabetic rats in non-infarcted area. Using immunohistochemistry and computer image processing technique, TGF-β1, PCNA and TUNEL expressions were examined in different groups (blank, diabetic, infarcted, infarcted diabetic). RESULTS: After myocardial infarction, the positive rates of TUNEL, PCNA, TGF-β1 in non-infarcted areas in infarcted diabetic group were significantly higher than those in the blank group, diabetic group and infarcted group (P<0.01). Compared with the infarcted group, the positive rate of TUNEL in non-infarcted areas in diabetic group was significantly higher. CONCLUSION: Diabetes increased expressions of cardiac hypertrophy, proliferation and apoptosis in non-infarcted regions, which may be the pathophysiological basis of high morbidity of ventricular arrhythmia and high mortality after myocardial infarction in diabetes.

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备注/Memo

备注/Memo:
收稿日期：2009-07-27.通讯作者:郑强荪，主任医师，主要从事心律失常的临床和基础研究 Email:qingshu@fmmu.edu.cn 作者简介:路军，住院医师，硕士生Email:supermanlujun@vip.sina.com
更新日期/Last Update: 2010-05-20